LOCUS       pJKR-O-acuR   3829 bp    DNA   circular            
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     CDS             2157..2873
                     /dnas_title="CFP"
                     /vntifkey="4"
                     /label=CFP
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     terminator      1632..2056
                     /dnas_title="rrnB terminator"
                     /vntifkey="43"
                     /label=rrnB terminator
     RBS             2131..2156
                     /dnas_title="g10 RBS"
                     /vntifkey="32"
                     /label=g10 RBS
     promoter        2076..2125
                     /note="acuR promoter operator"
     terminator      2934..2975
                     /dnas_title="bidir terminator"
                     /vntifkey="43"
                     /label=bidir terminator
                     /note="tetA/orfL bidirectional terminator termination of
                     bla transcript is slightly weaker than opposite direction"
     RBS             3137..3156
                     /note="Strong RBS Hybrid Rinquest SD8 100 and Salis
                     235826"
     promoter        2993..3136
                     /note="proD promoter"
     gene            3157..3816
                     /note="AcuR optimized Rhodobacter sphaeroides 2.4.1"
     terminator      3870..3917
                     /dnas_title="T7 Terminator"
                     /vntifkey="43"
                     /label=T7 Terminator
     CDS             complement(70..885)
                     /dnas_title="Kan"
                     /vntifkey="4"
                     /label=Kan
     RBS             complement(891..895)
                     /dnas_title="RBS"
                     /vntifkey="32"
                     /label=RBS
     -35_signal      complement(950..955)
                     /dnas_title="-35 Ap"
                     /vntifkey="48"
                     /label=-35 Ap
     -10_signal      complement(927..933)
                     /dnas_title="-10 Ap"
                     /vntifkey="47"
                     /label=-10 Ap
     rep_origin      complement(993..1628)
                     /dnas_title="ColA ori"
                     /vntifkey="33"
                     /label=ColA ori
ORIGIN      
        1 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
       61 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
      121 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
      181 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
      241 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
      301 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
      361 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
      421 tcgtgattgc gcctgagcga gacgaaatac gcggtcgctg ttaaaaggac aattacaaac
      481 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
      541 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
      601 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
      661 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
      721 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
      781 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
      841 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcatactct tcctttttca
      901 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      961 ttagaaaaat aaacaaatag gcatgcagcg cttggtgtcg ggaatccgta aaggatcttc
     1021 ttgagatcct tttacgatcg tcgtaatctc ctgctctgta aacgaaaaaa ccgcctgggg
     1081 aggcggtttg atcgaaggtt aagtcagttg gggaactgct taacctggta actggcttta
     1141 gtggagcgca gataccaaat actgtccttt cagtgtagcc tctgttaggc caccacttca
     1201 agactctcga tatctaaatc cactaattct cagttaccaa tggctgctgc cagtggcgtt
     1261 ttgtcgtgtc tttccgggtt ggactcaaga tgatagttac cggataaggc gcagcagtcg
     1321 ggctgaacgg ggggttcttg cacacagccc agcttggagc gaactgtcta cacggaacgg
     1381 gacgtggtga tttgggtaaa gcctccacca caacacggac gccgcaggac gggaacagga
     1441 gagcgcaaga gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1501 cgccaccact gatttgagcg tcagatttcg tgatgttcgt caggggggcg gagcctatgg
     1561 aaaaacggct tcgctccggc cttattgtct ctctgctaag tatcctcctg gcatcttcta
     1621 ggacgtttca tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat
     1681 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     1741 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     1801 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     1861 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1921 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc
     1981 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
     2041 gcgtttctac aaactcgcta gcaagtaagg ccgacGCTTC ACAACCGCAC TTGATTTAAT
     2101 AGACCATACC GTCTATTATT TCTGGCCATA tttaacttta agaaggagat atacatatga
     2161 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     2221 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     2281 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
     2341 ctactttgac ttggggtgtt caatgctttt caagataccc agatcatatg aaacggcatg
     2401 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     2461 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     2521 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     2581 aatacaacta tatttcacac aatgtataca tcactgcaga caaacaaaag aatggaatca
     2641 aagctcattt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     2701 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     2761 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     2821 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taactaatga
     2881 gtgagctaac ttacattaat tgcgttgcgc cttaattaac ggcactcctc agcaaatata
     2941 atgaccctct tgataaccca agagggcatt ttttaatgcc catggcgttt acCACAGCTA
     3001 ACACCACGTC GTCCCTATCT GCTGCCCTAG GTCTATGAGT GGTTGCTGGA TAACTTTACG
     3061 GGCATGCATA AGGCTCGTAT AATATATTCA GGGAGACCAC AACGGTTTCC CTCTACAAAT
     3121 AATTTTGTTT AACTTTGAAA TAAGGAGGTA ATACAAATGC CGCTGACCGA CACCCCGCCG
     3181 TCTGTTCCGC AGAAACCGCG TCGTGGTCGT CCGCGTGGTG CTCCGGACGC TTCTCTGGCT
     3241 CACCAGTCTC TGATCCGTGC TGGTCTGGAA CACCTGACCG AAAAAGGTTA CTCTTCTGTT
     3301 GGTGTTGACG AAATCCTGAA AGCTGCTCGT GTTCCGAAAG GTTCTTTCTA CCACTACTTC
     3361 CGTAACAAAG CTGACTTCGG TCTGGCTCTG ATCGAAGCTT ACGACACCTA CTTCGCTCGT
     3421 CTCCTCGACC AGGCGTTCCT GGACGGTTCG CTGGCTCCGC TGGCTCGTCT GCGTCTGTTC
     3481 ACCCGTATGG CTGAAGAAGG TATGGCTCGT CACGGTTTCC GTCGTGGTTG CCTGGTTGGT
     3541 AACCTGGGTC AGGAAATGGG TGCTCTGCCG GACGACTTCC GTGCTGCTCT GATCGGTGTT
     3601 CTGGAAACCT GGCAGCGTCG TACCGCTCAG CTGTTCCGTG AAGCTCAGGC TTGCGGTGAA
     3661 CTGTCTGCTG ACCACGACCC GGACGCTCTG GCTGAAGCTT TCTGGATCGG TTGGGAAGGT
     3721 GCTATCCTGC GTGCTAAACT GGAACTGCGT CCGGACCCGC TGCACTCTTT CACCCGTACC
     3781 TTCGGTCGTC ACTTCGTTAC CCGTACCCAG GAATAATAAg cgactaaaaa attgaatgta
     3841 ggaaaccaac atgccagttc gagcaataac tagcataacc ccttggggcc tctaaacggg
     3901 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acact
//

