LOCUS Addgene62438 3498 bp DNA circular UNA 13-APR-2015 DEFINITION MXS_PGK::NeoR-bGHpA. ACCESSION urn.local...1420721390013.36 VERSION urn.local...1420721390013.36 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 540..547 /label="Kozak" polyA_signal <1359..>1583 /label="bGHpA" promoter 26..533 /label="PGK" rep_origin 2742..3330 /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" /label="rep origin" sig_peptide 1711..1779 /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." /label="bla signal peptide" gene 1711..2571 /gene="bla" /label="bla gene" gene 548..1347 /label="NeoR" misc_RNA 2778..3330 /note="RNAII transcript (complementary strand)" /label="RNA" misc_RNA complement(2781..2888) /note="RNAI transcript" /label="RNAI transcript" -10_signal complement(2897..2902) /note="RNAI promoter (clockwise); GCTACA" /label="RNAI promoter (clockwise); GCTACA -10 signal" -10_signal 1662..1667 /note="bla promoter (counter-clockwise); GAGACA" /label="-10 signal" -10_signal 2763..2768 /note="RNAII promoter (counter-clockwise); CGTAAT" /label="-10 signal" -35_signal complement(2919..2924) /note="RNAI promoter (clockwise); TTGAAG" /label="RNAI promoter (clockwise); TTGAAG -35 signal" -35_signal 2742..2747 /note="RNAII promoter (counter-clockwise); TTGAGA" /label="-35 signal" -35_signal 1641..1646 /note="bla promoter (counter-clockwise); TTCAAA" /label="-35 signal" ORIGIN 1 catgatatca aacgcgtaac tcgagtctac cgggtagggg aggcgctttt cccaaggcag 61 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg 121 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc 181 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg 241 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc 301 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc 361 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg 421 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa 481 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacgtcgagt 541 cgccaccatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc cggccgcttg 601 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 661 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 721 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt 781 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 841 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 901 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 961 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 1021 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 1081 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 1141 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 1201 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 1261 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 1321 cttctatcgc cttcttgacg agttcttctg aggtcgagct gtgccttcta gttgccagcc 1381 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 1441 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 1501 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 1561 tggggatgcg gtgggctcta tggtcgacca attcggggaa atgtgcgcgg aacccctatt 1621 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 1681 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 1741 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 1801 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 1861 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 1921 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 1981 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 2041 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 2101 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 2161 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 2221 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 2281 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 2341 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 2401 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 2461 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 2521 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 2581 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2641 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2701 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2761 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2821 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2881 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2941 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3001 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3061 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3121 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3181 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3241 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3301 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3361 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3421 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 3481 cgcagcgagt cagtgagc //