LOCUS       Addgene62451            9171 bp    DNA     circular UNA 13-APR-2015
DEFINITION  ES-FUCCI.
ACCESSION   urn.local...1420721390042.43
VERSION     urn.local...1420721390042.43
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      1153..1741
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label="rep origin"
     sig_peptide     122..190
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
                     /label="bla signal peptide"
     misc_RNA        1189..1741
                     /note="RNAII transcript (complementary strand)"
                     /label="RNA"
     misc_RNA        complement(1192..1299)
                     /note="RNAI transcript"
                     /label="RNAI transcript"
     -10_signal      complement(1308..1313)
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label="RNAI promoter (clockwise); GCTACA -10 signal"
     -10_signal      73..78
                     /note="bla promoter (counter-clockwise); GAGACA"
                     /label="-10 signal"
     -10_signal      1174..1179
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label="-10 signal"
     -35_signal      complement(1330..1335)
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label="RNAI promoter (clockwise); TTGAAG -35 signal"
     -35_signal      52..57
                     /note="bla promoter (counter-clockwise); TTCAAA"
                     /label="-35 signal"
     -35_signal      1153..1158
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label="-35 signal"
     polyA_signal    complement(<1921..3088)
                     /label="beta-Globin poly A signal"
     polyA_signal    complement(<4653..>4877)
                     /label="bGHpA"
     polyA_signal    <8941..>9165
                     /label="bGHpA"
     promoter        complement(5897..6404)
                     /label="PGK"
     promoter        complement(4140..4647)
                     /label="PGK"
     promoter        7659..7864
                     /label="minPGK"
     misc_feature    complement(<7335..7636)
                     /label="PGKenhancer"
     misc_feature    complement(<7027..7328)
                     /label="PGKenhancer"
     misc_feature    complement(<6719..7020)
                     /label="PGKenhancer"
     misc_feature    complement(<6411..6712)
                     /label="PGKenhancer"
     gene            complement(3100..4122)
                     /label="HygroR"
     gene            122..982
                     /gene="bla"
                     /label="bla gene"
     gene            7879..8594
                     /label="Citrine"
     gene            complement(5172..5882)
                     /label="mCherry"
     gene            8602..8931
                     /label="hGeminin(1-110)"
     gene            complement(4887..5159)
                     /label="hCdt1(30-120)"
ORIGIN      
        1 aattcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
       61 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      121 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      181 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      241 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      301 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      361 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      421 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      481 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      541 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      601 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      661 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      721 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      781 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      841 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      901 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
      961 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1021 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1081 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1201 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1261 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1321 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1381 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1441 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1501 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1561 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1621 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1681 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1741 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1801 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1861 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcc atgagtcgac
     1921 gcagtcgagt tcataagaga agagggacag ctatgactgg gagtagtcag gagaggagga
     1981 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
     2041 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
     2101 ggacagcagg gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc
     2161 agccagcata tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2221 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattccc acacactatt
     2281 gcaatgaaaa taaatttcct ttattaccca gaagtcagat gctcaagggg cttcatgatg
     2341 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
     2401 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttctttgcca
     2461 aaatgatgag acagcacaac aaccagcacg ttgcccagga gctgtaggaa aaagaagaag
     2521 gcatgaacat ggttagcaga ggggcccggt ttggactcag agtattttat cctcatctca
     2581 aacagtgtat atcattgtaa ccataaagag aaaggcagga tgatgaccag ggtgtagttg
     2641 tttctaccaa taagaatatt tccacgccag ccagaattta tatgcagaaa tattctacct
     2701 tatcatttaa ttataacaat tgttctctaa aactgtgctg aagtacaata taatataccc
     2761 tgattgcctt gaaaaaaaag agattagaga aagtacttac aatctgacaa ataaacaaaa
     2821 gtgaatttaa aaattcgtta caaatgcaag ctaaagttta acgaaaaagt tacagaaaat
     2881 gaaaagaaaa taagaggaga caatggttgt caacagagta gaaagtgaaa gaaacaaaat
     2941 tatcatgagg gtccatggtg atacaaggga catcttccca ttctaaacaa caccctgaaa
     3001 actttgcccc ctccatataa catgaatttt acaatagcga aaaagaaaga acaatcaagg
     3061 gtccccaaac tcaccctgaa gttctcagtc tagctcgacc tattcctttg ccctcggacg
     3121 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     3181 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     3241 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     3301 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     3361 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     3421 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     3481 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     3541 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     3601 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     3661 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     3721 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     3781 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     3841 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
     3901 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     3961 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     4021 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     4081 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatggtgg cgactcgacg
     4141 tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg
     4201 tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc
     4261 tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa
     4321 gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca
     4381 tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct
     4441 gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac
     4501 caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc
     4561 cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg
     4621 ggaaaagcgc ctcccctacc cggtagactc gaccatagag cccaccgcat ccccagcatg
     4681 cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat
     4741 agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt
     4801 gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg
     4861 gcaactagaa ggcacagctc gacttagatg gtgtcctggt cctgcgcgga tgtcaggtgg
     4921 ggcggctgac ctgctgccgg ggtggatttc tttatcttct ggcccggaga agggcaggct
     4981 gggatgtctg gggcctcggg ggtactgggg ctggaaacct cgtccaccga cagccgcagt
     5041 ctcctgcggg ccggtggcct ggcctggtcg cgtccggggg cggcgggcgg gcgggcgcgc
     5101 ttgcggctgc cactggtagc ggaggccggg gcgcggagtg cgggcctggc ggggctgggc
     5161 tcgacatctg tagacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
     5221 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
     5281 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
     5341 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
     5401 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
     5461 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
     5521 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
     5581 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg aatgtcggcg
     5641 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
     5701 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
     5761 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
     5821 accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc
     5881 atggtggcga ctcgacgtcg aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga
     5941 cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag gaccttcggg cgcccgcccc
     6001 gcccctgagc ccgcccctga gcccgccccc ggacccaccc cttcccagcc tctgagccca
     6061 gaaagcgaag gagcaaagct gctattggcc gctgccccaa aggcctaccc gcttccattg
     6121 ctcagcggtg ctgtccatct gcacgagact agtgagacgt gctacttcca tttgtcacgt
     6181 cctgcacgac gcgagctgcg gggcgggggg gaacttcctg actaggggag gagtagaagg
     6241 tggcgcgaag gggccaccaa agaacggagc cggttggcgc ctaccggtgg atgtggaatg
     6301 tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca
     6361 tgctccagac tgccttggga aaagcgcctc ccctacccgg tagactcgac gcctacccgc
     6421 ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt
     6481 tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga
     6541 gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat
     6601 gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct
     6661 aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta gactcgacgc
     6721 ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta
     6781 cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta
     6841 ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac
     6901 cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg
     6961 gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga
     7021 ctcgacgcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga gactagtgag
     7081 acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg gggggaactt
     7141 cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg gagccggttg
     7201 gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt
     7261 gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg cctcccctac
     7321 ccggtagact cgacgcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga
     7381 ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg
     7441 gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaacgga
     7501 gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag
     7561 cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc
     7621 tcccctaccc ggtagactcg agttacgcgt aactcgagct ttggggcagc ggccaatagc
     7681 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     7741 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     7801 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     7861 cgacgtcgag tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     7921 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     7981 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     8041 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
     8101 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     8161 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     8221 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     8281 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     8341 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     8401 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     8461 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     8521 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     8581 gacgagctgt acaaggtcga gatgaatccc agtatgaagc agaaacaaga agaaatcaaa
     8641 gagaatataa agaatagttc tgtcccaaga agaactctga agatgattca gccttctgca
     8701 tctggatctc ttgttggaag agaaaatgag ctgtccgcag gcttgtccaa aaggaaacat
     8761 cggaatgacc acttaacatc tacaacttcc agccctgggg ttattgtccc agaatctagt
     8821 gaaaataaaa atcttggagg agtcacccag gagtcatttg atcttatgat taaagaaaat
     8881 ccatcctctc agtattggaa ggaagtggca gaaaaacgga gaaaggcgct gtaagtcgag
     8941 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     9001 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     9061 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     9121 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggtcgag c
//
