LOCUS pjKR-L-ttgR 4586 bp DNA circular SOURCE ORGANISM FEATURES Location/Qualifiers terminator 2752..3176 /dnas_title="rrnB terminator" /vntifkey="43" /label=rrnB terminator CDS 59..919 /dnas_title="AmpR" /vntifkey="4" /label=AmpR CDS 3276..3989 /dnas_title="sfGFP" /vntifkey="4" /label=sfGFP /note="E coli codon optimized with JCAT" RBS 3250..3275 /dnas_title="g10 RBS" /vntifkey="32" /label=g10 RBS terminator 4075..4116 /dnas_title="bidir terminator" /vntifkey="43" /label=bidir terminator /note="tetA/orfL bidirectional terminator termination of bla transcript is slightly weaker than opposite direction" RBS 4278..4297 /note="Strong RBS Hybrid Rinquest SD8 100 and Salis 235826" promoter 4134..4277 /note="proA promoter" misc_feature 4298..4930 /note="TtgR optimized" terminator 4984..5031 /dnas_title="T7 Terminator" /vntifkey="43" /label=T7 Terminator misc_feature 3201..3239 /note="TtgR binding site" misc_feature 3207..3218 /note="palindrome 1" misc_feature 3223..3234 /note="palindrome 2" misc_feature 3244..3244 /note="TSS" promoter 3197..3247 /note="ttgR promoter" CDS complement(1072..2022) /dnas_title="repA" /vntifkey="4" /label=repA /note="pSC101 repA replication protein" rep_origin complement(2026..2750) /dnas_title="pSC101 ori" /vntifkey="33" /label=pSC101 ori ORIGIN 1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg 781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgaac ctcagatcct 1081 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa 1141 cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct caaaactggt 1201 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tatgtaggta 1261 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc 1321 aagttcggtt actagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt 1381 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact 1441 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat 1501 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg 1561 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt 1621 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc 1681 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa 1741 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct 1801 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta 1861 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt 1921 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact 1981 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag 2041 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt 2101 cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc 2161 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt 2221 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc 2281 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct 2341 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag 2401 ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg agtcgctgtc 2461 tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc 2521 actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg 2581 tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg 2641 ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtgac 2701 aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac tggctgtttt 2761 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg 2821 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac 2881 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg 2941 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3001 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa 3061 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca 3121 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctgct 3181 agcaagtaag gccgacCACC CAGCAGTATT TACAAACAAC CATGAATGTA AGTATATTCC 3241 TTAGCAAcat ttaactttaa gaaggagata tacatatgcg taaaggtgaa gaactgttca 3301 ccggtgttgt tccgatcctg gttgaactgg acggtgacgt taacggtcac aaattctctg 3361 ttcgtggtga aggtgaaggt gacgctacca acggtaaact gaccctgaaa ttcatctgca 3421 ccaccggtaa actgccggtt ccgtggccga ccctggttac caccctgacc tacggtgttc 3481 agtgcttcgc tcgttacccg gaccacatga aacagcacga cttcttcaaa tctgctatgc 3541 cggaaggtta cgttcaggaa cgtaccatct ctttcaaaga cgacggtacc tacaaaaccc 3601 gtgctgaagt taaattcgaa ggtgacaccc tggttaaccg tatcgaactg aaaggtatcg 3661 acttcaaaga agacggtaac atcctgggtc acaaactgga atacaacttc aactctcaca 3721 acgtttacat caccgctgac aaacagaaaa acggtatcaa agctaacttc aaaatccgtc 3781 acaacgttga agacggttct gttcagctgg ctgaccacta ccagcagaac accccgatcg 3841 gtgacggtcc ggttctgctg ccggacaacc actacctgtc tacccagtct gttctgtcta 3901 aagacccgaa cgaaaaacgt gaccacatgg ttctgctgga attcgttacc gctgctggta 3961 tcacccacgg tatggacgaa ctgtacaaag atgcatgcca gttctagcat aaccctaatg 4021 agtgagctaa cttacattaa ttgcgttgcg ccttaattaa cggcactcct cagcaaatat 4081 aatgaccctc ttgataaccc aagagggcat tttttaatgc ccatggcgtt tacCACAGCT 4141 AACACCACGT CGTCCCTATC TGCTGCCCTA GGTCTATGAG TGGTTGCTGG ATAACTTTAC 4201 GGGCATGCAT AAGGCTCGTA GGCTATATTC AGGGAGACCA CAACGGTTTC CCTCTACAAA 4261 TAATTTTGTT TAACTTTGAA ATAAGGAGGT AATACAAATG GTGCGTCGCA CCAAAGAAGA 4321 AGCACAGGAA ACGCGTGCGC AGATTATCGA AGCGGCCGAA CGCGCGTTTT ATAAACGTGG 4381 TGTGGCACGT ACCACGCTGG CAGATATTGC AGAACTGGCA GGTGTTACCC GCGGTGCAAT 4441 CTACTGGCAT TTCAACAATA AAGCCGAACT GGTTCAGGCA CTGCTGGATT CTCTGCACGA 4501 AACGCATGAT CACCTGGCCC GTGCAAGCGA ATCTGAAGAT GAACTGGACC CGCTGGGCTG 4561 CATGCGCAAA CTGCTGCTGC AGGTGTTTAA CGAACTGGTT CTGGATGCAC GTACCCGTCG 4621 CATTAATGAA ATCCTGCATC ACAAATGCGA ATTTACGGAT GATATGTGTG AAATTCGTCA 4681 GCAGCGCCAG AGCGCCGTGC TGGATTGTCA TAAAGGTATC ACCCTGGCAC TGGCAAACGC 4741 AGTTCGTCGC GGTCAGCTGC CGGGTGAACT GGATGTGGAA CGCGCAGCGG TTGCGATGTT 4801 TGCCTATGTG GATGGCCTGA TTGGTCGTTG GCTGCTGCTG CCGGATAGTG TTGACCTGCT 4861 GGGCGATGTG GAAAAATGGG TTGATACCGG TCTGGATATG CTGCGTCTGA GCCCGGCGCT 4921 GCGCAAATAA TAAgcgacta aaaaattgaa tgtaggaaac caacatgcca gttcgagcaa 4981 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaacct 5041 caggcatttg agaagcacac ggtcacact //