LOCUS       pKLV-U6gRNA-EF(BbsI)-PGKpuro.xdna    8122 bp      DNA     circular   
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             3953..5323
                     /label=Puro2ABFP
     primer_bind     complement(5932..5951)
                     /label=T3
     primer_bind     599..616
                     /label=M13-fwd
     primer_bind     complement(5969..5989)
                     /label=M13-rev
     primer_bind     626..645
                     /label=T7
     rep_origin      complement(6357..6985)
                     /label=ColE1 origin
     rep_origin      13..453
                     /label=F1 ori
     CDS             complement(460..528)
                     /label=LacZ alpha
     misc_binding    complement(5995..6017)
                     /label=LacO
     CDS             complement(7137..7796)
                     /label=AmpR
     Promoter        3412..3751
                     /label=mPGK Prom
     Promoter        complement(8036..8064)
                     /label=Amp prom
     Promoter        complement(6022..6051)
                     /label=lac
     LTR             1236..1416
                     /label=HIV-1 5 LTR
     misc_feature    1527..1571
                     /label=HIV-1 psi pack
     misc_feature    2087..2320
                     /label=RRE
     polyA_signal    5677..5795
                     /label=BGH pA truncated
     enhancer        719..1006
                     /label=CAG_enhancer
     promoter        664..1216
                     /label=CMV_immearly_promoter
     misc_feature    1236..1416
                     /label=HIV-1_5_LTR
     CDS             3953..4540
                     /label=Puro variant
     misc_feature    2851..2866
                     /label=cPPT
     misc_feature    5388..5403
                     /label=cPPT
     misc_feature    5388..5409
                     /label=U3PPT
     misc_feature    5458..5658
                     /label=truncHIV-1_3 LTR
     misc_feature    5458..5658
                     /label=HIV-1_5_LTR Feature
     promoter        3024..3256
                     /label=hU6 promoter
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG 
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG 
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 
      601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGACTAGTTA 
      661 TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC 
      721 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC 
      781 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT 
      841 GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC 
      901 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC 
      961 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT 
     1021 GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC 
     1081 AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT 
     1141 TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG 
     1201 GGAGGTCTAT ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT 
     1261 GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC 
     1321 CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC 
     1381 TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA 
     1441 AGCGAAAGGG AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC 
     1501 GGCAAGAGGC GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA 
     1561 GAAGGAGAGA GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG 
     1621 GGAAAAAATT CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG 
     1681 GGCAAGCAGG GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG 
     1741 CTGTAGACAA ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG 
     1801 ATCATTATAT AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA 
     1861 CACCAAGGAA GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA 
     1921 GCAAGCGGCC GGCCGCGCTG ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA 
     1981 GAAGTGAATT ATATAAATAT AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA 
     2041 AGGCAAAGAG AAGAGTGGTG CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC 
     2101 TTGGGTTCTT GGGAGCAGCA GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC 
     2161 AGGCCAGACA ATTATTGTCT GGTATAGTGC AGCAGCAGAA CAATTTGCTG AGGGCTATTG 
     2221 AGGCGCAACA GCATCTGTTG CAACTCACAG TCTGGGGCAT CAAGCAGCTC CAGGCAAGAA 
     2281 TCCTGGCTGT GGAAAGATAC CTAAAGGATC AACAGCTCCT GGGGATTTGG GGTTGCTCTG 
     2341 GAAAACTCAT TTGCACCACT GCTGTGCCTT GGAATGCTAG TTGGAGTAAT AAATCTCTGG 
     2401 AACAGATTTG GAATCACACG ACCTGGATGG AGTGGGACAG AGAAATTAAC AATTACACAA 
     2461 GCTTAATACA CTCCTTAATT GAAGAATCGC AAAACCAGCA AGAAAAGAAT GAACAAGAAT 
     2521 TATTGGAATT AGATAAATGG GCAAGTTTGT GGAATTGGTT TAACATAACA AATTGGCTGT 
     2581 GGTATATAAA ATTATTCATA ATGATAGTAG GAGGCTTGGT AGGTTTAAGA ATAGTTTTTG 
     2641 CTGTACTTTC TATAGTGAAT AGAGTTAGGC AGGGATATTC ACCATTATCG TTTCAGACCC 
     2701 ACCTCCCAAC CCCGAGGGGA CCCGACAGGC CCGAAGGAAT AGAAGAAGAA GGTGGAGAGA 
     2761 GAGACAGAGA CAGATCCATT CGATTAGTGA ACGGATCGGC ACTGCGTGCG CCAATTCTGC 
     2821 AGACAAATGG CAGTATTCAT CCACAATTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT 
     2881 GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA 
     2941 CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTGG 
     3001 TTAGTACCGG GCCCGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT ATACGATACA 
     3061 AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA TTAGTACAAA 
     3121 ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA TTATGTTTTA 
     3181 AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 
     3241 TCTTGTGGAA AGGACGAAAC ACCGGGTCTT CGAGAAGACC TGTTTAAGAG CTATGCTGGA 
     3301 AACAGCATAG CAAGTTTAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG 
     3361 TCGGTGCTTT TTTGTTTTAG AGCTAGAAAT AGCAAGTTGG ATCCAATTCT ACCGGGTAGG 
     3421 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG 
     3481 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC 
     3541 GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT 
     3601 CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC 
     3661 TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG 
     3721 CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC 
     3781 CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG 
     3841 CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC 
     3901 TCCGGGCCTT TCGACCTGCA TCCATCTAGA TCTCGAGCAG CTGAAGCTTA CCATGACCGA 
     3961 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT 
     4021 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT 
     4081 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA 
     4141 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA 
     4201 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT 
     4261 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG 
     4321 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT 
     4381 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC 
     4441 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA 
     4501 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCG GCGGCGGGTC 
     4561 CGGAGGAGAG GGCAGAGGAA GTCTCCTAAC ATGCGGTGAC GTGGAGGAGA ATCCTGGCCC 
     4621 AATGAGCGAG CTGATTAAGG AGAACATGCA CATGAAGCTG TACATGGAGG GCACCGTGGA 
     4681 CAACCATCAC TTCAAGTGCA CATCCGAGGG CGAAGGCAAG CCCTACGAGG GCACCCAGAC 
     4741 CATGAGAATC AAGGTGGTCG AGGGCGGCCC TCTCCCCTTC GCCTTCGACA TCCTGGCTAC 
     4801 TAGCTTCCTC TACGGCAGCA AGACCTTCAT CAACCACACC CAGGGCATCC CCGACTTCTT 
     4861 CAAGCAGTCC TTCCCTGAGG GCTTCACATG GGAGAGAGTC ACCACATACG AGGACGGGGG 
     4921 CGTGCTGACC GCTACCCAGG ACACCAGCCT CCAGGACGGC TGCCTCATCT ACAACGTCAA 
     4981 GATCAGAGGG GTGAACTTCA CATCCAACGG CCCTGTGATG CAGAAGAAAA CACTCGGCTG 
     5041 GGAGGCCTTC ACCGAGACGC TGTACCCCGC TGACGGCGGC CTGGAAGGCA GAAACGACAT 
     5101 GGCCCTGAAG CTCGTGGGCG GGAGCCATCT GATCGCAAAC ATCAAGACCA CATATAGATC 
     5161 CAAGAAACCC GCTAAGAACC TCAAGATGCC TGGCGTCTAC TATGTGGACT ACAGACTGGA 
     5221 AAGAATCAAG GAGGCCAACA ACGAGACCTA CGTCGAGCAG CACGAGGTGG CAGTGGCCAG 
     5281 ATACTGCGAC CTCCCTAGCA AACTGGGGCA CAAGCTTAAT TGAGCGGCCG CTAGGTACCT 
     5341 TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG 
     5401 GGACTGGAAG GGCTAATTCA CTCCCAAAGA AGTCAAGATC TGCTTTTTGC CTGTACTGGG 
     5461 TCTCTCTGGT TAGACCAGAG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG 
     5521 GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG 
     5581 TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG 
     5641 TGTGGAAAAT CTCTAGCAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC 
     5701 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 
     5761 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 
     5821 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGT CAATAGCAGG 
     5881 CATGCTGGGG ATGCGGTGGG CTCTATGGGC GGCCGTTAAT TAAGCTTTTG TTCCCTTTAG 
     5941 TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 
     6001 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 
     6061 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 
     6121 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 
     6181 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 
     6241 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 
     6301 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 
     6361 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 
     6421 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 
     6481 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 
     6541 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 
     6601 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 
     6661 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 
     6721 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 
     6781 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG 
     6841 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 
     6901 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 
     6961 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 
     7021 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 
     7081 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 
     7141 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 
     7201 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 
     7261 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 
     7321 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 
     7381 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 
     7441 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 
     7501 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 
     7561 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 
     7621 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 
     7681 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 
     7741 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 
     7801 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 
     7861 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 
     7921 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 
     7981 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8041 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8101 ACATTTCCCC GAAAAGTGCC AC
//