LOCUS       pJKR-H-mphR              4586 bp    DNA     circular      
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     terminator      1735..2159
                     /dnas_title="rrnB terminator"
                     /vntifkey="43"
                     /label=rrnB terminator
     rep_origin      1067..1734
                     /dnas_title="pUC origin"
                     /vntifkey="33"
                     /label=pUC origin
     CDS             59..919
                     /dnas_title="AmpR"
                     /vntifkey="4"
                     /label=AmpR
     CDS             2260..2971
                     /dnas_title="sfGFP"
                     /vntifkey="4"
                     /label=sfGFP
                     /note="E coli codon optimized with JCAT"
     RBS             2234..2259
                     /dnas_title="g10 RBS"
                     /vntifkey="32"
                     /label=g10 RBS
     terminator      3059..3100
                     /dnas_title="bidir terminator"
                     /vntifkey="43"
                     /label=bidir terminator
                     /note="tetA/orfL bidirectional terminator termination of
                     bla transcript is slightly weaker than opposite direction"
     terminator      5006..5053
                     /dnas_title="T7 Terminator"
                     /vntifkey="43"
                     /label=T7 Terminator
     RBS             3262..3281
                     /note="Strong RBS Hybrid Rinquest SD8 100 and Salis
                     235826"
     misc_feature    3282..3866
                     /note="mphR codon optimized"
     terminator      3875..3980
                     /dnas_title="t0 terminator"
                     /vntifkey="43"
                     /label=t0 terminator
     CDS             complement(4013..4747)
                     /dnas_title="EryR"
                     /vntifkey="4"
                     /label=EryR
                     /note="erythromycin resistance from pGK12-nucMCS"
     misc_feature    2183..2188
                     /note="-35"
     misc_feature    2206..2211
                     /note="-10"
     promoter        2180..2231
                     /note="mphR promoter/operator"
     misc_feature    2218..2218
                     /note="+1 TSS"
     misc_feature    2181..2214
                     /note="mphR operator binding site (DNA footprinting)"
     promoter        3118..3261
                     /note="proA promoter
                     http://nar.oxfordjournals.org/content/39/3/1131.full.pdf"
ORIGIN      
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgggt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1081 aggatcatct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcaggctgt
     1741 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     1801 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1861 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1921 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2041 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2101 gcatcaaatt aagcagaagg ccatccgtca ggatggcctt tttgcgtttc tacaaactct
     2161 gctagcaagt aaggccgacG GATTGAATAT AACCGACGTG ACTGTTACAT TTAGGTGGCT
     2221 AAACCCGTCA ACAtttaact ttaagaagga gatatacata tgcgtaaagg tgaagaactg
     2281 ttcaccggtg ttgttccgat cctggttgaa ctggacggtg acgttaacgg tcacaaattc
     2341 tctgttcgtg gtgaaggtga aggtgacgct accaacggta aactgaccct gaaattcatc
     2401 tgcaccaccg gtaaactgcc ggttccgtgg ccgaccctgg ttaccaccct gacctacggt
     2461 gttcagtgct tcgctcgtta cccggaccac atgaaacagc acgacttctt caaatctgct
     2521 atgccggaag gttacgttca ggaacgtacc atctctttca aagacgacgg tacctacaaa
     2581 acccgtgctg aagttaaatt cgaaggtgac accctggtta accgtatcga actgaaaggt
     2641 atcgacttca aagaagacgg taacatcctg ggtcacaaac tggaatacaa cttcaactct
     2701 cacaacgttt acatcaccgc tgacaaacag aaaaacggta tcaaagctaa cttcaaaatc
     2761 cgtcacaacg ttgaagacgg ttctgttcag ctggctgacc actaccagca gaacaccccg
     2821 atcggtgacg gtccggttct gctgccggac aaccactacc tgtctaccca gtctgttctg
     2881 tctaaagacc cgaacgaaaa acgtgaccac atggttctgc tggaattcgt taccgctgct
     2941 ggtatcaccc acggtatgga cgaactgtac aaagatgcat gccagttcta gcataaccct
     3001 aatgagtgag ctaacttaca ttaattgcgt tgcgccttaa ttaacggcac tcctcagcaa
     3061 atataatgac cctcttgata acccaagagg gcatttttta atgcccatgg cgtttacCAC
     3121 AGCTAACACC ACGTCGTCCC TATCTGCTGC CCTAGGTCTA TGAGTGGTTG CTGGATAACT
     3181 TTACGGGCAT GCATAAGGCT CGtaggctAT ATTCAGGGAG ACCACAACGG TTTCCCTCTA
     3241 CAAATAATTT TGTTTAACTT TGAAATAAGG AGGTAATACA AATGCCGCGT CCGAAACTGA
     3301 AATCTGACGA CGAAGTTCTG GAAGCGGCGA CCGTTGTTCT GAAACGTTGC GGTCCGATCG
     3361 AATTCACCCT GTCTGGTGTT GCGAAAGAAG TTGGTCTGTC TCGTGCGGCG CTGATCCAGC
     3421 GTTTCACCAA CCGTGACACC CTGCTGGTTC GTATGATGGA ACGTGGTGTT GAACAGGTTC
     3481 GTCACTACCT GAACGCGATC CCGATCGGTG CGGGTCCGCA GGGTCTGTGG GAATTCCTGC
     3541 AGGTTCTGGT TCGTTCTATG AACACCCGTA ACGACTTCTC TGTTAACTAC CTGATCTCTT
     3601 GGTACGAACT GCAGGTTCCG GAACTGCGTA CCCTGGCGAT CCAGCGTAAC CGTGCGGTTG
     3661 TTGAAGGTAT CCGTAAACGT CTGCCGCCGG GTGCGCCGGC GGCGGCGGAA CTGCTGCTGC
     3721 ACTCTGTTAT CGCGGGTGCG ACCATGCAGT GGGCGGTTGA CCCGGACGGT GAACTGGCGG
     3781 ACCACGTTCT GGCGCAGATC GCGGCGATCC TGTGCCTGAT GTTCCCGGAA CACGACGACT
     3841 TCCAGCTGCT GCAGCCGCAC GCGTAAtaac tagtgcttgg attctcacca ataaaaaacg
     3901 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     3961 ctatcaacag gagtccaagc gagctcgtaa acttggtctg acagccctta acttacttat
     4021 taaataattt atagctattg aaaagagata agaattgttc aaagctaata ttgtttaaat
     4081 cgtcaattcc tgcatgtttt aaggaattgt taaattgatt ttttgtaaat attttcttgt
     4141 attctttgtt aacccatttc ataacgaaat aattatactt ttgtttatct ttgtgtgata
     4201 ttcttgattt ttttctactt aatctgataa gtgagctatt cactttaggt ttaggatgaa
     4261 aatattctct tggaaccata cttaatatag aaatatcaac ttctgccatt aaaagtaatg
     4321 ccaatgagcg ttttgtattt aataatcttt tagcaaaccc gtattccacg attaaataaa
     4381 tctcattagc tatactatca aaaacaattt tgcgtattat atccgtactt atgttataag
     4441 gtatattacc atatatttta taggattggt ttttaggaaa tttaaactgc aatatatcct
     4501 tgtttaaaac ttggaaatta tcgtgatcaa caagtttatt ttctgtagtt ttgcataatt
     4561 tatggtctat ttcaatggca gttacgaaat tacacctctt tactaattca agggtaaaat
     4621 ggccttttcc tgagccgatt tcaaagatat tatcatgttc atttaatctt atatttgtca
     4681 ttattttatc tatattatgt tttgaagtaa taaagttttg actgtgtttt atatttttct
     4741 cgttcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4861 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4921 ataggcgtat cacgaggccc tttcgtcttc acTAAgcgac taaaaaattg aatgtaggaa
     4981 accaacatgc cagttcgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     5041 gaggggtttt ttgctgaacc tcaggcgaga agcacacggt cacact
//

