LOCUS       pBSK?B-CAG-MCP-tdiRFP670-IRES-Neo.xdna    9280 bp      DNA     circular  
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     primer_bind     2172..2191
                     /label=T3
     primer_bind     complement(8665..8682)
                     /label=M13-fwd

     primer_bind     2134..2154
                     /label=M13-rev
     primer_bind     complement(8636..8655)
                     /label=T7
     primer_bind     3988..4007
                     /label=T7
     rep_origin      1138..1766
                     /label=ColE1 origin
     rep_origin      complement(8828..9268)
                     /label=F1 ori
     CDS             8753..8821
                     /label=LacZ alpha
     misc_binding    2106..2128
                     /label=LacO
     Promoter        59..87
                     /label=Amp prom
     Promoter        2072..2101
                     /label=lac
     intron          6526..6821
                     /label=synth int
     misc_feature    8436..8486
                     /label=SV40 early mRNA polyadenylation signal
     CDS             4058..4078
                     /label=NLS
     promoter        2854..3938
                     /label=CAG promoter
     CDS             4139..4492
                     /label=MCP
     CDS             4526..6460
                     /label=tdiRFP670
     CDS             4082..4108
                     /label=HA tag
     CDS             4109..4120
                     /label=FactorXa site
     misc_feature    4058..4081
                     /label=SV40 NLS1
     misc_feature    6870..7452
                     /label=internal ribosome entry site IRES
     misc_feature    7473..8276
                     /label=neoR KanR
ORIGIN
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 
      841 GTGGCAGCCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC 
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 
     1801 CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG 
     1861 AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG 
     1921 AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT 
     1981 GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG 
     2041 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT 
     2101 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG 
     2161 CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG 
     2221 GCCGCTCTAG AACTAGTGAG ACGTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 
     2281 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 
     2341 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 
     2401 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 
     2461 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 
     2521 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 
     2581 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC 
     2641 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA 
     2701 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG 
     2761 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT 
     2821 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC 
     2881 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC 
     2941 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC 
     3001 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG 
     3061 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG 
     3121 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG 
     3181 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC 
     3241 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG 
     3301 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC 
     3361 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC 
     3421 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG 
     3481 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT 
     3541 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG 
     3601 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC 
     3661 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT 
     3721 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG 
     3781 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG 
     3841 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA 
     3901 ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGA GCTCTCTGGC TAACTAGAGA 
     3961 ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTTG 
     4021 GTATCGACCT CGGATCGTCG CTAGCGATAT CATGGGCCCA AAAAAGAAAA GAAAAGTTGG 
     4081 CTACCCCTAC GACGTGCCCG ACTACGCCAT CGAAGGCCGC CATATGCTAG CCGTTAAAAT 
     4141 GGCTTCTAAC TTTACTCAGT TCGTTCTCGT CGACAATGGC GGAACTGGCG ACGTGACTGT 
     4201 CGCCCCAAGC AACTTCGCTA ACGGGATCGC TGAATGGATC AGCTCTAACT CGCGTTCACA 
     4261 GGCTTACAAA GTAACCTGTA GCGTTCGTCA GAGCTCTGCG CAGAATCGCA AATACACCAT 
     4321 CAAAGTCGAG GTGCCTAAAG GCGCCTGGCG TTCGTACTTA AATATGGAAC TAACCATTCC 
     4381 AATTTTCGCC ACGAATTCCG ACTGCGAGCT TATTGTTAAG GCAATGCAAG GTCTCCTAAA 
     4441 AGATGGAAAC CCGATTCCCT CAGCAATCGC AGCAAACTCC GGCATCTACG CCATGGCTTC 
     4501 TAACTTTACT CAGACCGGTG AATTCATGGC CAGAAAAGTG GACCTGACCA GTTGTGACCG 
     4561 AGAGCCGATA CACATTCCTG GCTCTATCCA GCCATGTGGG TGTTTGCTGG CCTGCGATGC 
     4621 CCAGGCAGTC AGGATTACTC GAATTACCGA AAATGCGGGT GCCTTCTTTG GCCGTGAAAC 
     4681 GCCCCGTGTC GGCGAACTCT TAGCCGATTA TTTCGGAGAA ACCGAGGCTC ACGCACTTAG 
     4741 GAACGCACTG GCGCAATCAA GCGATCCCAA ACGCCCTGCT CTCATCTTTG GGTGGAGAGA 
     4801 TGGACTGACA GGCCGTACTT TCGACATAAG CCTTCACCGG CATGACGGAA CCAGTATAAT 
     4861 TGAGTTTGAA CCGGCCGCCG CTGAGCAAGC AGACAACCCC CTTCGCCTGA CCAGACAAAT 
     4921 TATCGCCCGC ACTAAGGAGC TGAAATCGCT GGAAGAAATG GCCGCTCGTG TTCCCAGGTA 
     4981 TCTTCAGGCA ATGCTGGGTT ATCATAGGGT AATGCTCTAC AGATTCGCCG ACGATGGCTC 
     5041 TGGTATGGTA ATAGGGGAGG CCAAGCGATC CGACCTGGAG TCTTTTCTGG GGCAGCATTT 
     5101 CCCCGCATCT CTAGTACCCC AGCAGGCAAG GCTTTTGTAC CTCAAGAATG CCATCCGCGT 
     5161 GGTATCGGAC TCCCGCGGTA TTAGTAGCAG GATCGTTCCA GAACACGACG CCTCTGGAGC 
     5221 TGCGCTGGAT CTGTCTTTCG CTCACCTCCG ATCTATCAGC CCTTGTCATT TAGAGTTTCT 
     5281 GCGAAATATG GGGGTCAGCG CAAGTATGTC CCTGAGCATA ATAATCGATG GAACACTATG 
     5341 GGGCCTAATA ATTTGCCATC ATTACGAACC TAGAGCAGTA CCAATGGCAC AGCGAGTGGC 
     5401 CGCCGAAATG TTCGCCGATT TTTTGTCTCT TCATTTTACT GCCGCTCACC ATCAGCGCGG 
     5461 CCACGGGACT GGCTCGACAG GTTCTGGCAG TAGTGGGACT GCTTCATCAG AAGATAATAA 
     5521 CATGGCCATG GCCAGAAAAG TGGACCTGAC CAGTTGTGAC CGAGAGCCGA TACACATTCC 
     5581 TGGCTCTATC CAGCCATGTG GGTGTTTGCT GGCCTGCGAT GCCCAGGCAG TCAGGATTAC 
     5641 TCGAATTACC GAAAATGCGG GTGCCTTCTT TGGCCGTGAA ACGCCCCGTG TCGGCGAACT 
     5701 CTTAGCCGAT TATTTCGGAG AAACCGAGGC TCACGCACTT AGGAACGCAC TGGCGCAATC 
     5761 AAGCGATCCC AAACGCCCTG CTCTCATCTT TGGGTGGAGA GATGGACTGA CAGGCCGTAC 
     5821 TTTCGACATA AGCCTTCACC GGCATGACGG AACCAGTATA ATTGAGTTTG AACCGGCCGC 
     5881 CGCTGAGCAA GCAGACAACC CCCTTCGCCT GACCAGACAA ATTATCGCCC GCACTAAGGA 
     5941 GCTGAAATCG CTGGAAGAAA TGGCCGCTCG TGTTCCCAGG TATCTTCAGG CAATGCTGGG 
     6001 TTATCATAGG GTAATGCTCT ACAGATTCGC CGACGATGGC TCTGGTATGG TAATAGGGGA 
     6061 GGCCAAGCGA TCCGACCTGG AGTCTTTTCT GGGGCAGCAT TTCCCCGCAT CTCTAGTACC 
     6121 CCAGCAGGCA AGGCTTTTGT ACCTCAAGAA TGCCATCCGC GTGGTATCGG ACTCCCGCGG 
     6181 TATTAGTAGC AGGATCGTTC CAGAACACGA CGCCTCTGGA GCTGCGCTGG ATCTGTCTTT 
     6241 CGCTCACCTC CGATCTATCA GCCCTTGTCA TTTAGAGTTT CTGCGAAATA TGGGGGTCAG 
     6301 CGCAAGTATG TCCCTGAGCA TAATAATCGA TGGAACACTA TGGGGCCTAA TAATTTGCCA 
     6361 TCATTACGAA CCTAGAGCAG TACCAATGGC ACAGCGAGTG GCCGCCGAAA TGTTCGCCGA 
     6421 TTTTTTGTCT CTTCATTTTA CTGCCGCTCA CCATCAGCGC GGATCCGCGT TTAAACTCGA 
     6481 GGTTAATTAA TGAGCGGCCG CATAGATAAC TGATCCAGTG TGCTGGAATT AATTCGCTGT 
     6541 CTGCGAGGGC CAGCTGTTGG GGTGAGTACT CCCTCTCAAA AGCGGGCATG ACTTCTGCGC 
     6601 TAAGATTGTC AGTTTCCAAA AACGAGGAGG ATTTGATATT CACCTGGCCC GCGGTGATGC 
     6661 CTTTGAGGGT GGCCGCGTCC ATCTGGTCAG AAAAGACAAT CTTTTTGTTG TCAAGCTTGA 
     6721 GGTGTGGCAG GCTTGAGATC TGGCCATACA CTTGAGTGAC AATGACATCC ACTTTGCCTT 
     6781 TCTCTCCACA GGTGTCCACT CCCAGGTCCA ACTGCAGGTC GAGCATGCAT CTAGGGCGGC 
     6841 CAATTCCGCC CCTCTCCCCC CCCCCCTTTT CCCTCCCCCC CCCCTAACGT TACTGGCCGA 
     6901 AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG 
     6961 TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG 
     7021 GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT 
     7081 CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC 
     7141 CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA 
     7201 AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG 
     7261 CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG 
     7321 GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA 
     7381 CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAAGCT 
     7441 TGCCACAACC CGGGATAATT CCTGCAGCCA ATATGGGATC GGCCATTGAA CAAGATGGAT 
     7501 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 
     7561 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 
     7621 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 
     7681 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 
     7741 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 
     7801 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 
     7861 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 
     7921 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 
     7981 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGATGAT CTCGTCGTGA 
     8041 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA 
     8101 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 
     8161 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 
     8221 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGGGG 
     8281 ATCAATTCTC TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG 
     8341 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG 
     8401 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     8461 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     8521 TATCTTAACG CGTCGAGTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 
     8581 TCATGTCTGT ATACCCGTCT CGTCGACCTC GAGGGGGGGC CCGGTACCCA ATTCGCCCTA 
     8641 TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 
     8701 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA 
     8761 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG 
     8821 GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 
     8881 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 
     8941 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 
     9001 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 
     9061 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 
     9121 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 
     9181 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 
     9241 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG 
//