LOCUS Addgene62443 4775 bp DNA circular UNA 13-APR-2015 DEFINITION MXS_CMV::CreERT2-bGHpA. ACCESSION urn.local...1420795778171.859 VERSION urn.local...1420795778171.859 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers polyA_signal 2636..2860 /label="bGHpA" promoter 83..618 /label="CMV" rep_origin 4019..4607 /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" /label="rep origin" sig_peptide 2988..3056 /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." /label="bla signal peptide" gene 647..2629 /label="CreERT2" gene 2988..3848 /gene="bla" /label="bla gene" misc_RNA 4055..4607 /note="RNAII transcript (complementary strand)" /label="RNA" misc_RNA complement(4058..4165) /note="RNAI transcript" /label="RNAI transcript" -10_signal complement(4174..4179) /note="RNAI promoter (clockwise); GCTACA" /label="RNAI promoter (clockwise); GCTACA -10 signal" -10_signal 2939..2944 /note="bla promoter (counter-clockwise); GAGACA" /label="-10 signal" -10_signal 4040..4045 /note="RNAII promoter (counter-clockwise); CGTAAT" /label="-10 signal" -35_signal 4019..4024 /note="RNAII promoter (counter-clockwise); TTGAGA" /label="-35 signal" -35_signal complement(4196..4201) /note="RNAI promoter (clockwise); TTGAAG" /label="RNAI promoter (clockwise); TTGAAG -35 signal" -35_signal 2918..2923 /note="bla promoter (counter-clockwise); TTCAAA" /label="-35 signal" ORIGIN 1 catgatatca aacgcgtaac tcgagtagtt attaatagta atcaattacg gggtcattag 61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag 601 tgaaccgtca gatccgctag ggatctcagc tggtcgagtc gccaccatgt ccaatttact 661 gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa 721 gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct 781 tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc 841 cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt 901 aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc 961 acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt 1021 tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt 1081 tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg 1141 gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat 1201 ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag 1261 caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat 1321 ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa 1381 tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt 1441 tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc 1501 ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc 1561 aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat 1621 ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgatctgga 1681 gccatctgct ggagacatga gagctgccaa cctttggcca agcccgctca tgatcaaacg 1741 ctctaagaag aacagcctgg ccttgtccct gacggccgac cagatggtca gtgccttgtt 1801 ggatgctgag ccccccatac tctattccga gtatgatcct accagaccct tcagtgaagc 1861 ctcgatgatg ggcttactga ccaacctggc agacagggag ctggttcaca tgatcaactg 1921 ggcgaagagg gtgccaggct ttgtggattt gaccctccat gatcaggtcc accttctaga 1981 atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg cgctccatgg agcacccagt 2041 gaagctactg tttgctccta acttgctctt ggacaggaac cagggaaaat gtgtagaggg 2101 catggtggag atcttcgaca tgctgctggc tacatcatct cggttccgca tgatgaatct 2161 gcagggagag gagtttgtgt gcctcaaatc tattattttg cttaattctg gagtgtacac 2221 atttctgtcc agcaccctga agtctctgga agagaaggac catatccacc gagtcctgga 2281 caagatcaca gacactttga tccacctgat ggccaaggca ggcctgaccc tgcagcagca 2341 gcaccagcgg ctggcccagc tcctcctcat cctctcccac atcaggcaca tgagtaacaa 2401 aggcatggag catctgtaca gcatgaagtg caagaacgtg gtgcccctct atgacctgct 2461 gctggaggcg gcggacgccc accgcctaca tgcgcccact agccgtggag gggcatccgt 2521 ggaggagacg gaccaaagcc acttggccac tgcgggctct acttcatcgc attccttgca 2581 aaagtattac atcacggggg aggcagaggg tttccctgcc acagcttgag tcgagctgtg 2641 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 2701 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 2761 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 2821 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg tcgaccaatt cggggaaatg 2881 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 2941 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3001 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3061 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3121 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3181 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 3241 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3301 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3361 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3421 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3481 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 3541 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 3601 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 3661 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 3721 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 3781 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 3841 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 3901 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 3961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4141 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 4201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 4561 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 4621 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 4681 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 4741 gcagccgaac gaccgagcgc agcgagtcag tgagc //