LOCUS       Exported File           8132 bp ds-DNA    circular SYN 22-APR-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pJW418 MEI-1 T01G9.5b in pJP1.09
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 22, 2015 from SnapGene Viewer 1.1.2
            http://www.snapgene.com
COMMENT     Insert from C:/Users/Jenn Wang/Documents/Grad School/Lab/Primary 
            data/Sequences/pJW1 ENTRY VECTORS/mei-1 T01G9.5b in pDONR201.str 1 
            to 1458
            Backbone from C:/Users/Jenn Wang/Documents/Grad School/Lab/Primary 
            data/Sequences/pJW4 KINASE ASSAYS/pMAL-c2X-gateway destination.gbk 
            4382 to 2699
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..1161
                     /organism="Cloning vector pMAL-c2X"
                     /mol_type="other DNA"
                     /label="source:Cloning vector pMAL-c2X(1)"
                     /note="source:Cloning vector pMAL-c2X(1)"
                     /note="color: #ffc0cb"
     source          2649..6605
                     /organism="Cloning vector pMAL-c2X"
                     /mol_type="other DNA"
                     /label="source:Cloning vector pMAL-c2X(2)"
                     /note="source:Cloning vector pMAL-c2X(2)"
                     /note="color: #ffc0cb"
     source          6606..8132
                     /organism="Cloning vector pMAL-c2X"
                     /mol_type="other DNA"
                     /label="source:Cloning vector pMAL-c2X"
                     /note="source:Cloning vector pMAL-c2X"
                     /note="color: #ffc0cb"
     gene            1..1161
                     /gene="malE expression cassette"
                     /label="malE expression cassette(2)"
                     /note="malE expression cassette"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             1..1161
                     /codon_start=1
                     /gene="malE expression cassette"
                     /product="maltose binding protein (MBP) fusion"
                     /label="maltose binding protein (MBP) fusion(1)"
                     /note="malE expression cassette"
                     /note="malE sequence 1528..2628; multiple cloning site 
                     region 2629..2734; lacZalpha sequence 2735..2916"
                     /note="color: #ffff80"
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGR"
     primer_bind     1060..1083
                     /label="malE primer sequence (NEB #S1237S)"
                     /note="malE primer sequence (NEB #S1237S)"
                     /note="color: #ff8080; direction: RIGHT"
     misc_feature    1157..1161
                     /label="XmnI(1)"
                     /note="XmnI(1)"
                     /note="color: #00ffff"
     misc_feature    1162..1172
                     /label="Gateway conversion cassette A(1)"
                     /note="Gateway conversion cassette A(1)"
                     /note="color: #ff80ff"
     misc_feature    1173..1211
                     /label="JW223"
                     /note="JW223"
                     /note="color: #00ffff"
     misc_feature    1192..2619
                     /label="MEI-1 T01G9.5b cDNA"
                     /note="MEI-1 T01G9.5b cDNA"
                     /note="color: #ffff80"
     misc_feature    2437..2445
                     /label="extra"
                     /note="extra"
                     /note="color: #00ffff"
     misc_feature    2597..2630
                     /label="JW224"
                     /note="JW224"
                     /note="color: #00ffff"
     misc_feature    2631..2648
                     /label="Gateway conversion cassette A"
                     /note="Gateway conversion cassette A"
                     /note="color: #ff80ff"
     gene            2649..3267
                     /gene="malE expression cassette"
                     /label="malE expression cassette(5)"
                     /note="malE expression cassette"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             2649..2876
                     /codon_start=1
                     /gene="malE expression cassette"
                     /product="maltose binding protein (MBP) fusion"
                     /label="maltose binding protein (MBP) fusion"
                     /note="malE expression cassette"
                     /note="malE sequence 1528..2628; multiple cloning site 
                     region 2629..2734; lacZalpha sequence 2735..2916"
                     /note="color: #ffff80"
                     /translation="MSEFGSSRVDLQASLALAVVLQRRDWENPGVTQLNRLAAHPPFAS
                     WRNSEEARTDRPSQQLRSLNGEWQLGCFGG"
     misc_feature    2649..2653
                     /label="XmnI"
                     /note="XmnI"
                     /note="color: #00ffff"
     misc_feature    2655..2692
                     /gene="malE expression cassette"
                     /label="malE expression cassette"
                     /note="malE expression cassette"
                     /note="multiple cloning site (EcoRI-HindIII)"
                     /note="color: #ff8080"
     terminator      3064..3267
                     /gene="malE expression cassette"
                     /label="malE expression cassette(3)"
                     /note="malE expression cassette rrnB T1T2 transcriptio..."
                     /note="rrnB T1T2 transcription terminators"
                     /note="color: #ffc0cb"
     gene            3377..4237
                     /gene="bla"
                     /label="bla"
                     /note="bla"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             3377..4237
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="beta-lactamase"
                     /note="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /note="color: #ffc0cb"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     sig_peptide     3377..3445
                     /gene="bla"
                     /label="bla(1)"
                     /note="bla(1)"
                     /note="required for secretion to the periplasm; cleaved off
                     to form the mature beta-lactamase protein"
                     /note="color: #ffc0cb; direction: RIGHT"
     rep_origin      complement(4279..4792)
                     /direction=LEFT
                     /label="M13 origin of replication (+ -)"
                     /note="M13 origin of replication (+ -)"
                     /note="color: #ffc0cb"
     rep_origin      4903..5491
                     /label="pMB1 origin of replication (clockwise) (RNAII -35"
                     /note="pMB1 origin of replication (clockwise) (RNAII -35"
                     /note="pMB1 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point)"
                     /note="color: #ffc0cb"
     gene            complement(5921..6112)
                     /gene="rop"
                     /label="rop"
                     /note="rop"
                     /note="color: #ffc0cb; direction: LEFT"
     CDS             complement(5921..6112)
                     /codon_start=1
                     /transl_table=11
                     /exception="alternative start codon"
                     /gene="rop"
                     /product="ROP protein"
                     /label="ROP protein"
                     /note="rop"
                     /note="color: #ffc0cb"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     gene            6620..7768
                     /gene="lacIq"
                     /label="lacIq"
                     /note="lacIq"
                     /note="color: #ffc0cb; direction: RIGHT"
     promoter        6620..6651
                     /gene="lacIq"
                     /label="lacIq(1)"
                     /note="lacIq lacIq promoter (overexpressing mutant) (c..."
                     /note="lacIq promoter (overexpressing mutant) (clockwise)"
                     /note="color: #ffc0cb"
     CDS             6686..7768
                     /codon_start=1
                     /transl_table=11
                     /exception="alternative start codon"
                     /gene="lacIq"
                     /product="lac repressor (LacI)"
                     /label="lac repressor (LacI)"
                     /note="lacIq"
                     /note="color: #ffc0cb"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     gene            8011..8132
                     /gene="malE expression cassette"
                     /label="malE expression cassette(1)"
                     /note="malE expression cassette"
                     /note="color: #ffc0cb; direction: RIGHT"
     promoter        8011..8038
                     /gene="malE expression cassette"
                     /label="malE expression cassette(4)"
                     /note="malE expression cassette Ptac promoter (clockwise)"
                     /note="Ptac promoter (clockwise)"
                     /note="color: #ffc0cb"
ORIGIN
        1 atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt
       61 ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat
      121 ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt
      181 atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc
      241 accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac
      301 aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa
      361 gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg
      421 aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg
      481 ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa
      541 gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt
      601 aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa
      661 ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa
      721 gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt
      781 ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc
      841 ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg
      901 ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc
      961 actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc
     1021 tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa
     1081 gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac
     1141 aacctcggga tcgagggaag gatcacaagt ttgtacaaaa aagcaggctt aatgaatggg
     1201 gatgtgcagt cagtcattcg cggatatctg gagcgagccc aagtcgcgaa aacaatgtcc
     1261 gatgccggac gatggaatga ggctggtgat cttcttcgtc agctcatgac agatgtcaaa
     1321 agctgtaaaa tttcggcaag caacagagac gagcatgacg caagaaatac attcctacga
     1381 gccctagagg ctaatttgaa gcttgtccaa caaaatgtgc gcgatgaaga tgacctacac
     1441 gaggcgatga cgcgacaaag tggaagtcca gagccacctg cagacccaga tgtttggtcc
     1501 aaaccatcgc caccgttacc atcctcatcg aaatttggag caacaaagaa aggagttgga
     1561 gcagcaggtc cacgtcccag agaaatatca aagtcaacgt cgtcgatgag cacgaatccg
     1621 gctgatgtga agcctgcgaa tccaacacaa ggaatactgc ctcaaaatag tgctggagat
     1681 tcattcgatg catcggctta tgatgcgtac attgttcaag cggtgcgtgg tacaatggct
     1741 acaaatacgg aaaatacaat gtctttagat gatataatcg gaatgcacga cgtgaagcaa
     1801 gttctgcacg aagccgtcac tcttccactc ttggtgccag aattcttcca gggtctccgt
     1861 tcaccatgga aagcaatggt tctcgctgga ccacctggaa ctggaaagac tcttatcgca
     1921 cgtgcgattg cttcagagtc tagttcaact ttttttaccg tttcctcgac tgatctgtcc
     1981 agcaagtggc gtggtgattc cgagaagatt gttcgtctgt tattcgaact tgcacggttc
     2041 tatgctccat ccataatctt catcgatgag attgacacac ttggtggaca acgtggaaat
     2101 tctggagaac acgaagcgag tcggcgtgtt aaatccgagt ttctggtaca aatggacggg
     2161 tcacagaata aattcgattc gcgacgtgta ttcgtgctgg ccgccacaaa tattccatgg
     2221 gagctcgatg aggcactccg aagacgtttc gaaaagcgaa tcttcattcc actgccagat
     2281 atagatgcaa gaaagaagct tatcgaaaaa tcaatggaag gaactccgaa atccgatgaa
     2341 attaactatg atgacttggc ggcaaggact gaaggattct caggagctga tgttgtgtct
     2401 ctatgcagaa ctgctgctat caatgtgttg agaagatatt tcagatacga caccaaatca
     2461 ttgcgaggcg gggaactgac agctgcaatg gaatcactga aagccgaact cgtgaggaac
     2521 attgactttg aagcagctct tcaagcagtt tcaccaagtg ctggtccaga tacaatgctc
     2581 aaatgcaaag aatggtgtga ctcttttggt gccatgtaag acccagcttt cttgtacaaa
     2641 gtggtgatat ttcagaattc ggatcctcta gagtcgacct gcaggcaagc ttggcactgg
     2701 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2761 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2821 cccaacagtt gcgcagcctg aatggcgaat ggcagcttgg ctgttttggc ggatgagata
     2881 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     2941 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     3001 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     3061 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     3121 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
     3181 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     3241 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
     3301 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3361 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3421 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3481 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3541 tgagagtttt cgccccgaag aacgtttccc aatgatgagc acttttaaag ttctgctatg
     3601 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3661 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3721 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3781 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3841 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3901 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3961 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4021 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4081 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4141 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4201 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4261 tatactttag attgatttac cccggttgat aatcagaaaa gccccaaaaa caggaagatt
     4321 gtataagcaa atatttaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     4381 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4441 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     4501 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     4561 cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     4621 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     4681 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     4741 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtaaaaggat
     4801 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5041 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5581 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5641 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     5701 gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat
     5761 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5821 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5881 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5941 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac
     6001 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     6061 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     6121 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     6181 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     6241 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     6301 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     6361 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     6421 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     6481 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     6541 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg
     6601 aaattccgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga
     6661 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
     6721 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
     6781 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
     6841 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
     6901 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
     6961 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
     7021 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
     7081 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
     7141 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
     7201 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
     7261 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
     7321 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     7381 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     7441 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     7501 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     7561 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     7621 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     7681 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7741 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc
     7801 attaggcaca attctcatgt ttgacagctt atcatcgact gcacggtgca ccaatgcttc
     7861 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcata
     7921 attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa
     7981 cggttctggc aaatattctg aaatgagctg ttgacaatta atcatcggct cgtataatgt
     8041 gtggaattgt gagcggataa caatttcaca caggaaacag ccagtccgtt taggtgtttt
     8101 cacgagcact tcaccaacaa ggaccatagc at
//
