LOCUS       Exported                4835 bp ds-DNA     circular SYN 11-DEC-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 17, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    156..1262
                     /label=1.1 Synapsin P
                     /note="color: #a6acb3"
     regulatory      1277..1286
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             1283..2002
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1283 .. 1285 / #05fd14
                        2: 1286 .. 1288 / #05fd14 / 1a
                        3: 1289 .. 2002 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2009..2089
                     /label=pA SS
                     /note="color: #a6acb3"
     repeat_region   2098..2238
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      2313..2768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        3050..3154
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3155..4015
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3155 .. 3223 / #ccffcc / signal sequence
                        2: 3224 .. 4015 / #ccffcc
                     Cleavage site after base 3223"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4186..4774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgggtt ttggctacgt ccagagcaga
      181 ggaatgaggg catgtagact aaatatgttc gtgtggaaga ggctgaatac acatcagagt
      241 tactgctgca ggaaatgctt ctgcattgca tacccagagt ttccttgctc atctgagagc
      301 atgtgttttt tccagatgtg tgtacttgtg tgagattctc tgggtgtgtg tcaatgtgtt
      361 gcctgaacgt gcattgctca atatgctcat gtgtgttacc ctgggcttgt acatctacat
      421 atatacctgg atgcccgtgt gttctgtgat gtacatatac cctgtgtcat tccttgtttt
      481 tctatttgtg ttattccatg tgttccttca ggctctcact acccaagtgt ccacctccgc
      541 ctgtctggtg atgtttacgc taccccgtgc tcttttcttt gcctgacagt gttgtcgtgg
      601 aagacatctc gccaggaaca ctgcagtaag gagaatttct agttttatgt tcccctccga
      661 gtatgcttct atcccgaccc tcaaccccaa aatgccttca gaggtgaaaa tcaacactgg
      721 aaacacaagt atctgggaag ggtaacaatg caagttagcc tgaggattta ggaggaggct
      781 gaaaaacaga gtaggagcct tactacgggt ccagacccta cggacaagaa cccccactcc
      841 cactccccaa attgcgcatt ccctccccca tcagaggggg aggggaagag gatgcagcgc
      901 ggcgcggcgc gtgcgcactg tcggatttag taccgcggac agagccttcg cccccgctgc
      961 cggcgcgcgc caccacctcc ccagcaccaa aggcgggctg acgtcactct ccagccctcc
     1021 ccaaactccc ctacctcacc gccttggtcg cgtccgtgca gcggtgagtc cagtcgggcc
     1081 gcaccacaag aggtgcaaga taggggggtg caggcgcgac catacgctct gcggcggcag
     1141 agcctcagcg ctgcctcagt ctgcagcggg cagcagagga gtcgcgtcgt gccagagagc
     1201 gccgccgtgc tcctgagccc cttgcgctcc gcccccgcgg cccaccgacc cactgcccct
     1261 tggatccgaa ttcgccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1321 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1381 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1441 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1501 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1561 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1621 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1681 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1741 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1801 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1861 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1921 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1981 catggacgag ctgtacaagt aactcgagtt tattgcagct tataatggtt acaaataaag
     2041 caatagcatc acaaatttca caaataaagc atttttttca ctggaccgag cggccgcagg
     2101 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2161 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     2221 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2281 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     2341 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2521 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2641 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     2761 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     2821 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2881 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2941 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3001 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3061 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3121 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3181 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3241 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3301 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3361 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3421 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3481 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3541 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3601 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3661 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     3721 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3781 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3841 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3901 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3961 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4021 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4081 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4141 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4201 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4261 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4321 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4381 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4441 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4501 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4561 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4621 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4681 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4741 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4801 gttcctggcc ttttgctggc cttttgctca catgt
//
