LOCUS       Exported File           8333 bp ds-DNA    circular SYN 06-FEB-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pJW4.12 pptr-1 in pJP109
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 6, 2015 from SnapGene Viewer 1.1.2
            http://www.snapgene.com
COMMENT     Insert from /Users/jennwang2/Documents/Grad School/Lab/Primary 
            data/Sequences/pJW1 ENTRY VECTORS/pptr-1 in pDONR201 ready for 
            insert.ape 414 to 2072
            Backbone from /Users/jennwang2/Documents/Grad School/Lab/Primary 
            data/Sequences/pJW4 KINASE ASSAYS/pMAL-c2X-gateway destination.gbk 
            4382 to 2699
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..2688
                     /organism="Cloning vector pMAL-c2X"
                     /mol_type="other DNA"
                     /label="source:Cloning vector pMAL-c2X"
                     /note="source:Cloning vector pMAL-c2X"
                     /note="color: #ffc0cb"
     source          4377..8333
                     /organism="Cloning vector pMAL-c2X"
                     /mol_type="other DNA"
                     /label="source:Cloning vector pMAL-c2X(1)"
                     /note="source:Cloning vector pMAL-c2X(1)"
                     /note="color: #ffc0cb"
     gene            15..1163
                     /gene="lacIq"
                     /label="lacIq"
                     /note="lacIq"
                     /note="color: #ffc0cb; direction: RIGHT"
     promoter        15..46
                     /gene="lacIq"
                     /label="lacIq(1)"
                     /note="lacIq lacIq promoter (overexpressing mutant) (c..."
                     /note="lacIq promoter (overexpressing mutant) (clockwise)"
                     /note="color: #ffc0cb"
     CDS             81..1163
                     /codon_start=1
                     /transl_table=11
                     /exception="alternative start codon"
                     /gene="lacIq"
                     /product="lac repressor (LacI)"
                     /label="lac repressor (LacI)"
                     /note="lacIq"
                     /note="color: #ffc0cb"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     gene            1406..2688
                     /gene="malE expression cassette"
                     /label="malE expression cassette"
                     /note="malE expression cassette"
                     /note="color: #ffc0cb; direction: RIGHT"
     promoter        1406..1433
                     /gene="malE expression cassette"
                     /label="malE expression cassette(1)"
                     /note="malE expression cassette Ptac promoter (clockwise)"
                     /note="Ptac promoter (clockwise)"
                     /note="color: #ffc0cb"
     CDS             1528..2688
                     /codon_start=1
                     /gene="malE expression cassette"
                     /product="maltose binding protein (MBP) fusion"
                     /label="maltose binding protein (MBP) fusion"
                     /note="malE expression cassette"
                     /note="malE sequence 1528..2628; multiple cloning site 
                     region 2629..2734; lacZalpha sequence 2735..2916"
                     /note="color: #ffff80"
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGR"
     primer_bind     2587..2610
                     /label="malE primer sequence (NEB #S1237S)"
                     /note="malE primer sequence (NEB #S1237S)"
                     /note="color: #ff8080; direction: RIGHT"
     misc_feature    2587..2608
                     /label="JW234"
                     /note="JW234"
                     /note="color: #00ffff"
     misc_feature    2684..2688
                     /label="XmnI"
                     /note="XmnI"
                     /note="color: #00ffff"
     misc_feature    2689..2699
                     /label="Gateway conversion cassette A"
                     /note="Gateway conversion cassette A"
                     /note="color: #ff80ff"
     misc_feature    2719..4347
                     /label="pptr-1 ORF"
                     /note="pptr-1 ORF"
                     /note="color: #ffff80"
     misc_feature    3208..3210
                     /label="E164"
                     /note="E164"
                     /note="color: #00ffff"
     misc_feature    3267..3287
                     /label="JW32"
                     /note="JW32"
                     /note="color: #00ffff"
     misc_feature    3850..3855
                     /label="SS378"
                     /note="SS378"
                     /note="color: #00ffff"
     misc_feature    3941..3960
                     /label="JW34"
                     /note="JW34"
                     /note="color: #00ffff"
     misc_feature    4182..4204
                     /label="JW35rev"
                     /note="JW35rev"
                     /note="color: #00ffff"
     misc_feature    4359..4376
                     /label="Gateway conversion cassette A(1)"
                     /note="Gateway conversion cassette A(1)"
                     /note="color: #ff80ff"
     gene            4377..4995
                     /gene="malE expression cassette"
                     /label="malE expression cassette(4)"
                     /note="malE expression cassette"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             4377..4604
                     /codon_start=1
                     /gene="malE expression cassette"
                     /product="maltose binding protein (MBP) fusion"
                     /label="maltose binding protein (MBP) fusion(1)"
                     /note="malE expression cassette"
                     /note="malE sequence 1528..2628; multiple cloning site 
                     region 2629..2734; lacZalpha sequence 2735..2916"
                     /note="color: #ffff80"
                     /translation="MSEFGSSRVDLQASLALAVVLQRRDWENPGVTQLNRLAAHPPFAS
                     WRNSEEARTDRPSQQLRSLNGEWQLGCFGG"
     misc_feature    4377..4381
                     /label="XmnI(1)"
                     /note="XmnI(1)"
                     /note="color: #00ffff"
     misc_feature    4383..4420
                     /gene="malE expression cassette"
                     /label="malE expression cassette(2)"
                     /note="malE expression cassette(2)"
                     /note="multiple cloning site (EcoRI-HindIII)"
                     /note="color: #ff8080"
     misc_feature    4404..4421
                     /label="JW235"
                     /note="JW235"
                     /note="color: #00ffff"
     terminator      4792..4995
                     /gene="malE expression cassette"
                     /label="malE expression cassette(3)"
                     /note="malE expression cassette rrnB T1T2 transcriptio..."
                     /note="rrnB T1T2 transcription terminators"
                     /note="color: #ffc0cb"
     gene            5105..5965
                     /gene="bla"
                     /label="bla"
                     /note="bla"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             5105..5965
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="beta-lactamase"
                     /note="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /note="color: #ffc0cb"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     sig_peptide     5105..5173
                     /gene="bla"
                     /label="bla(1)"
                     /note="bla(1)"
                     /note="required for secretion to the periplasm; cleaved off
                     to form the mature beta-lactamase protein"
                     /note="color: #ffc0cb; direction: RIGHT"
     rep_origin      complement(6007..6520)
                     /direction=LEFT
                     /label="M13 origin of replication (+ -)"
                     /note="M13 origin of replication (+ -)"
                     /note="color: #ffc0cb"
     rep_origin      6631..7219
                     /label="pMB1 origin of replication (clockwise) (RNAII -35"
                     /note="pMB1 origin of replication (clockwise) (RNAII -35"
                     /note="pMB1 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point)"
                     /note="color: #ffc0cb"
     gene            complement(7649..7840)
                     /gene="rop"
                     /label="rop"
                     /note="rop"
                     /note="color: #ffc0cb; direction: LEFT"
     CDS             complement(7649..7840)
                     /codon_start=1
                     /transl_table=11
                     /exception="alternative start codon"
                     /gene="rop"
                     /product="ROP protein"
                     /label="ROP protein"
                     /note="rop"
                     /note="color: #ffc0cb"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat cacaagtttg
     2701 tacaaaaaag caggcttaat gcacggaagc gggcacagtc taacgggcgc tccgcatcaa
     2761 attccgccgc cacggacaca gggagccgcc accggtggcc aacaactctc ggcgacagct
     2821 aatcaatttg ttgataaaat cgatccgttc cataataaaa gaggaacttc tagacgattg
     2881 cgcatcaaca acagcagccg atataatgtt gattcggctc aagaactcgt acaattggcg
     2941 ctcatcaaag atactgctgc aaatgagcaa ccggctctcg ttatcgaaaa gctcgtccaa
     3001 tgtcagcacg tattcgattt ctatgatcca gtagctcaac tgaaatgcaa agaaatcaaa
     3061 agagcagctc taaatgagct catcgatcac attacatcga caaaaggagc aattgttgag
     3121 acaatttatc cggctgtcat taaaatggtt gccaagaata tattccgtgt acttccacca
     3181 tcagaaaatt gtgaatttga tccagaagag gatgaaccga ctttagaagt ctcgtggcca
     3241 catttacagc ttgtctacga gctcttcctg agattccttg agtcgccaga tttccaagct
     3301 tcaatcggta aaaagtatat tgatcagaga tttgtgctca agctgctcga tttattcgat
     3361 tcggaggatc cacgtgaacg agactttttg aagacggttc ttcatcgtat ttacggaaaa
     3421 tttttgggcc ttcgggcttt tattcgcaaa catatcaata atatgttttt gagattcgta
     3481 tacgaaacgg actcattcaa cggtgtcggt gagcttctcg agattcttgg ctcaattata
     3541 aacggatttg ctcttccatt gaagcaagag cacaaggttt tccttgtaaa agttcttcta
     3601 ccattgcaca aaccgaaatg cttatcactg tatcatgcac agctcgccta ctgcgtcgtt
     3661 caattcatcg aaaaagactc atcactgact ccacaagttt ttgaggctct gctcaaattt
     3721 tggcctcgaa catgtagcag caaggaggtt atgttcctgg gtgaagtgga ggaaattctc
     3781 gacattatcg aaccggaaca attcaaaaag attatcgatc cattattccg tcaattggcc
     3841 aaatgtgtca gtagtccaca ttttcaagtt gctgaacggg ctctctactt ttggaataat
     3901 gaatatatat tgtcattaat tgaggataca agcagtttgg tgatgccgat tatgttccca
     3961 gcgctctatc ggatttccaa agagcactgg aatcaaacaa ttgttgcact tgtctataat
     4021 gtactcaaaa cttttatgga aatgaatgga aaactgtttg atgagcttac ttctacgtac
     4081 aaaggtgaac gattgcggga aaagcaacga gaaaaggatc gtgatgcttt ttggaagaaa
     4141 atggaagctc ttgaattgaa tccaccggct gaaggaaaag aagtgacacc ttcgttgttt
     4201 ccagagaagt tgactgatta tttgaagaag gacggtccaa atatgacgcc actgccagtt
     4261 gcaactgcag gtggcggtga taaatctcca tctgtagtga agaaatcgtc aacaggatca
     4321 gaaacgacga cgccggctaa aaaataagac ccagctttct tgtacaaagt ggtgatattt
     4381 cagaattcgg atcctctaga gtcgacctgc aggcaagctt ggcactggcc gtcgttttac
     4441 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4501 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4561 gcagcctgaa tggcgaatgg cagcttggct gttttggcgg atgagataag attttcagcc
     4621 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     4681 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     4741 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     4801 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     4861 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     4921 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     4981 acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa
     5041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5281 ccccgaagaa cgtttcccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5341 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6001 tgatttaccc cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat
     6061 atttaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     6121 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     6181 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     6241 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     6301 cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     6361 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     6421 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     6481 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat
     6541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7261 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7321 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7381 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7441 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7501 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     7561 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     7621 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7681 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct
     7741 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     7801 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     7861 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     7921 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     7981 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     8041 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     8101 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     8161 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     8221 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     8281 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgaa att
//
