LOCUS       F618+756-699            7713 bp    DNA     circular UNA 05-FEB-2015
DEFINITION  F677.
ACCESSION   urn.local...1370026096244.110
VERSION     urn.local...1370026096244.110
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          order(complement(<5016..7713),complement(1..>3616),
                     complement(<5016..7713))
                     /organism="Cloning vector pRS425"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:31844"
                     /label="source Cloning vector pRS425"
     misc_feature    order(complement(<1..5015),complement(5019..>7713))
                     /note="Geneious type: Concatenated sequence"
                     /label="Ligated sequences 3 (concatenated sequence 1)"
     misc_feature    order(complement(<1..3616),complement(5016..>7713))
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of PRS425 - Fragment 2 extraction
                     (concatenated sequence 2)"
     misc_feature    order(4591..>5015,<3617..3620)
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of pPR3-SUC - Fragment 2 extraction
                     (concatenated sequence 2)"
     misc_feature    complement(3621..>5015)
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of F3+638-628 - Fragment 1 (reversed)
                     (concatenated sequence 1)"
     misc_feature    3621..>5015
                     /note="Geneious type: Concatenated sequence"
                     /label="Ligated sequences 2 (concatenated sequence 1)"
     misc_feature    <3621..4586
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of Ligated sequences - Fragment 1
                     (concatenated sequence 1)"
     promoter        3622..4033
                     /label="pRPR1"
     misc_feature    3622..4585
                     /note="Geneious type: Concatenated sequence"
                     /label="pRH3'+UP-pTEFE2-sgRNA extraction 2 (concatenated
                     sequence 2)"
     misc_feature    3949..4033
                     /note="Geneious type: Leader"
                     /label="RNAse P leader sequence"
     misc_feature    3949
                     /note="Geneious type: TSS"
                     /label="TSS"
     misc_feature    3950..3968
                     /note="Geneious type: bs"
                     /label="TetO"
     misc_feature    4040..4059
                     /note="Geneious type: SDS"
                     /label="a1 spacer"
     misc_feature    4060..4141
                     /note="Geneious type: sgRNA"
                     /label
     terminator      4148..4585
                     /label="RPR1t"
     source          <4586..>4590
                     /organism="pRS316"
                     /mol_type="other DNA"
                     /label="source pRS316"
     misc_feature    <4586..>4590
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of pRS316 (concatenated sequence 1)"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cgggggatct gccaattgaa cataacatgg tagttacata
     3661 tactagtaat atggttcggc acacattaaa agtataaaaa ctatctgaat tacgaattac
     3721 atatattggt cataaaaatc aatcaatcat cgtgtgtttt atatgtctct tatctaagta
     3781 taagaatatc catagttaat attcacttac gctacctttt aacctgtaat cattgtcaac
     3841 aggatatgtt aacgacccac attgataaac gctagtattt ctttttcctc ttcttattgg
     3901 ccggctgtct ctatactccc ctatagtctg tttcttttcg tttcgattgt ccctatcagt
     3961 gatagagatg gcgcacatgg tacgctgtgg tgctcgcggc tgggaacgaa actctgggag
     4021 ctgcgattgg cagaagcttt tactgtatgt acatacagtg ttttagagct agaaatagca
     4081 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     4141 tctcgagcca tatccaactt ccaatttaat ctttcttttt taattttcac ttatttgcga
     4201 tacagaaaga aaaaagcgat agtaactatt gaattttgtt tggatttggt tagattagat
     4261 atggtttctc tttatattta catgctaaaa atgggctaca ccagagatac ataattagat
     4321 atatatacgc cagtacacct tatcggccca agccttgtcc caaggcagcg ttttgttctt
     4381 ggaaacgctg ccctacacgt tcgctatgct tcaagaactt ttctgagcac ttcatgatgc
     4441 atgtttgttc cttattggtt agctttgatg ttgtgaagtc attgacacag tctgtgaaac
     4501 atctttctac cagattagag tacaaacgca tgaaatcctt catttgcttt tgttccacta
     4561 ctttttggaa ctcttgttgt tctttggtac ccaattcgcc ctatagtgag tcgtattacg
     4621 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4681 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4741 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
     4801 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4861 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4921 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4981 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5041 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5101 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5161 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5221 aatattaacg tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5281 tcacaccgca tatcgacggt cgaggagaac ttctagtata tccacatacc taatattatt
     5341 gccttattaa aaatggaatc ccaacaatta catcaaaatc cacattctct tcaaaatcaa
     5401 ttgtcctgta cttccttgtt catgtgtgtt caaaaacgtt atatttatag gataattata
     5461 ctctatttct caacaagtaa ttggttgttt ggccgagcgg tctaaggcgc ctgattcaag
     5521 aaatatcttg accgcagtta actgtgggaa tactcaggta tcgtaagatg caagagttcg
     5581 aatctcttag caaccattat ttttttcctc aacataacga gaacacacag gggcgctatc
     5641 gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc gcctgacgca
     5701 tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagaggcc ggaaccggct
     5761 tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg
     5821 acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt
     5881 ctaacttttc ttacctttta catttcagca atatatatat atatttcaag gatataccat
     5941 tctaatgtct gcccctatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt
     6001 tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc
     6061 caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg
     6121 tgtcccactt ccagatgagg cgctggaagc ctccaagaag gttgatgccg ttttgttagg
     6181 tgctgtggct ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag gtttactaaa
     6241 aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc
     6301 tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt
     6361 cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg gtgatggtgt
     6421 cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc
     6481 tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatct
     6541 tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaattccc
     6601 tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc
     6661 aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga
     6721 agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg cctctttgcc
     6781 agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc cagatttgcc
     6841 aaagaataag gttgacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc
     6901 attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg ttttggatgc
     6961 aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt
     7021 cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat gatatttgta
     7081 cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg
     7141 ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa
     7201 ggaggatagt aaaggaatac aggtaagcaa attgatacta atggctcaac gtgataagga
     7261 aaaagaattg cactttaaca ttaatattga caaggaggag ggcaccacac aaaaagttag
     7321 gtgtaacaga aaatcatgaa actacgattc ctaatttgat attggaggat tttctctaaa
     7381 aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat ggagtttaag
     7441 tcaatacctt cttgaagcat ttcccataat ggtgaaagtt ccctcaagaa ttttactctg
     7501 tcagaaacgg ccttacgacg tagtcgatat ggtgcactct cagtacaatc tgctctgatg
     7561 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7621 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7681 agaggttttc accgtcatca ccgaaacgcg cga
//
