LOCUS       pShuttle-CMV\(Íõº£²¨\2013.12.11)        8360 bp    DNA     circular     11-DEC-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|681162870|
COMMENT     VNTDBDATE|681162870|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pShuttle-CMV (Íõº£²¨ 2013.12.11)|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #11=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 1051 0 0 "")) 
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COMMENT                          "DNA Plasmid 'pShuttle-CMV (\315\365\272\243\262\250 2013.12.11)'" 
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COMMENT                          "Created: 12/11/13 07:54\317\302\316\347" 1) 
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FEATURES             Location/Qualifiers
     LTR             1..103
                     /vntifkey="19"
                     /label=L-ITR
     misc_feature    183..331
                     /vntifkey="21"
                     /label=ES
     promoter        341..933
                     /vntifkey="30"
                     /label=CMV
     misc_feature    complement(1900..1922)
                     /vntifkey="21"
                     /label=Rev\primer
     misc_feature    888..907
                     /vntifkey="21"
                     /label=fwd\primer
     terminator      1902..2129
                     /vntifkey="43"
                     /label=SV40\pA
     misc_feature    2134..4388
                     /vntifkey="21"
                     /label=right\arm\homology
     misc_feature    4436..5319
                     /vntifkey="21"
                     /label=left\arm\homology
     LTR             5320..5422
                     /vntifkey="19"
                     /label=R-ITR
     rep_origin      5626..6293
                     /vntifkey="33"
                     /label=pBR322\Ori
     CDS             7102..7893
                     /vntifkey="4"
                     /label=Kanamycin
     CDS             1131..1847
                     /vntifkey="4"
                     /label=VENUS
     CDS             1056..1118
                     /vntifkey="4"
                     /label=T2A
     CDS             960..1034
                     /vntifkey="4"
                     /label=F2A
BASE COUNT     2067 a      2168 c      2246 g      1879 t 
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt 
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 
      901 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgtcga cgcggccgcg 
      961 gaagcggagt gaaacagact ttgaattttg accttctcaa gttggcggga gacgtggagt 
     1021 ccaaccctgg acctaggcct ggaagcggaa cgcgtggaag cggagagggc agaggaagtc 
     1081 tgctaacatg cggtgacgtc gaggagaatc ctggacctct cgaggccacc atggtgagca 
     1141 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 
     1201 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 
     1261 ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 
     1321 ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact 
     1381 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 
     1441 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 
     1501 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 
     1561 acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg 
     1621 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc 
     1681 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct 
     1741 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 
     1801 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtag taatgagcaa 
     1861 gcttctagat aagatatccg atccaccgga tctagataac tgatcataat cagccatacc 
     1921 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 
     1981 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 
     2041 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     2101 ggtttgtcca aactcatcaa tgtatcttaa cgcggatctg ggcgtggtta agggtgggaa 
     2161 agaatatata aggtgggggt cttatgtagt tttgtatctg ttttgcagca gccgccgccg 
     2221 ccatgagcac caactcgttt gatggaagca ttgtgagctc atatttgaca acgcgcatgc 
     2281 ccccatgggc cggggtgcgt cagaatgtga tgggctccag cattgatggt cgccccgtcc 
     2341 tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc tggaacgccg ttggagactg 
     2401 cagcctccgc cgccgcttca gccgctgcag ccaccgcccg cgggattgtg actgactttg 
     2461 ctttcctgag cccgcttgca agcagtgcag cttcccgttc atccgcccgc gatgacaagt 
     2521 tgacggctct tttggcacaa ttggattctt tgacccggga acttaatgtc gtttctcagc 
     2581 agctgttgga tctgcgccag caggtttctg ccctgaaggc ttcctcccct cccaatgcgg 
     2641 tttaaaacat aaataaaaaa ccagactctg tttggatttg gatcaagcaa gtgtcttgct 
     2701 gtctttattt aggggttttg cgcgcgcggt aggcccggga ccagcggtct cggtcgttga 
     2761 gggtcctgtg tattttttcc aggacgtggt aaaggtgact ctggatgttc agatacatgg 
     2821 gcataagccc gtctctgggg tggaggtagc accactgcag agcttcatgc tgcggggtgg 
     2881 tgttgtagat gatccagtcg tagcaggagc gctgggcgtg gtgcctaaaa atgtctttca 
     2941 gtagcaagct gattgccagg ggcaggccct tggtgtaagt gtttacaaag cggttaagct 
     3001 gggatgggtg catacgtggg gatatgagat gcatcttgga ctgtattttt aggttggcta 
     3061 tgttcccagc catatccctc cggggattca tgttgtgcag aaccaccagc acagtgtatc 
     3121 cggtgcactt gggaaatttg tcatgtagct tagaaggaaa tgcgtggaag aacttggaga 
     3181 cgcccttgtg acctccaaga ttttccatgc attcgtccat aatgatggca atgggcccac 
     3241 gggcggcggc ctgggcgaag atatttctgg gatcactaac gtcatagttg tgttccagga 
     3301 tgagatcgtc ataggccatt tttacaaagc gcgggcggag ggtgccagac tgcggtataa 
     3361 tggttccatc cggcccaggg gcgtagttac cctcacagat ttgcatttcc cacgctttga 
     3421 gttcagatgg ggggatcatg tctacctgcg gggcgatgaa gaaaacggtt tccggggtag 
     3481 gggagatcag ctgggaagaa agcaggttcc tgagcagctg cgacttaccg cagccggtgg 
     3541 gcccgtaaat cacacctatt accggctgca actggtagtt aagagagctg cagctgccgt 
     3601 catccctgag caggggggcc acttcgttaa gcatgtccct gactcgcatg ttttccctga 
     3661 ccaaatccgc cagaaggcgc tcgccgccca gcgatagcag ttcttgcaag gaagcaaagt 
     3721 ttttcaacgg tttgagaccg tccgccgtag gcatgctttt gagcgtttga ccaagcagtt 
     3781 ccaggcggtc ccacagctcg gtcacctgct ctacggcatc tcgatccagc atatctcctc 
     3841 gtttcgcggg ttggggcggc tttcgctgta cggcagtagt cggtgctcgt ccagacgggc 
     3901 cagggtcatg tctttccacg ggcgcagggt cctcgtcagc gtagtctggg tcacggtgaa 
     3961 ggggtgcgct ccgggctgcg cgctggccag ggtgcgcttg aggctggtcc tgctggtgct 
     4021 gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag catttgacca tggtgtcata 
     4081 gtccagcccc tccgcggcgt ggcccttggc gcgcagcttg cccttggagg aggcgccgca 
     4141 cgaggggcag tgcagacttt tgagggcgta gagcttgggc gcgagaaata ccgattccgg 
     4201 ggagtaggca tccgcgccgc aggccccgca gacggtctcg cattccacga gccaggtgag 
     4261 ctctggccgt tcggggtcaa aaaccaggtt tcccccatgc tttttgatgc gtttcttacc 
     4321 tctggtttcc atgagccggt gtccacgctc ggtgacgaaa aggctgtccg tgtccccgta 
     4381 tacagacttg agagggagtt taaacgaatt caatagcttg ttgcatgggc ggcgatataa 
     4441 aatgcaaggt gctgctcaaa aaatcaggca aagcctcgcg caaaaaagaa agcacatcgt 
     4501 agtcatgctc atgcagataa aggcaggtaa gctccggaac caccacagaa aaagacacca 
     4561 tttttctctc aaacatgtct gcgggtttct gcataaacac aaaataaaat aacaaaaaaa 
     4621 catttaaaca ttagaagcct gtcttacaac aggaaaaaca acccttataa gcataagacg 
     4681 gactacggcc atgccggcgt gaccgtaaaa aaactggtca ccgtgattaa aaagcaccac 
     4741 cgacagctcc tcggtcatgt ccggagtcat aatgtaagac tcggtaaaca catcaggttg 
     4801 attcacatcg gtcagtgcta aaaagcgacc gaaatagccc gggggaatac atacccgcag 
     4861 gcgtagagac aacattacag cccccatagg aggtataaca aaattaatag gagagaaaaa 
     4921 cacataaaca cctgaaaaac cctcctgcct aggcaaaata gcaccctccc gctccagaac 
     4981 aacatacagc gcttccacag cggcagccat aacagtcagc cttaccagta aaaaagaaaa 
     5041 cctattaaaa aaacaccact cgacacggca ccagctcaat cagtcacagt gtaaaaaagg 
     5101 gccaagtgca gagcgagtat atataggact aaaaaatgac gtaacggtta aagtccacaa 
     5161 aaaacaccca gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca 
     5221 acttcctcaa atcgtcactt ccgttttccc acgttacgtc acttcccatt ttaagaaaac 
     5281 tacaattccc aacacataca agttactccg ccctaaaacc tacgtcaccc gccccgttcc 
     5341 cacgccccgc gccacgtcac aaactccacc ccctcattat catattggct tcaatccaaa 
     5401 ataaggtata ttattgatga tgttaattaa catgcatgga tccatatgcg gtgtgaaata 
     5461 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact 
     5521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     5581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     5641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     5701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     5761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     5821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     5881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     5941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     6001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     6061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     6121 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     6181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     6241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     6301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     6361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     6421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     6481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     6541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     6601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     6661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     6721 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag ccatgagatt atcaaaaagg 
     6781 atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg 
     6841 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag 
     6901 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc 
     6961 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac 
     7021 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag 
     7081 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 
     7141 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 
     7201 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 
     7261 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 
     7321 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 
     7381 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 
     7441 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 
     7501 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 
     7561 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 
     7621 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 
     7681 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 
     7741 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 
     7801 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 
     7861 atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat 
     7921 cagctcattt tttaaccaat aggccgaaat cggcaccatc ccttataaat caaaagaata 
     7981 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt 
     8041 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc 
     8101 atcaccctaa tcaagttttt tgtggtcgag gtgccgtaaa gcactaaatc ggaaccctaa 
     8161 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg 
     8221 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt 
     8281 aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga 
     8341 tcgaattaat tcttaattaa 
//
