LOCUS       pjKR-L-ttgR              4586 bp    DNA     circular      
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     terminator      2752..3176
                     /dnas_title="rrnB terminator"
                     /vntifkey="43"
                     /label=rrnB terminator
     CDS             59..919
                     /dnas_title="AmpR"
                     /vntifkey="4"
                     /label=AmpR
     CDS             3276..3989
                     /dnas_title="sfGFP"
                     /vntifkey="4"
                     /label=sfGFP
                     /note="E coli codon optimized with JCAT"
     RBS             3250..3275
                     /dnas_title="g10 RBS"
                     /vntifkey="32"
                     /label=g10 RBS
     terminator      4075..4116
                     /dnas_title="bidir terminator"
                     /vntifkey="43"
                     /label=bidir terminator
                     /note="tetA/orfL bidirectional terminator termination of
                     bla transcript is slightly weaker than opposite direction"
     RBS             4278..4297
                     /note="Strong RBS Hybrid Rinquest SD8 100 and Salis
                     235826"
     promoter        4134..4277
                     /note="proA promoter"
     misc_feature    4298..4930
                     /note="TtgR optimized"
     terminator      4984..5031
                     /dnas_title="T7 Terminator"
                     /vntifkey="43"
                     /label=T7 Terminator
     misc_feature    3201..3239
                     /note="TtgR binding site"
     misc_feature    3207..3218
                     /note="palindrome 1"
     misc_feature    3223..3234
                     /note="palindrome 2"
     misc_feature    3244..3244
                     /note="TSS"
     promoter        3197..3247
                     /note="ttgR promoter"
     CDS             complement(1072..2022)
                     /dnas_title="repA"
                     /vntifkey="4"
                     /label=repA
                     /note="pSC101 repA replication protein"
     rep_origin      complement(2026..2750)
                     /dnas_title="pSC101 ori"
                     /vntifkey="33"
                     /label=pSC101 ori
ORIGIN      
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgaac ctcagatcct
     1081 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
     1141 cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct caaaactggt
     1201 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tatgtaggta
     1261 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
     1321 aagttcggtt actagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
     1381 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
     1441 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     1501 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     1561 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     1621 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     1681 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     1741 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     1801 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta
     1861 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     1921 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     1981 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     2041 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt
     2101 cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     2161 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     2221 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     2281 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     2341 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     2401 ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg agtcgctgtc
     2461 tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc
     2521 actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg
     2581 tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg
     2641 ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtgac
     2701 aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac tggctgtttt
     2761 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     2821 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
     2881 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
     2941 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     3001 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     3061 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     3121 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctgct
     3181 agcaagtaag gccgacCACC CAGCAGTATT TACAAACAAC CATGAATGTA AGTATATTCC
     3241 TTAGCAAcat ttaactttaa gaaggagata tacatatgcg taaaggtgaa gaactgttca
     3301 ccggtgttgt tccgatcctg gttgaactgg acggtgacgt taacggtcac aaattctctg
     3361 ttcgtggtga aggtgaaggt gacgctacca acggtaaact gaccctgaaa ttcatctgca
     3421 ccaccggtaa actgccggtt ccgtggccga ccctggttac caccctgacc tacggtgttc
     3481 agtgcttcgc tcgttacccg gaccacatga aacagcacga cttcttcaaa tctgctatgc
     3541 cggaaggtta cgttcaggaa cgtaccatct ctttcaaaga cgacggtacc tacaaaaccc
     3601 gtgctgaagt taaattcgaa ggtgacaccc tggttaaccg tatcgaactg aaaggtatcg
     3661 acttcaaaga agacggtaac atcctgggtc acaaactgga atacaacttc aactctcaca
     3721 acgtttacat caccgctgac aaacagaaaa acggtatcaa agctaacttc aaaatccgtc
     3781 acaacgttga agacggttct gttcagctgg ctgaccacta ccagcagaac accccgatcg
     3841 gtgacggtcc ggttctgctg ccggacaacc actacctgtc tacccagtct gttctgtcta
     3901 aagacccgaa cgaaaaacgt gaccacatgg ttctgctgga attcgttacc gctgctggta
     3961 tcacccacgg tatggacgaa ctgtacaaag atgcatgcca gttctagcat aaccctaatg
     4021 agtgagctaa cttacattaa ttgcgttgcg ccttaattaa cggcactcct cagcaaatat
     4081 aatgaccctc ttgataaccc aagagggcat tttttaatgc ccatggcgtt tacCACAGCT
     4141 AACACCACGT CGTCCCTATC TGCTGCCCTA GGTCTATGAG TGGTTGCTGG ATAACTTTAC
     4201 GGGCATGCAT AAGGCTCGTA GGCTATATTC AGGGAGACCA CAACGGTTTC CCTCTACAAA
     4261 TAATTTTGTT TAACTTTGAA ATAAGGAGGT AATACAAATG GTGCGTCGCA CCAAAGAAGA
     4321 AGCACAGGAA ACGCGTGCGC AGATTATCGA AGCGGCCGAA CGCGCGTTTT ATAAACGTGG
     4381 TGTGGCACGT ACCACGCTGG CAGATATTGC AGAACTGGCA GGTGTTACCC GCGGTGCAAT
     4441 CTACTGGCAT TTCAACAATA AAGCCGAACT GGTTCAGGCA CTGCTGGATT CTCTGCACGA
     4501 AACGCATGAT CACCTGGCCC GTGCAAGCGA ATCTGAAGAT GAACTGGACC CGCTGGGCTG
     4561 CATGCGCAAA CTGCTGCTGC AGGTGTTTAA CGAACTGGTT CTGGATGCAC GTACCCGTCG
     4621 CATTAATGAA ATCCTGCATC ACAAATGCGA ATTTACGGAT GATATGTGTG AAATTCGTCA
     4681 GCAGCGCCAG AGCGCCGTGC TGGATTGTCA TAAAGGTATC ACCCTGGCAC TGGCAAACGC
     4741 AGTTCGTCGC GGTCAGCTGC CGGGTGAACT GGATGTGGAA CGCGCAGCGG TTGCGATGTT
     4801 TGCCTATGTG GATGGCCTGA TTGGTCGTTG GCTGCTGCTG CCGGATAGTG TTGACCTGCT
     4861 GGGCGATGTG GAAAAATGGG TTGATACCGG TCTGGATATG CTGCGTCTGA GCCCGGCGCT
     4921 GCGCAAATAA TAAgcgacta aaaaattgaa tgtaggaaac caacatgcca gttcgagcaa
     4981 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaacct
     5041 caggcatttg agaagcacac ggtcacact
//

