LOCUS       pET-Cre-6xHis           6202 bp ds-DNA     circular     04-FEB-2015
DEFINITION  .
ACCESSION   urn.local...29-47y2tlj
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    2..29
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lacO reg"
                     /Transferred_Similarity="100.00%"
                     /label="lacO reg"
                     /note="Geneious type: regulatory sequence"
     misc_feature    30..35
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     gene            47..63
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     primer_bind     71..73
                     /created_by="User"
                     /Sequence="ATG"
                     /Color="#008800"
                     /modified_by="User"
                     /label="START ATG"
     primer_bind     74..76
                     /Color="#777777"
                     /created_by="User"
                     /label="Ala"
     primer_bind     77..1102
                     /Color="#0077ee"
                     /created_by="User"
                     /label="Cre"
     misc_feature    286..291
                     /Recognition_pattern="TCG^CGA"
                     /annotation_group="TCGCGA"
                     /label="NruI"
                     /note="Geneious type: restriction site"
     misc_feature    529..534
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    623..628
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /label="EcoRV"
                     /note="Geneious type: restriction site"
     misc_feature    879..884
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI"
                     /note="Geneious type: restriction site"
     primer_bind     1103..1111
                     /Color="#777777"
                     /created_by="User"
                     /label="GGS"
     misc_feature    1106..1111
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     primer_bind     1112..1129
                     /Color="#ff9911"
                     /created_by="User"
                     /label="6xHis"
     primer_bind     1130..1132
                     /created_by="User"
                     /Color="#ff3333"
                     /modified_by="User"
                     /label="STOP"
     primer_bind     1133..1135
                     /created_by="User"
                     /Color="#ff3333"
                     /modified_by="User"
                     /label="STOP"
     misc_feature    1136..1141
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    1453..1458
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     misc_feature    1835..1840
                     /Recognition_pattern="TTT^AAA"
                     /annotation_group="TTTAAA"
                     /label="DraI"
                     /note="Geneious type: restriction site"
     misc_feature    1854..1859
                     /Recognition_pattern="TTT^AAA"
                     /annotation_group="TTTAAA"
                     /label="DraI"
                     /note="Geneious type: restriction site"
     misc_feature    2085..2095
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    2338..2343
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    2449..2454
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    2546..2551
                     /Recognition_pattern="TTT^AAA"
                     /annotation_group="TTTAAA"
                     /label="DraI"
                     /note="Geneious type: restriction site"
     misc_feature    2638..2643
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     promoter        complement(2801..2829)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     misc_feature    3262..3267
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     misc_feature    3762..3767
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     gene            complement(3950..4141)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="ROP"
                     /Transferred_Similarity="100.00%"
                     /label="ROP"
     misc_feature    3987..3992
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    4749..4754
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    4842..4847
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    4936..4941
                     /Recognition_pattern="GTT^AAC"
                     /annotation_group="GTTAAC"
                     /label="HpaI"
                     /note="Geneious type: restriction site"
     misc_feature    5258..5264
                     /Recognition_pattern="G^GTNACC"
                     /annotation_group="GGTNACC"
                     /label="BstEII"
                     /note="Geneious type: restriction site"
     misc_feature    5460..5465
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     misc_feature    5969..5974
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI"
                     /note="Geneious type: restriction site"
     misc_feature    6130..6135
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     misc_feature    6164..6169
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI"
                     /note="Geneious type: restriction site"
     promoter        6186..2
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atggcgagca atttactgac cgtacaccaa aatttgcctg cattgccggt
      121 cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc
      181 gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg
      241 caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct
      301 atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa
      361 catgcttcat cgtcggtccg ggctgccacg accaagtgac agcaatgctg tttcactggt
      421 tatgcggcgt atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc
      481 gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg atcgctgcca
      541 ggatatacgt aatctggcat ttctggggat tgcttataac accctgttac gtatagccga
      601 aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca
      661 tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg
      721 ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa
      781 ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct
      841 atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa
      901 ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc
      961 gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac
     1021 caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt
     1081 gcgcctgctg gaagatggcg acggcggatc ccatcaccac caccatcact aatgactcga
     1141 gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact cgtctactag
     1201 cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1261 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
     1321 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1381 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1441 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1501 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1561 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1621 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1681 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1741 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttctggcgg cacgatggca
     1801 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1861 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1921 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1981 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2041 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2101 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2161 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2221 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2281 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2341 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2401 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2461 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2521 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2581 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2641 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2701 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2761 tcttcctttt tcaatcatga ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2821 atatttgaat gtatttagaa aaataaacaa ataggtcatg accaaaatcc cttaacgtga
     2881 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2941 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3001 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3061 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3541 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3601 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3661 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     3721 tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc
     3781 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     3841 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3901 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3961 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     4021 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     4081 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     4141 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     4201 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     4261 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     4321 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     4381 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     4441 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     4501 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
     4561 agcctagccg ggtcctcaac gacaggagca cgatcatgct agtcatgccc cgcgcccacc
     4621 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat
     4681 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4801 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     4861 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     4921 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     4981 cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     5041 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     5101 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     5161 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     5221 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     5281 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     5341 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     5401 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     5461 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     5521 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     5581 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     5641 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     5701 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     5761 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
     5821 tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc
     5881 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     5941 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
     6001 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     6061 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     6121 gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc
     6181 gaaattaata cgactcacta ta
//
