LOCUS       pJKR-L-mphR              4586 bp    DNA     circular     
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             47..907
                     /dnas_title="AmpR"
                     /vntifkey="4"
                     /label=AmpR
     CDS             complement(1060..2010)
                     /dnas_title="repA"
                     /vntifkey="4"
                     /label=repA
                     /note="pSC101 repA replication protein"
     rep_origin      complement(2014..2738)
                     /dnas_title="pSC101 ori"
                     /vntifkey="33"
                     /label=pSC101 ori
     terminator      2740..3164
                     /dnas_title="rrnB terminator"
                     /vntifkey="43"
                     /label=rrnB terminator
     CDS             3265..3976
                     /dnas_title="sfGFP"
                     /vntifkey="4"
                     /label=sfGFP
                     /note="E coli codon optimized with JCAT"
     RBS             3239..3264
                     /dnas_title="g10 RBS"
                     /vntifkey="32"
                     /label=g10 RBS
     misc_feature    3188..3193
                     /note="-35"
     misc_feature    3211..3216
                     /note="-10"
     promoter        3185..3236
                     /note="mphR promoter/operator"
     misc_feature    3223..3223
                     /note="+1 TSS"
     misc_feature    3186..3219
                     /note="mphR operator binding site (DNA footprinting)"
     terminator      4064..4105
                     /dnas_title="bidir terminator"
                     /vntifkey="43"
                     /label=bidir terminator
                     /note="tetA/orfL bidirectional terminator termination of
                     bla transcript is slightly weaker than opposite direction"
     RBS             4267..4286
                     /note="Strong RBS Hybrid Rinquest SD8 100 and Salis
                     235826"
     misc_feature    4287..4871
                     /note="mphR codon optimized"
     promoter        4123..4266
                     /note="proA promoter
                     http://nar.oxfordjournals.org/content/39/3/1131.full.pdf"
     terminator      4880..4985
                     /dnas_title="t0 terminator"
                     /vntifkey="43"
                     /label=t0 terminator
     CDS             complement(5018..5752)
                     /dnas_title="EryR"
                     /vntifkey="4"
                     /label=EryR
                     /note="erythromycin resistance from pGK12-nucMCS"
ORIGIN      
        1 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
       61 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      121 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      181 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      241 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      301 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      361 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      421 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      481 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      541 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
      601 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      661 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      721 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
      781 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
      841 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
      901 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
      961 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1021 acgtgagttt tcgttccact gagcgtcaga ccccgaacct cagatccttc cgtatttagc
     1081 cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag
     1141 atcatactta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt
     1201 tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg aatctgatgt
     1261 aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac
     1321 gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg
     1381 cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa
     1441 aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt
     1501 aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt
     1561 taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag
     1621 attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac
     1681 taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc
     1741 tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc
     1801 taatacacca taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt
     1861 gaacgatacc gtccgttctt tccttgtggg gttttcaatc gtggggttga gtagtgccac
     1921 acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc
     1981 atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc
     2041 actataccaa ttgagatggg ctagtcaatg ataattacta gtccttttcc tttgagttgt
     2101 gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc
     2161 tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta
     2221 tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataactc
     2281 actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt ttgctcctct
     2341 acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc
     2401 gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca
     2461 ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc
     2521 ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct
     2581 cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt
     2641 tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga
     2701 ctggctaatg cacccagtaa ggcagcggta tcatcaactg gctgttttgg cggatgagag
     2761 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
     2821 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
     2881 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
     2941 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     3001 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     3061 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca
     3121 gaaggccatc cGTCAggatg gcctttttgc gtttctacaa actctgctag caagtaaggc
     3181 cgacGGATTG AATATAACCG ACGTGACTGT TACATTTAGG TGGCTAAACC CGTCAACAtt
     3241 taactttaag aaggagatat acatatgcgt aaaggtgaag aactgttcac cggtgttgtt
     3301 ccgatcctgg ttgaactgga cggtgacgtt aacggtcaca aattctctgt tcgtggtgaa
     3361 ggtgaaggtg acgctaccaa cggtaaactg accctgaaat tcatctgcac caccggtaaa
     3421 ctgccggttc cgtggccgac cctggttacc accctgacct acggtgttca gtgcttcgct
     3481 cgttacccgg accacatgaa acagcacgac ttcttcaaat ctgctatgcc ggaaggttac
     3541 gttcaggaac gtaccatctc tttcaaagac gacggtacct acaaaacccg tgctgaagtt
     3601 aaattcgaag gtgacaccct ggttaaccgt atcgaactga aaggtatcga cttcaaagaa
     3661 gacggtaaca tcctgggtca caaactggaa tacaacttca actctcacaa cgtttacatc
     3721 accgctgaca aacagaaaaa cggtatcaaa gctaacttca aaatccgtca caacgttgaa
     3781 gacggttctg ttcagctggc tgaccactac cagcagaaca ccccgatcgg tgacggtccg
     3841 gttctgctgc cggacaacca ctacctgtct acccagtctg ttctgtctaa agacccgaac
     3901 gaaaaacgtg accacatggt tctgctggaa ttcgttaccg ctgctggtat cacccacggt
     3961 atggacgaac tgtacaaaga tgcatgccag ttctagcata accctaatga gtgagctaac
     4021 ttacattaat tgcgttgcgc cttaattaac ggcactcctc agcaaatata atgaccctct
     4081 tgataaccca agagggcatt ttttaatgcc catggcgttt acCACAGCTA ACACCACGTC
     4141 GTCCCTATCT GCTGCCCTAG GTCTATGAGT GGTTGCTGGA TAACTTTACG GGCATGCATA
     4201 AGGCTCGtag gctATATTCA GGGAGACCAC AACGGTTTCC CTCTACAAAT AATTTTGTTT
     4261 AACTTTGAAA TAAGGAGGTA ATACAAATGC CGCGTCCGAA ACTGAAATCT GACGACGAAG
     4321 TTCTGGAAGC GGCGACCGTT GTTCTGAAAC GTTGCGGTCC GATCGAATTC ACCCTGTCTG
     4381 GTGTTGCGAA AGAAGTTGGT CTGTCTCGTG CGGCGCTGAT CCAGCGTTTC ACCAACCGTG
     4441 ACACCCTGCT GGTTCGTATG ATGGAACGTG GTGTTGAACA GGTTCGTCAC TACCTGAACG
     4501 CGATCCCGAT CGGTGCGGGT CCGCAGGGTC TGTGGGAATT CCTGCAGGTT CTGGTTCGTT
     4561 CTATGAACAC CCGTAACGAC TTCTCTGTTA ACTACCTGAT CTCTTGGTAC GAACTGCAGG
     4621 TTCCGGAACT GCGTACCCTG GCGATCCAGC GTAACCGTGC GGTTGTTGAA GGTATCCGTA
     4681 AACGTCTGCC GCCGGGTGCG CCGGCGGCGG CGGAACTGCT GCTGCACTCT GTTATCGCGG
     4741 GTGCGACCAT GCAGTGGGCG GTTGACCCGG ACGGTGAACT GGCGGACCAC GTTCTGGCGC
     4801 AGATCGCGGC GATCCTGTGC CTGATGTTCC CGGAACACGA CGACTTCCAG CTGCTGCAGc
     4861 CGCACGCGTA Ataactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     4921 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     4981 caagcgagct cgtaaacttg gtctgacagc ccttaactta cttattaaat aatttatagc
     5041 tattgaaaag agataagaat tgttcaaagc taatattgtt taaatcgtca attcctgcat
     5101 gttttaagga attgttaaat tgattttttg taaatatttt cttgtattct ttgttaaccc
     5161 atttcataac gaaataatta tacttttgtt tatctttgtg tgatattctt gatttttttc
     5221 tacttaatct gataagtgag ctattcactt taggtttagg atgaaaatat tctcttggaa
     5281 ccatacttaa tatagaaata tcaacttctg ccattaaaag taatgccaat gagcgttttg
     5341 tatttaataa tcttttagca aacccgtatt ccacgattaa ataaatctca ttagctatac
     5401 tatcaaaaac aattttgcgt attatatccg tacttatgtt ataaggtata ttaccatata
     5461 ttttatagga ttggttttta ggaaatttaa actgcaatat atccttgttt aaaacttgga
     5521 aattatcgtg atcaacaagt ttattttctg tagttttgca taatttatgg tctatttcaa
     5581 tggcagttac gaaattacac ctctttacta attcaagggt aaaatggcct tttcctgagc
     5641 cgatttcaaa gatattatca tgttcattta atcttatatt tgtcattatt ttatctatat
     5701 tatgttttga agtaataaag ttttgactgt gttttatatt tttctcgttc atACTCTTCC
     5761 TTTTTCAAT
//

