LOCUS       pShuttle-CMV\(Íõº£²¨\2013.12.11)        7629 bp    DNA     circular     11-DEC-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|681162870|
COMMENT     VNTDBDATE|681162870|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pShuttle-CMV (Íõº£²¨ 2013.12.11)|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA Plasmid 'pShuttle-CMV (\315\365\272\243\262\250 2013.12.11)'" 
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FEATURES             Location/Qualifiers
     LTR             1..103
                     /vntifkey="19"
                     /label=L-ITR
     misc_feature    183..331
                     /vntifkey="21"
                     /label=ES
     promoter        341..933
                     /vntifkey="30"
                     /label=CMV
     misc_feature    940..1147
                     /vntifkey="21"
                     /label=MCS
     misc_feature    complement(1169..1191)
                     /vntifkey="21"
                     /label=Rev\primer
     misc_feature    888..907
                     /vntifkey="21"
                     /label=fwd\primer
     terminator      1171..1398
                     /vntifkey="43"
                     /label=SV40\pA
     misc_feature    1403..3657
                     /vntifkey="21"
                     /label=right\arm\homology
     misc_feature    3705..4588
                     /vntifkey="21"
                     /label=left\arm\homology
     LTR             4589..4691
                     /vntifkey="19"
                     /label=R-ITR
     rep_origin      4895..5562
                     /vntifkey="33"
                     /label=pBR322\Ori
     CDS             6371..7162
                     /vntifkey="4"
                     /label=Kanamycin
     misc_feature    960..1034
                     /vntifkey="21"
                     /label=F2A
     misc_feature    1056..1118
                     /vntifkey="21"
                     /label=T2A
BASE COUNT     1893 a      1921 c      2038 g      1777 t 
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt 
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 
      901 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgtcga cgcggccgcg 
      961 gaagcggagt gaaacagact ttgaattttg accttctcaa gttggcggga gacgtggagt 
     1021 ccaaccctgg acctaggcct ggaagcggaa cgcgtggaag cggagagggc agaggaagtc 
     1081 tgctaacatg cggtgacgtc gaggagaatc ctggacctct cgagcctaag cttctagata 
     1141 agatatccga tccaccggat ctagataact gatcataatc agccatacca catttgtaga 
     1201 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 
     1261 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     1321 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     1381 actcatcaat gtatcttaac gcggatctgg gcgtggttaa gggtgggaaa gaatatataa 
     1441 ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag ccgccgccgc catgagcacc 
     1501 aactcgtttg atggaagcat tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc 
     1561 ggggtgcgtc agaatgtgat gggctccagc attgatggtc gccccgtcct gcccgcaaac 
     1621 tctactacct tgacctacga gaccgtgtct ggaacgccgt tggagactgc agcctccgcc 
     1681 gccgcttcag ccgctgcagc caccgcccgc gggattgtga ctgactttgc tttcctgagc 
     1741 ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg atgacaagtt gacggctctt 
     1801 ttggcacaat tggattcttt gacccgggaa cttaatgtcg tttctcagca gctgttggat 
     1861 ctgcgccagc aggtttctgc cctgaaggct tcctcccctc ccaatgcggt ttaaaacata 
     1921 aataaaaaac cagactctgt ttggatttgg atcaagcaag tgtcttgctg tctttattta 
     1981 ggggttttgc gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt 
     2041 attttttcca ggacgtggta aaggtgactc tggatgttca gatacatggg cataagcccg 
     2101 tctctggggt ggaggtagca ccactgcaga gcttcatgct gcggggtggt gttgtagatg 
     2161 atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg 
     2221 attgccaggg gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc 
     2281 atacgtgggg atatgagatg catcttggac tgtattttta ggttggctat gttcccagcc 
     2341 atatccctcc ggggattcat gttgtgcaga accaccagca cagtgtatcc ggtgcacttg 
     2401 ggaaatttgt catgtagctt agaaggaaat gcgtggaaga acttggagac gcccttgtga 
     2461 cctccaagat tttccatgca ttcgtccata atgatggcaa tgggcccacg ggcggcggcc 
     2521 tgggcgaaga tatttctggg atcactaacg tcatagttgt gttccaggat gagatcgtca 
     2581 taggccattt ttacaaagcg cgggcggagg gtgccagact gcggtataat ggttccatcc 
     2641 ggcccagggg cgtagttacc ctcacagatt tgcatttccc acgctttgag ttcagatggg 
     2701 gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg ggagatcagc 
     2761 tgggaagaaa gcaggttcct gagcagctgc gacttaccgc agccggtggg cccgtaaatc 
     2821 acacctatta ccggctgcaa ctggtagtta agagagctgc agctgccgtc atccctgagc 
     2881 aggggggcca cttcgttaag catgtccctg actcgcatgt tttccctgac caaatccgcc 
     2941 agaaggcgct cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt 
     3001 ttgagaccgt ccgccgtagg catgcttttg agcgtttgac caagcagttc caggcggtcc 
     3061 cacagctcgg tcacctgctc tacggcatct cgatccagca tatctcctcg tttcgcgggt 
     3121 tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt 
     3181 ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc 
     3241 cgggctgcgc gctggccagg gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc 
     3301 ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag tccagcccct 
     3361 ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga ggcgccgcac gaggggcagt 
     3421 gcagactttt gagggcgtag agcttgggcg cgagaaatac cgattccggg gagtaggcat 
     3481 ccgcgccgca ggccccgcag acggtctcgc attccacgag ccaggtgagc tctggccgtt 
     3541 cggggtcaaa aaccaggttt cccccatgct ttttgatgcg tttcttacct ctggtttcca 
     3601 tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat acagacttga 
     3661 gagggagttt aaacgaattc aatagcttgt tgcatgggcg gcgatataaa atgcaaggtg 
     3721 ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 
     3781 tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 
     3841 aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 
     3901 tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 
     3961 tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 
     4021 cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 
     4081 tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 
     4141 acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 
     4201 ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 
     4261 cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 
     4321 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 
     4381 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 
     4441 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 
     4501 tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 
     4561 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 
     4621 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 
     4681 tattgatgat gttaattaac atgcatggat ccatatgcgg tgtgaaatac cgcacagatg 
     4741 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     4801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     4861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     4921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     4981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     5041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     5101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     5161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     5221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     5281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     5341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     5401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     5461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     5521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     5581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     5641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     5701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     5761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     5821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     5881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     5941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     6001 tttgcgcaac gttgttgcca ttgctgcagc catgagatta tcaaaaagga tcttcaccta 
     6061 gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag 
     6121 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag 
     6181 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt 
     6241 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt 
     6301 cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg 
     6361 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 
     6421 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 
     6481 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 
     6541 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 
     6601 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 
     6661 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 
     6721 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 
     6781 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 
     6841 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag 
     6901 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 
     6961 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 
     7021 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 
     7081 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 
     7141 tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt 
     7201 ttaaccaata ggccgaaatc ggcaccatcc cttataaatc aaaagaatag accgagatag 
     7261 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 
     7321 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 
     7381 caagtttttt gtggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 
     7441 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 
     7501 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 
     7561 ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt 
     7621 cttaattaa 
//
