LOCUS       Exported                5017 bp ds-DNA     circular SYN 18-JUN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 17, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        151..1449
                     /label=1.3 alpha CaMKII Promoter
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      1459..1468
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             1465..2184
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1465 .. 1467 / #05fd14
                        2: 1468 .. 1470 / #05fd14 / 1a
                        3: 1471 .. 2184 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     3'UTR           2191..2272
                     /label=pA SS
                     /note="color: #a6acb3"
     repeat_region   2280..2420
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      2495..2950
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        3232..3336
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3337..4197
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3337 .. 3405 / #ccffcc / signal sequence
                        2: 3406 .. 4197 / #ccffcc
                     Cleavage site after base 3405"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4368..4956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttcgta acattatggc cttaggtcac
      181 ttcatctcca tggggttctt cttctgattt tctagaaaat gagatggggg tgcagagagc
      241 ttcctcagtg acctgcccag ggtcacatca gaaatgtcag agctagaact tgaactcaga
      301 ttactaatct taaattccat gccttggggg catgcaagta cgatatacag aaggagtgaa
      361 ctcattaggg cagatgacca atgagtttag gaaagaagag tccagggcag ggtacatcta
      421 caccacccgc ccagccctgg gtgagtccag ccacgttcac ctcattatag ttgcctctct
      481 ccagtcctac cttgacggga agcacaagca gaaactggga caggagcccc aggagaccaa
      541 atcttcatgg tccctctggg aggatgggtg gggagagctg tggcagaggc ctcaggaggg
      601 gccctgctgc tcagtggtga cagatagggg tgagaaagca gacagagtca ttccgtcagc
      661 attctgggtc tgtttggtac ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg
      721 ctaaaaagca gggagagctg gaaagaaaca aggacagaga cagaggccaa gtcaaccaga
      781 ccaattccca gaggaagcaa agaaaccatt acagagacta caagggggaa gggaaggaga
      841 gatgaattag cttcccctgt aaaccttaga acccagctgt tgccagggca acggggcaat
      901 acctgtctct tcagaggaga tgaagttgcc agggtaacta catcctgtct ttctcaagga
      961 ccatcccaga atgtggcacc cactagccgt taccatagca actgcctctt tgccccactt
     1021 aatcccatcc cgtctgttaa aagggcccta tagttggagg tgggggaggt aggaagagcg
     1081 atgatcactt gtggactaag tttgttcgca tccccttctc caaccccctc agtacatcac
     1141 cctgggggaa cagggtccac ttgctcctgg gcccacacag tcctgcagta ttgtgtatat
     1201 aaggccaggg caaagaggag caggttttaa agtgaaaggc aggcaggtgt tggggaggca
     1261 gttaccgggg caacgggaac agggcgtttc ggaggtggtt gccatgggga cctggatgct
     1321 gacgaaggct cgcgaggctg tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt
     1381 cagtcaagcc ggttctccgt ttgcactcag gagcacgggc aggcgagtgg cccctagttc
     1441 tgggagcagg aattcgccgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1501 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1561 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1621 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1681 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1741 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1801 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1861 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1921 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1981 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2041 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     2101 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2161 ggcatggacg agctgtacaa gtaactcgag tttattgcag cttataatgg ttacaaataa
     2221 agcaatagca tcacaaattt cacaaataaa gcattttttt cactggaccg agcggccgca
     2281 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     2341 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     2401 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2461 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2521 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2581 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2641 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2701 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2761 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2821 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2881 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2941 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3001 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3061 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3121 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3181 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3241 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3301 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3361 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3421 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3481 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3541 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3601 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3661 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3721 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3781 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3841 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3901 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3961 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4021 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4081 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4141 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4201 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4261 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4501 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4741 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4921 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4981 cggttcctgg ccttttgctg gccttttgct cacatgt
//
