LOCUS       pJKR-H-cdaR              4586 bp    DNA     circular      
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             2707..3421
                     /dnas_title="sfGFP"
                     /vntifkey="4"
                     /label=sfGFP
                     /note="E coli codon optimized with JCAT"
     RBS             2681..2706
                     /dnas_title="g10 RBS"
                     /vntifkey="32"
                     /label=g10 RBS
     terminator      3506..3547
                     /dnas_title="bidir terminator"
                     /vntifkey="43"
                     /label=bidir terminator
                     /note="tetA/orfL bidirectional terminator termination of
                     bla transcript is slightly weaker than opposite direction"
     gene            complement(4062..5219)
                     /note="cdaR"
     promoter        complement(3565..4061)
                     /note="PcdaR"
     promoter        2160..2680
                     /note="gudP sdaR operator/promoter/RBS"
     CDS             59..919
                     /dnas_title="AmpR"
                     /vntifkey="4"
                     /label=AmpR
     rep_origin      1067..1734
                     /dnas_title="pUC origin"
                     /vntifkey="33"
                     /label=pUC origin
     terminator      1735..2159
                     /dnas_title="rrnB terminator"
                     /vntifkey="43"
                     /label=rrnB terminator
     terminator      5273..5320
                     /dnas_title="T7 Terminator"
                     /vntifkey="43"
                     /label=T7 Terminator
ORIGIN      
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgggt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1081 aggatcatct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcaggctgt
     1741 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     1801 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1861 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1921 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2041 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2101 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactcA
     2161 TGCTGTTGAT TGACGCCAGT GAGAACCCGG AACCGGAAAC GGAATCAAAT CCGTGGGTCG
     2221 AACAGTGGGG CACGCTGTTG TCCTGATATG TTCAGCGAGC GGTAAATGTC GTTTTAGCGG
     2281 TGCTGAATCG AATCTTTTCA GGCAAATGCC AGTAAAAACT GCTTCATAGC GCGGATTTTT
     2341 ACTGGCGTTT GCCTGGAGTC AAGCGATCCA TTTCATACTC TTCTTTATTT CTTCGTTTTA
     2401 ACCCTTCCTT TCTTGTTCTT GTTTTCATTT CCGTGAAGTG GATTCCACCG TCCAGGGCTA
     2461 ATGCCAAAAT CGGGCCTCAT TGAACGCATT AATGTTGTGT TGTTGCACGG TGAGCCGCTA
     2521 TGGCGCGCTT TTTATACTGC TATTGCCAGA TATAAACACG CGCCGTATTC GGCGAACGAC
     2581 CTATAAAAAC GGCAAAAAAC ACCCTACGTC ACCTCTGATT TCCTGGCGAT GTCGCAGTCC
     2641 AGAGTGAGCG TGGCTAACGC GAATTTTCAG GAGTGCAACA tttaacttta agaaggagat
     2701 atacatatgc gtataggtga agaactgttc accggtgttg ttccgatcct ggttgaactg
     2761 gacggtgacg ttaacggtca caaattctct gttcgtggtg aaggtgaagg tgacgctacc
     2821 aacggtaaac tgaccctgaa attcatctgc accaccggta aactgccggt tccgtggccg
     2881 accctggtta ccaccctgac ctacggtgtt cagtgcttcg ctcgttaccc ggaccacatg
     2941 aaacagcacg acttcttcaa atctgctatg ccggaaggtt acgttcagga acgtaccatc
     3001 tctttcaaag acgacggtac ctacaaaacc cgtgctgaag ttaaattcga aggtgacacc
     3061 ctggttaacc gtatcgaact gaaaggtatc gacttcaaag aagacggtaa catcctgggt
     3121 cacaaactgg aatacaactt caactctcac aacgtttaca tcaccgctga caaacagaaa
     3181 aacggtatca aagctaactt caaaatccgt cacaacgttg aagacggttc tgttcagctg
     3241 gctgaccact accagcagaa caccccgatc ggtgacggtc cggttctgct gccggacaac
     3301 cactacctgt ctacccagtc tgttctgtct aaagacccga acgaaaaacg tgaccacatg
     3361 gttctgctgg aattcgttac cgctgctggt atcacccacg gtatggacga actgtacaaa
     3421 gatgcatgcc agttctagca taaccctaat gagtgagcta acttacatta attgcgttgc
     3481 gccttaatta acggcactcc tcagcaaata taatgaccct cttgataacc caagagggca
     3541 ttttttaatg cccatggcgt ttacCAAACT GACCCTGGGC TTACTGCGCG ACGGTAAGCA
     3601 GGTTAACGTG AACCTGGAAC TGCAGCAGAG CAGCCAGAAT CAGGTTGATT CCAGCTCCAT
     3661 CTTCAACGGC ATTGAAGGCG CTGAGATGAG CAACAAAGGC AAAGATCAGG GCGTGGTAGT
     3721 GAACAACGTG AAAACGGGCA CTCCGGCTGC GCAGATCGGC CTGAAGAAAG GTGATGTGAT
     3781 TATTGGCGCG AACCAGCAGG CAGTGAAAAA CATCGCTGAA CTGCGTAAAG TTCTCGACAG
     3841 CAAACCGTCT GTGCTGGCAC TCAACATTCA GCGCGGCGAC AGCACCATCT ACCTGTTAAT
     3901 GCAGTAATCT CCCTCAACCC CTTCCTGAAA ACGGGAAGGG GTTCTCCTTA CAATCTGTGA
     3961 ACTTCACCAC AACTCCATAC ATCTTCATCA TCCTTTAGGC ATTTGCACAA TGCCGTACGT
     4021 TACGTACTTC CTTATGCTAA GCCGTGCATA ACGGAGGACT TatgGCTGGC TGGCATCTTG
     4081 ATACCAAAAT GGCGCAGGAT ATCGTGGCAC GTACCATGCG CATCATCGAT ACCAATATCA
     4141 ACGTAATGGA TGCCCGTGGG CGAATTATCG GCAGCGGCGA TCGTGAGCGT ATTGGTGAAT
     4201 TGCACGAAGG TGCATTGCTG GTACTTTCAC AGGGACGAGT CGTCGATATC GATGACGCGG
     4261 TAGCACGTCA TCTGCACGGT GTGCGGCAGG GGATTAATCT ACCGTTACGG CTGGAAGGTG
     4321 AAATTGTCGG CGTAATTGGC CTGACAGGTG AACCAGAGAA TCTGCGTAAA TATGGCGAAC
     4381 TGGTCTGCAT GACGGCTGAA ATGATGCTGG AACAGTCGCG GTTGATGCAC TTGTTGGCGC
     4441 AGGATAGCCG TTTGCGGGAA GAACTGGTGA TGAACCTGAT TCAGGCAGAG GAGAATACTC
     4501 CCGCACTTAC TGAATGGGCG CAACGGCTGG GGATCGATCT CAATCAACCG CGAGTGGTGG
     4561 CTATTGTTGA GGTCGACAGC GGTCAGCTTG GCGTGGACAG CGCAATGGCG GAGTTACAAC
     4621 AACTGCAAAA CGCGCTGACT ACGCCCGAGC GTAATAATCT GGTGGCGATT GTCTCGCTAA
     4681 CCGAAATGGT GGTGTTGAAA CCGGCGTTGA ACTCTTTTGG GCGCTGGGAT GCAGAAGATC
     4741 ATCGTAAGCG AGTTGAACAA CTGATTACCC GCATGAAAGA GTACGGCCAG CTGCGTTTTC
     4801 GCGTTTCACT GGGCAACTAT TTTACCGGTC CTAGCAGTAT TGCCCGATCC TATCGTACGG
     4861 CGAAAACGAC GATGGTGGTG GGTAAACAGC GGATGCCAGA AAGTCGCTGC TATTTTTATC
     4921 AGGATCTGAT GTTACCTGTG TTACTCGACA GTTTGCGTGG CGACTGGCAG GCCAACGAAC
     4981 TGGCGCGACC GCTGGCGCGG CTGAAAACGA TGGACAATAA CGGCTTGCTG CGACGAACGC
     5041 TGGCGGCGTG GTTTCGCCAC AATGTGCAAC CGCTGGCAAC GTCAAAGGCG TTGTTTATTC
     5101 ATCGTAATAC CCTGGAGTAT CGGCTTAATC GTATATCGGA ACTGACCGGG CTTGATTTGG
     5161 GCAATTTTGA TGACAGGTTG CTGCTGTATG TGGCGTTACA ACTGGATGAA GAGCGGtagT
     5221 AAgcgactaa aaaattgaat gtaggaaacc aacatgccag ttcgagcaat aactagcata
     5281 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaacctca ggcgagaagc
     5341 acacggtcac act
//

