LOCUS       pBSK?B-TRE-dCas9-EGFP.xdna    8543 bp      DNA     circular 
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     primer_bind     2172..2191
                     /label=T3
     primer_bind     complement(7928..7945)
                     /label=M13-fwd
     primer_bind     2134..2154
                     /label=M13-rev
     primer_bind     complement(7899..7918)
                     /label=T7
     rep_origin      1138..1766
                     /label=ColE1 origin
     rep_origin      complement(8091..8531)
                     /label=F1 ori
     CDS             8016..8084
                     /label=LacZ alpha
     misc_binding    2106..2128
                     /label=LacO
     CDS             6878..7591
                     /label=EGFP
     Promoter        59..87
                     /label=Amp prome
     Promoter        2072..2101
                     /label=lac
     CDS             2633..2653
                     /label=NLS
     CDS             6767..6787
                     /label=NLS
     CDS             2663..6736
                     /label=dCas9
     promoter        2248..2499
                     /label=Tet response element
     polyA_signal    7647..7850
                     /label=pGH_PA_terminator
     CDS             2603..2629
                     /label=HA tag
ORIGIN
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 
      841 GTGGCAGCCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC 
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 
     1801 CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG 
     1861 AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG 
     1921 AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT 
     1981 GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG 
     2041 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT 
     2101 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG 
     2161 CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG 
     2221 GCCGCTCTAG AACTAGTCGT CTTCACTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA 
     2281 TGTCGAGTTT ACTCCCTATC AGTGATAGAG AACGATGTCG AGTTTACTCC CTATCAGTGA 
     2341 TAGAGAACGT ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTACT 
     2401 CCCTATCAGT GATAGAGAAC GTATGTCGAG TTTATCCCTA TCAGTGATAG AGAACGTATG 
     2461 TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GGTAGGCGTG TACGGTGGGA 
     2521 GGCCTATATA AGCAGAGCTC ATTTAGTGAA CCGTCAGATC GCCTGGAGAA TTCGAGCTCG 
     2581 GTACCCGACC GGTGCCACCA TGTACCCATA CGATGTTCCA GATTACGCTT CGCCGAAGAA 
     2641 AAAGCGCAAG GTCGAAGCGT CCGACAAGAA GTACAGCATC GGCCTGGCCA TCGGCACCAA 
     2701 CTCTGTGGGC TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT 
     2761 GCTGGGCAAC ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGAGCCC TGCTGTTCGA 
     2821 CAGCGGCGAA ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG 
     2881 ACGGAAGAAC CGGATCTGCT ATCTGCAAGA GATCTTCAGC AACGAGATGG CCAAGGTGGA 
     2941 CGACAGCTTC TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA 
     3001 GCGGCACCCC ATCTTCGGCA ACATCGTGGA CGAGGTGGCC TACCACGAGA AGTACCCCAC 
     3061 CATCTACCAC CTGAGAAAGA AACTGGTGGA CAGCACCGAC AAGGCCGACC TGCGGCTGAT 
     3121 CTATCTGGCC CTGGCCCACA TGATCAAGTT CCGGGGCCAC TTCCTGATCG AGGGCGACCT 
     3181 GAACCCCGAC AACAGCGACG TGGACAAGCT GTTCATCCAG CTGGTGCAGA CCTACAACCA 
     3241 GCTGTTCGAG GAAAACCCCA TCAACGCCAG CGGCGTGGAC GCCAAGGCCA TCCTGTCTGC 
     3301 CAGACTGAGC AAGAGCAGAC GGCTGGAAAA TCTGATCGCC CAGCTGCCCG GCGAGAAGAA 
     3361 GAATGGCCTG TTCGGCAACC TGATTGCCCT GAGCCTGGGC CTGACCCCCA ACTTCAAGAG 
     3421 CAACTTCGAC CTGGCCGAGG ATGCCAAACT GCAGCTGAGC AAGGACACCT ACGACGACGA 
     3481 CCTGGACAAC CTGCTGGCCC AGATCGGCGA CCAGTACGCC GACCTGTTTC TGGCCGCCAA 
     3541 GAACCTGTCC GACGCCATCC TGCTGAGCGA CATCCTGAGA GTGAACACCG AGATCACCAA 
     3601 GGCCCCCCTG AGCGCCTCTA TGATCAAGAG ATACGACGAG CACCACCAGG ACCTGACCCT 
     3661 GCTGAAAGCT CTCGTGCGGC AGCAGCTGCC TGAGAAGTAC AAAGAGATTT TCTTCGACCA 
     3721 GAGCAAGAAC GGCTACGCCG GCTACATTGA CGGCGGAGCC AGCCAGGAAG AGTTCTACAA 
     3781 GTTCATCAAG CCCATCCTGG AAAAGATGGA CGGCACCGAG GAACTGCTCG TGAAGCTGAA 
     3841 CAGAGAGGAC CTGCTGCGGA AGCAGCGGAC CTTCGACAAC GGCAGCATCC CCCACCAGAT 
     3901 CCACCTGGGA GAGCTGCACG CCATTCTGCG GCGGCAGGAA GATTTTTACC CATTCCTGAA 
     3961 GGACAACCGG GAAAAGATCG AGAAGATCCT GACCTTCCGC ATCCCCTACT ACGTGGGCCC 
     4021 TCTGGCCAGG GGAAACAGCA GATTCGCCTG GATGACCAGA AAGAGCGAGG AAACCATCAC 
     4081 CCCCTGGAAC TTCGAGGAAG TGGTGGACAA GGGCGCTTCA GCCCAGAGCT TCATCGAGCG 
     4141 GATGACCAAC TTCGATAAGA ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC ACAGCCTGCT 
     4201 GTACGAGTAC TTCACCGTGT ATAACGAGCT GACCAAAGTG AAATACGTGA CCGAGGGAAT 
     4261 GAGAAAGCCC GCCTTCCTGA GCGGCGAGCA GAAAAAGGCC ATCGTGGACC TGCTGTTCAA 
     4321 GACCAACCGG AAAGTGACCG TGAAGCAGCT GAAAGAGGAC TACTTCAAGA AAATCGAGTG 
     4381 CTTCGACTCC GTGGAAATCT CCGGCGTGGA AGATCGGTTC AACGCCTCCC TGGGCACATA 
     4441 CCACGATCTG CTGAAAATTA TCAAGGACAA GGACTTCCTG GACAATGAGG AAAACGAGGA 
     4501 CATTCTGGAA GATATCGTGC TGACCCTGAC ACTGTTTGAG GACAGAGAGA TGATCGAGGA 
     4561 ACGGCTGAAA ACCTATGCCC ACCTGTTCGA CGACAAAGTG ATGAAGCAGC TGAAGCGGCG 
     4621 GAGATACACC GGCTGGGGCA GGCTGAGCCG GAAGCTGATC AACGGCATCC GGGACAAGCA 
     4681 GTCCGGCAAG ACAATCCTGG ATTTCCTGAA GTCCGACGGC TTCGCCAACA GAAACTTCAT 
     4741 GCAGCTGATC CACGACGACA GCCTGACCTT TAAAGAGGAC ATCCAGAAAG CCCAGGTGTC 
     4801 CGGCCAGGGC GATAGCCTGC ACGAGCACAT TGCCAATCTG GCCGGCAGCC CCGCCATTAA 
     4861 GAAGGGCATC CTGCAGACAG TGAAGGTGGT GGACGAGCTC GTGAAAGTGA TGGGCCGGCA 
     4921 CAAGCCCGAG AACATCGTGA TCGAAATGGC CAGAGAGAAC CAGACCACCC AGAAGGGACA 
     4981 GAAGAACAGC CGCGAGAGAA TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC TGGGCAGCCA 
     5041 GATCCTGAAA GAACACCCCG TGGAAAACAC CCAGCTGCAG AACGAGAAGC TGTACCTGTA 
     5101 CTACCTGCAG AATGGGCGGG ATATGTACGT GGACCAGGAA CTGGACATCA ACCGGCTGTC 
     5161 CGACTACGAT GTGGACGCCA TCGTGCCTCA GAGCTTTCTG AAGGACGACT CCATCGACAA 
     5221 CAAGGTGCTG ACCAGAAGCG ACAAGAACCG GGGCAAGAGC GACAACGTGC CCTCCGAAGA 
     5281 GGTCGTGAAG AAGATGAAGA ACTACTGGCG GCAGCTGCTG AACGCCAAGC TGATTACCCA 
     5341 GAGAAAGTTC GACAATCTGA CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC TGGATAAGGC 
     5401 CGGCTTCATC AAGAGACAGC TGGTGGAAAC CCGGCAGATC ACAAAGCACG TGGCACAGAT 
     5461 CCTGGACTCC CGGATGAACA CTAAGTACGA CGAGAATGAC AAGCTGATCC GGGAAGTGAA 
     5521 AGTGATCACC CTGAAGTCCA AGCTGGTGTC CGATTTCCGG AAGGATTTCC AGTTTTACAA 
     5581 AGTGCGCGAG ATCAACAACT ACCACCACGC CCACGACGCC TACCTGAACG CCGTCGTGGG 
     5641 AACCGCCCTG ATCAAAAAGT ACCCTAAGCT GGAAAGCGAG TTCGTGTACG GCGACTACAA 
     5701 GGTGTACGAC GTGCGGAAGA TGATCGCCAA GAGCGAGCAG GAAATCGGCA AGGCTACCGC 
     5761 CAAGTACTTC TTCTACAGCA ACATCATGAA CTTTTTCAAG ACCGAGATTA CCCTGGCCAA 
     5821 CGGCGAGATC CGGAAGCGGC CTCTGATCGA GACAAACGGC GAAACCGGGG AGATCGTGTG 
     5881 GGATAAGGGC CGGGATTTTG CCACCGTGCG GAAAGTGCTG AGCATGCCCC AAGTGAATAT 
     5941 CGTGAAAAAG ACCGAGGTGC AGACAGGCGG CTTCAGCAAA GAGTCTATCC TGCCCAAGAG 
     6001 GAACAGCGAT AAGCTGATCG CCAGAAAGAA GGACTGGGAC CCTAAGAAGT ACGGCGGCTT 
     6061 CGACAGCCCC ACCGTGGCCT ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA AGGGCAAGTC 
     6121 CAAGAAACTG AAGAGTGTGA AAGAGCTGCT GGGGATCACC ATCATGGAAA GAAGCAGCTT 
     6181 CGAGAAGAAT CCCATCGACT TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA AAAAGGACCT 
     6241 GATCATCAAG CTGCCTAAGT ACTCCCTGTT CGAGCTGGAA AACGGCCGGA AGAGAATGCT 
     6301 GGCCTCTGCC GGCGAACTGC AGAAGGGAAA CGAACTGGCC CTGCCCTCCA AATATGTGAA 
     6361 CTTCCTGTAC CTGGCCAGCC ACTATGAGAA GCTGAAGGGC TCCCCCGAGG ATAATGAGCA 
     6421 GAAACAGCTG TTTGTGGAAC AGCACAAGCA CTACCTGGAC GAGATCATCG AGCAGATCAG 
     6481 CGAGTTCTCC AAGAGAGTGA TCCTGGCCGA CGCTAATCTG GACAAAGTGC TGTCCGCCTA 
     6541 CAACAAGCAC CGGGATAAGC CCATCAGAGA GCAGGCCGAG AATATCATCC ACCTGTTTAC 
     6601 CCTGACCAAT CTGGGAGCCC CTGCCGCCTT CAAGTACTTT GACACCACCA TCGACCGGAA 
     6661 GAGGTACACC AGCACCAAAG AGGTGCTGGA CGCCACCCTG ATCCACCAGA GCATCACCGG 
     6721 CCTGTACGAG ACACGGATCG ACCTGTCTCA GCTGGGAGGC GACAGCCCCA AGAAGAAGAG 
     6781 AAAGGTGGAG GCCAGCCTCT CGAGGCCGGA CCCCGCGCTG GCCGCACTCG GAGGCGGCGG 
     6841 AAGCGGTGGC GGAGGGTCAG GCGGAGGTGG ATCCATGGTG AGCAAGGGCG AGGAGCTGTT 
     6901 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG 
     6961 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG 
     7021 CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT 
     7081 GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT 
     7141 GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC 
     7201 CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT 
     7261 CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA 
     7321 CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG 
     7381 CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT 
     7441 CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG 
     7501 CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG 
     7561 GATCACTCTC GGCATGGACG AGCTGTACAA GTGATCTAGA ATGCTGATGG GCTAGCTAAG 
     7621 AATTCCTAGA GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 
     7681 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 
     7741 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 
     7801 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGAGAAT AGCAGGCATG CTGGGGAGAA 
     7861 GACGATCGAC CTCGAGGGGG GGCCCGGTAC CCAATTCGCC CTATAGTGAG TCGTATTACG 
     7921 CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 
     7981 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 
     8041 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGACGCG CCCTGTAGCG 
     8101 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8161 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8221 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8281 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     8341 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     8401 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     8461 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA 
     8521 AAATATTAAC GCTTACAATT TAG
//