LOCUS       pET-Cas9-NLS-6xH        9295 bp ds-DNA     circular     04-FEB-2015
DEFINITION  .
ACCESSION   urn.local...1b-49yt6yk
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    2..29
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            47..63
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    71..73
                     /created_by="User"
                     /Color="#c79810"
                     /modified_by="User"
                     /label="Start ATG"
     misc_feature    74..4174
                     /ApEinfo_fwdcolor="#ff5e69"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="Cas9"
     misc_feature    4175..4195
                     /created_by="User"
                     /label="NLS"
     misc_feature    4196..4204
                     /created_by="User"
                     /label="Met-Asp_Arg linker"
     misc_feature    4205..4228
                     /ApEinfo_fwdcolor="#42d6ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="6x His STOP STOP"
     promoter        complement(5894..5922)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     gene            complement(7043..7234)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     promoter        9279..2
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg
      121 atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa
      181 tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga
      241 gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa
      301 tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt
      361 ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc
      421 tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca
      481 tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc
      541 cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga
      601 taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga
      661 agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag
      721 taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt
      781 atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga
      841 tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa
      901 tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc
      961 agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct
     1021 atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc
     1081 tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa
     1141 cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa
     1201 accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga
     1261 tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg
     1321 tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg
     1381 tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg
     1441 tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa
     1501 ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa
     1561 ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta
     1621 ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc
     1681 agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg
     1741 aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag
     1801 tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt
     1861 gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga
     1921 ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa
     1981 aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac
     2041 tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa
     2101 aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat
     2161 ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg
     2221 cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat
     2281 tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga
     2341 aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc
     2401 gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa
     2461 agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca
     2521 aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga
     2581 tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt
     2641 aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa
     2701 aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt
     2761 tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat
     2821 caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag
     2881 tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac
     2941 cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga
     3001 gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt
     3061 gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga
     3121 tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt
     3181 cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat
     3241 tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg
     3301 gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa
     3361 aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga
     3421 caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc
     3481 aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt
     3541 aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa
     3601 tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa
     3661 actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc
     3721 cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata
     3781 tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt
     3841 gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc
     3901 taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca
     3961 tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa
     4021 tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac
     4081 gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga
     4141 aacacgcatt gatttgagtc agctaggagg tgaccccaag aagaagagga aggtgatgga
     4201 taagcatcac caccaccatc actaatgact cgagtctggt aaagaaaccg ctgctgcgaa
     4261 atttgaacgc cagcacatgg actcgtctac tagcgcagct taattaacct aggctgctgc
     4321 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     4381 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
     4441 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4501 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4561 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4621 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4681 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4741 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4801 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4861 ttaacgttta caatttctgg cggcacgatg gcatgagatt atcaaaaagg atcttcacct
     4921 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4981 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5041 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5101 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5161 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5221 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5281 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5341 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5401 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5461 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5521 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5581 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5641 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5701 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5761 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5821 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatca tgattgaagc
     5881 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5941 caaataggtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6001 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6061 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6781 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6841 acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     6901 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     6961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     7081 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     7141 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     7201 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     7261 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
     7321 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
     7381 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
     7441 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
     7501 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
     7561 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
     7621 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
     7681 gcacgatcat gctagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     7741 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     7801 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7861 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     7921 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     7981 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     8041 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
     8101 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     8161 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     8221 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     8281 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     8341 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     8401 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     8461 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     8521 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     8581 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     8641 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     8701 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     8761 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     8821 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     8881 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     8941 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     9001 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     9061 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     9121 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     9181 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     9241 ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta atacgactca ctata
//
