LOCUS       pET-NLS-Cas9-6xH        9295 bp ds-DNA     circular     04-FEB-2015
DEFINITION  .
ACCESSION   urn.local...16-49yt6uz
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    2..29
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     gene            47..63
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     misc_feature    71..73
                     /created_by="User"
                     /Color="#c79810"
                     /modified_by="User"
                     /label="Start ATG"
     misc_feature    74..79
                     /created_by="User"
                     /modified_by="User"
                     /label="Gly-Ala-linker"
     misc_feature    80..100
                     /created_by="User"
                     /label="NLS"
     misc_feature    101..4204
                     /ApEinfo_fwdcolor="#ff5e69"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Cas9"
     misc_feature    4205..4228
                     /ApEinfo_fwdcolor="#42d6ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="6x His STOP STOP"
     promoter        complement(5894..5922)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     gene            complement(7043..7234)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     promoter        9279..2
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggtgctc ccaagaagaa gaggaaggtg atggataaga aatactcaat
      121 aggcttagat atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt
      181 tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct
      241 tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac
      301 agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc
      361 aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt
      421 ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc
      481 ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga
      541 taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca
      601 ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca
      661 gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga
      721 tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc
      781 tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg
      841 tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc
      901 aaaagatact tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc
      961 tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag
     1021 agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga
     1081 acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta
     1141 taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc
     1201 tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga
     1261 ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa
     1321 cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga
     1381 agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg
     1441 aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg
     1501 gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc
     1561 agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt
     1621 actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt
     1681 caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc
     1741 cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga
     1801 ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt
     1861 taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt
     1921 ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga
     1981 agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt
     2041 gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat
     2101 taatggtatt agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg
     2161 ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga
     2221 cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt
     2281 agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt
     2341 ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa
     2401 tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg
     2461 tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca
     2521 aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga
     2581 attagatatt aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct
     2641 taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc
     2701 ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct
     2761 aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg
     2821 tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat
     2881 cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga
     2941 taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg
     3001 aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc
     3061 gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga
     3121 gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca
     3181 agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa
     3241 aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg
     3301 ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt
     3361 gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa
     3421 ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga
     3481 tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc
     3541 taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac
     3601 aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata
     3661 taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga
     3721 aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc
     3781 tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg
     3841 tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga
     3901 tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt
     3961 agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga
     4021 aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt
     4081 tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct
     4141 tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg
     4201 tgaccatcac caccaccatc actaatgact cgagtctggt aaagaaaccg ctgctgcgaa
     4261 atttgaacgc cagcacatgg actcgtctac tagcgcagct taattaacct aggctgctgc
     4321 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     4381 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
     4441 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4501 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4561 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4621 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4681 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4741 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4801 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4861 ttaacgttta caatttctgg cggcacgatg gcatgagatt atcaaaaagg atcttcacct
     4921 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4981 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5041 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5101 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5161 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5221 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5281 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5341 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5401 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5461 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5521 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5581 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5641 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5701 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5761 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5821 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatca tgattgaagc
     5881 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5941 caaataggtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6001 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6061 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6781 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6841 acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     6901 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     6961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     7081 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     7141 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     7201 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     7261 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
     7321 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
     7381 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
     7441 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
     7501 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
     7561 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
     7621 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
     7681 gcacgatcat gctagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     7741 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     7801 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7861 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     7921 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     7981 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     8041 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
     8101 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     8161 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     8221 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     8281 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     8341 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     8401 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     8461 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     8521 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     8581 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     8641 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     8701 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     8761 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     8821 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     8881 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     8941 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     9001 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     9061 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     9121 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     9181 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     9241 ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta atacgactca ctata
//
