LOCUS       pJKR-O-mphR              4586 bp    DNA     circular      
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     terminator      2189..2612
                     /dnas_title="rrnB terminator"
                     /vntifkey="43"
                     /label=rrnB terminator
     RBS             2687..2712
                     /dnas_title="g10 RBS"
                     /vntifkey="32"
                     /label=g10 RBS
     terminator      3484..3525
                     /dnas_title="bidir terminator"
                     /vntifkey="43"
                     /label=bidir terminator
                     /note="tetA/orfL bidirectional terminator termination of
                     bla transcript is slightly weaker than opposite direction"
     terminator      5431..5478
                     /dnas_title="T7 Terminator"
                     /vntifkey="43"
                     /label=T7 Terminator
     rep_origin      37..897
                     /dnas_title="p15a"
                     /vntifkey="33"
                     /label=p15a
     terminator      949..993
                     /dnas_title="rrnBT1 terminator"
                     /vntifkey="43"
                     /label=rrnBT1 terminator
                     /note="rrnB1 transcriptional terminator"
     gene            1035..2045
                     /note="spectinomycin resistance gene"
     CDS             2713..3423
                     /dnas_title="mCherry"
                     /vntifkey="4"
                     /label=mCherry
     -10_signal      2649..2654
     misc_feature    2661..2661
                     /note="transcription initiation site of mph(A)"
     misc_feature    2633..2684
                     /note="mphR promoter/operator"
     promoter        3543..3686
                     /note="proB 12% rrnB P1 Davis et al 2011"
     RBS             3687..3706
                     /note="Strong RBS Hybrid Rinquest SD8 100 and Salis
                     235826"
     misc_feature    3707..4291
                     /note="mphR codon optimized"
     terminator      4300..4405
                     /dnas_title="t0 terminator"
                     /vntifkey="43"
                     /label=t0 terminator
     CDS             complement(4438..5172)
                     /dnas_title="EryR"
                     /vntifkey="4"
                     /label=EryR
                     /note="erythromycin resistance from pGK12-nucMCS"
ORIGIN      
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagacgg atcgcttcat gtggcaggag
       61 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
      121 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
      181 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
      241 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
      301 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
      361 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      421 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      481 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
      601 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
      661 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
      721 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
      781 gaccaaaacg atctcaagaa gatcatctta ttaaggggtc tgacgctcag tggaacgaaa
      841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccagc
      901 ggtatcatca acaggcttac ccgtcttact gtcaagagga catccggtca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttgctgagg agacttaggg accctTCGAA
     1021 CCCCAGAGTC CCGCTTATTT GCCGACTACC TTGGTGATCT CGCCTTTCAC GTAGTGGACA
     1081 AATTCTTCCA ACTGATCTGC GCGCGAGGCC AAGCGATCTT CTTCTTGTCC AAGATAAGCC
     1141 TGTCTAGCTT CAAGTATGAC GGGCTGATAC TGGGCCGGCA GGCGCTCCAT TGCCCAGTCG
     1201 GCAGCGACAT CCTTCGGCGC GATTTTGCCG GTTACTGCGC TGTACCAAAT GCGGGACAAC
     1261 GTAAGCACTA CATTTCGCTC ATCGCCAGCC CAGTCGGGCG GCGAGTTCCA TAGCGTTAAG
     1321 GTTTCATTTA GCGCCTCAAA TAGATCCTGT TCAGGAACCG GATCAAAGAG TTCCTCCGCC
     1381 GCTGGACCTA CCAAGGCAAC GCTATGTTCT CTTGCTTTTG TCAGCAAGAT AGCCAGATCA
     1441 ATGTCGATCG TGGCTGGCTC GAAGATACCT GCAAGAATGT CATTGCGCTG CCATTCTCCA
     1501 AATTGCAGTT CGCGCTTAGC TGGATAACGC CACGGAATGA TGTCGTCGTG CACAACAATG
     1561 GTGACTTCTA CAGCGCGGAG AATCTCGCTC TCTCCAGGGG AAGCCGAAGT TTCCAAAAGG
     1621 TCGTTGATCA AAGCTCGCCG CGTTGTTTCA TCAAGCCTTA CGGTCACCGT AACCAGCAAA
     1681 TCAATATCAC TGTGTGGCTT CAGGCCGCCA TCCACTGCGG AGCCGTACAA ATGTACGGCC
     1741 AGCAACGTCG GTTCGAGATG GCGCTCGATG ACGCCAACTA CCTCTGATAG TTGAGTCGAT
     1801 ACTTCGGCGA TCACCGCTTC CCTCATGATG TTTAACTTTG TTTTAGGGCG ACTGCCCTGC
     1861 TGCGTAACAT CGTTGCTGCT CCATAACATC AAACATCGAC CCACGGCGTA ACGCGCTTGC
     1921 TGCTTGGATG CCCGAGGCAT AGACTGTACC CCAAAAAAAC AGTCATAACA AGCCATGAAA
     1981 ACCGCCACTG CGCCGTTACC ACCGCTGCGT TCGGTCAAGG TTCTGGACCA GTTGCGTGAG
     2041 CGCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCAGATC TTGATCCCCT GCGCCATCAG
     2101 ATCCTTGGCG GCAAGAAAGC CATCCAGTTT ACTTTGCAGG GCTTCCCAAC CTTACCAGAG
     2161 GGCGCCCCAG CTGGCAATTC CGACGTCtgg ctgttttggc ggatgagaga agattttcag
     2221 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
     2281 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
     2341 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
     2401 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2461 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     2521 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
     2581 tgacggatgg cctttttgcg tttctacaaa cttgctagca agtaaggccg acGGATTGAA
     2641 TATAACCGAC GTGACTGTTA CATTTAGGTG GCTAAACCCG TCAACAttta actttaagaa
     2701 ggagatatac atatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2761 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2821 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2881 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2941 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     3001 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3061 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3121 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3181 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     3241 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     3301 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     3361 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3421 taactaatga gtgagctaac ttacattaat tgcgttgcgc cttaattaac ggcactcctc
     3481 agcaaatata atgaccctct tgataaccca agagggcatt ttttaatgcc catggcgttt
     3541 acCACAGCTA ACACCACGTC GTCCCTATCT GCTGCCCTAG GTCTATGAGT GGTTGCTGGA
     3601 TAACtttacg GGCATGCATA AGGCTCGtaa tatATATTCA GGGAGACCAC AACGGTTTCC
     3661 CTCTACAAAT AATTTTGTTT AACTTTGAAA TAAGGAGGTA ATACAAATGC CGCGTCCGAA
     3721 ACTGAAATCT GACGACGAAG TTCTGGAAGC GGCGACCGTT GTTCTGAAAC GTTGCGGTCC
     3781 GATCGAATTC ACCCTGTCTG GTGTTGCGAA AGAAGTTGGT CTGTCTCGTG CGGCGCTGAT
     3841 CCAGCGTTTC ACCAACCGTG ACACCCTGCT GGTTCGTATG ATGGAACGTG GTGTTGAACA
     3901 GGTTCGTCAC TACCTGAACG CGATCCCGAT CGGTGCGGGT CCGCAGGGTC TGTGGGAATT
     3961 CCTGCAGGTT CTGGTTCGTT CTATGAACAC CCGTAACGAC TTCTCTGTTA ACTACCTGAT
     4021 CTCTTGGTAC GAACTGCAGG TTCCGGAACT GCGTACCCTG GCGATCCAGC GTAACCGTGC
     4081 GGTTGTTGAA GGTATCCGTA AACGTCTGCC GCCGGGTGCG CCGGCGGCGG CGGAACTGCT
     4141 GCTGCACTCT GTTATCGCGG GTGCGACCAT GCAGTGGGCG GTTGACCCGG ACGGTGAACT
     4201 GGCGGACCAC GTTCTGGCGC AGATCGCGGC GATCCTGTGC CTGATGTTCC CGGAACACGA
     4261 CGACTTCCAG CTGCTGCAGG CGCACGCGTA Ataactagtg cttggattct caccaataaa
     4321 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     4381 tggatctatc aacaggagtc caagcgagct cgtaaacttg gtctgacagc ccttaactta
     4441 cttattaaat aatttatagc tattgaaaag agataagaat tgttcaaagc taatattgtt
     4501 taaatcgtca attcctgcat gttttaagga attgttaaat tgattttttg taaatatttt
     4561 cttgtattct ttgttaaccc atttcataac gaaataatta tacttttgtt tatctttgtg
     4621 tgatattctt gatttttttc tacttaatct gataagtgag ctattcactt taggtttagg
     4681 atgaaaatat tctcttggaa ccatacttaa tatagaaata tcaacttctg ccattaaaag
     4741 taatgccaat gagcgttttg tatttaataa tcttttagca aacccgtatt ccacgattaa
     4801 ataaatctca ttagctatac tatcaaaaac aattttgcgt attatatccg tacttatgtt
     4861 ataaggtata ttaccatata ttttatagga ttggttttta ggaaatttaa actgcaatat
     4921 atccttgttt aaaacttgga aattatcgtg atcaacaagt ttattttctg tagttttgca
     4981 taatttatgg tctatttcaa tggcagttac gaaattacac ctctttacta attcaagggt
     5041 aaaatggcct tttcctgagc cgatttcaaa gatattatca tgttcattta atcttatatt
     5101 tgtcattatt ttatctatat tatgttttga agtaataaag ttttgactgt gttttatatt
     5161 tttctcgttc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5221 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5281 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     5341 taaaaatagg cgtatcacga ggccctttcg tcttcacTAA gcgactaaaa aattgaatgt
     5401 aggaaaccaa catgccagtt cgagcaataa ctagcataac cccttggggc ctctaaacgg
     5461 gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt cacact
//

