LOCUS       ptreCas9mKate2ps        9281 bp ds-DNA     circular     05-MAR-2015
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...1422583945311.29
VERSION     urn.local...1422583945311.29
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      279..922
                     /label=plasmid_rep_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1023..1272
                     /label=TREmod
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1279..1337
                     /label=CMV_min_right
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1417..5553
                     /label=Cas9
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             5557..5610
                     /label=T2A
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             5626..6318
                     /label=mKate2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6337..6456
                     /note="Geneious type: protein_binding"
                     /label=PEST
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6621..6626
                     /label=polyadenylation signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6650..6655
                     /label=polyadenylation signal(1)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      6718..7173
                     /label=f1 single strand DNA origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        7347..7490
                     /label=SV40
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7698..8492
                     /label=Kanamycin_R
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        8849..8869
                     /label=U6promoterStart
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        8870..9100
                     /label=AlignedRegionU6
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rRNA            9101..9113
                     /label=gRNA_Placeholder
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      9209..9215
                     /label=TerminatorPolIII
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rRNA            9133..9208
                     /label=gRNA_Placeholder(1)
                     /ApEinfo_label=gRNA_Placeholder
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9114..9132
                     /label=T1gRNA
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ctagtggatc cgccacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
       61 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
      121 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
      181 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      241 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      301 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      361 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      421 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
      481 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      541 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      601 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      661 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      721 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      781 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      841 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      901 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      961 cgttatcccc tgattctgtg gataaccgta ttaccgcccc gtacacgcca cctcgacata
     1021 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
     1081 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
     1141 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
     1201 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
     1261 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
     1321 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
     1381 gctagtggat ccgcggccgt tactagcctt gccaccatgg acaagaagta ctccattggg
     1441 ctcgatatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg
     1501 agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt
     1561 ggcgccctcc tgttcgactc cggggagacg gccgaagcca cgcggctcaa aagaacagca
     1621 cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat
     1681 gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag
     1741 gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac
     1801 catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag
     1861 gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc
     1921 ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg
     1981 gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc
     2041 aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag
     2101 ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg
     2161 acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa
     2221 gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac
     2281 ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg
     2341 aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac
     2401 caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag
     2461 gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc
     2521 caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag
     2581 ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga
     2641 agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat
     2701 ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata
     2761 ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa
     2821 tcagaagaga ccatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc
     2881 cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt
     2941 cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa
     3001 tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc
     3061 gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagactat
     3121 ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac
     3181 gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac
     3241 aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat
     3301 agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg
     3361 aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat
     3421 gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt
     3481 gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc
     3541 cagaaagcac aagtttctgg ccagggggac agtcttcacg agcacatcgc taatcttgca
     3601 ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc
     3661 aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa
     3721 actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata
     3781 aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat
     3841 gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg
     3901 gacatcaatc ggctctccga ctacgacgtg gatcatatcg tgccccagtc ttttctcaaa
     3961 gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg gaagagtgat
     4021 aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac
     4081 gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg
     4141 tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc
     4201 aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa
     4261 ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtctcaga tttcagaaag
     4321 gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac
     4381 ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt
     4441 gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa
     4501 ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc
     4561 gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa
     4621 acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc
     4681 atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa
     4741 agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc
     4801 aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa
     4861 gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc
     4921 atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa
     4981 gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac
     5041 ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg
     5101 ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct caaagggtct
     5161 cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag
     5221 atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat
     5281 aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac
     5341 attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac
     5401 accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt
     5461 catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac
     5521 agcagggctg accccaagaa gaagaggaag gtggctgagg gcagaggaag tcttctaaca
     5581 tgcggtgacg tggaggagaa tcccggccct tccggagggc ccatggtgag cgagctgatt
     5641 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
     5701 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg
     5761 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     5821 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc
     5881 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     5941 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     6001 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag
     6061 accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg
     6121 ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa acccgctaag
     6181 aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc
     6241 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     6301 agcaaactgg ggcacagagg tggaggaggt tccggatctc acggcttccc tcccgaggtg
     6361 gaggagcagg ccgccggcac cctgcccatg agctgcgccc aggagagcgg catggataga
     6421 caccctgctg cttgcgccag cgccaggatc aacgtctaag gccggccagc ggccgcgact
     6481 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6541 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6601 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6661 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     6721 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     6781 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     6841 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     6901 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     6961 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     7021 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     7081 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     7141 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     7201 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7261 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     7321 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7381 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7621 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     7681 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7741 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7801 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7861 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7921 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7981 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     8041 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     8101 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     8161 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     8221 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     8281 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8341 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8401 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8461 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     8521 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     8581 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8641 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     8701 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     8761 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     8821 gagacctcag tcgactggat ccggtaccaa ggtcgggcag gaagagggcc tatttcccat
     8881 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
     8941 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     9001 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     9061 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgTGAGAAT
     9121 CAAGGCGGTC GAgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     9181 acttgaaaaa gtggcaccga gtcggtgctt tttttctaga cccagctttc ttgtacaaag
     9241 ttggcattaa agggcgaatt ccagcacact ggcggccgtt a 
//
