LOCUS       Addgene62418            2025 bp    DNA     circular UNA 13-APR-2015
DEFINITION  MXS_minCMV.
ACCESSION   urn.local...1420721389987.30
VERSION     urn.local...1420721389987.30
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1643..1662
                     /note="Geneious type: DNA probe bind"
                     /label="DNA probe binding site"
     gene            complement(1048..1908)
                     /gene="bla"
                     /label="bla gene"
     misc_feature    18^19
                     /note="Geneious type: origin"
                     /label="origin"
     promoter        19..87
                     /label="minCMV"
     rep_origin      complement(289..877)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label="rep origin"
     sig_peptide     complement(1840..1908)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
                     /label="bla signal peptide"
     misc_RNA        complement(289..841)
                     /note="RNAII transcript (complementary strand)"
                     /label="RNA"
     misc_RNA        731..838
                     /note="RNAI transcript"
                     /label="RNAI transcript"
     -10_signal      717..722
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label="RNAI promoter (clockwise); GCTACA -10 signal"
     -10_signal      complement(1952..1957)
                     /note="bla promoter (counter-clockwise); GAGACA"
                     /label="-10 signal"
     -10_signal      complement(851..856)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label="-10 signal"
     -35_signal      695..700
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label="RNAI promoter (clockwise); TTGAAG -35 signal"
     -35_signal      complement(872..877)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label="-35 signal"
     -35_signal      complement(1973..1978)
                     /note="bla promoter (counter-clockwise); TTCAAA"
                     /label="-35 signal"
ORIGIN      
        1 aattacgcgt aactcgagta tgtcgaggtg gcgtgtacgg tgggaggcct atataagcag
       61 agctcgttta gtgaaccgtc agatcgcctg gagaattcag atctcagctg gtcgaccatg
      121 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      181 ggcggtaata cggttatata cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      241 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      301 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      361 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      421 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      481 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      541 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      601 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      661 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      721 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      781 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      841 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      901 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      961 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1021 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1081 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1141 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1201 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1261 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1321 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1381 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1441 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1501 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1561 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1621 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1681 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1741 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1801 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     1861 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     1921 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     1981 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccg
//
