LOCUS       FSE1                    4688 bp    DNA     circular     09-FEB-2015
>8446443.seq - ID: luc99-luc on 2011/9/10-5:28:8 automatically edited with PhredPhrap, start with base no.: 18  Internal Params: Windowsize: 20, Goodqual: 19, Badqual: 10, Minseqlength: 50, nbadelimit: 1
FEATURES             Location/Qualifiers
     misc_feature    218..938
                     /note="CMV"
     misc_feature    2363..2584
                     /note="SV40 polyA+"
     misc_feature    3008..3681
                     /note="REP ORI"
     misc_feature    complement(3828..4688)
                     /note="AMPR"
     misc_feature    983..1002
                     /note="T7"
     misc_feature    1175..1177
                     /note="G"
     misc_feature    1625..2338
                     /note="eGFP VSKGEE"
     misc_feature    1187..1189
                     /note="G"
     misc_feature    1856..1858
                     /note="ATG"
     misc_feature    1886..1888
                     /note="ATG"
     misc_feature    2081..2083
                     /note="ATG"
     misc_feature    complement(1067..1502)
                     /note="5'UTR FMR1"
     misc_feature    1586..1617
                     /note="Peptide PK251  GLY frame   Abs 2E16 and GLY
                     7FM3G666 "
     misc_feature    1507..1621
                     /note="GLY FMR1 ORF"
     misc_feature    1501..1506
                     /note="XHO1"
     misc_feature    1618..1623
                     /note="AVR2"
     misc_feature    1198..1239
                     /note="CGG 14x"
     misc_feature    1241..1494
                     /note="CGG 85x"
     misc_feature    1199..1494
                     /note="GLY 99x"
     misc_feature    2343..2348
                     /note="XBA1"
     misc_feature    1031..1036
                     /note="HIND3"
     misc_feature    1038..1043
                     /note="SAL1"
     misc_feature    1045..1050
                     /note="APA1"
     misc_feature    1052..1057
                     /note="SAC2"
     misc_feature    2598..2603
                     /note="BAMH1"
     misc_feature    2592..2597
                     /note="PML1"
     misc_feature    2586..2591
                     /note="PVU2"
     misc_feature    1160..1162
                     /note="G"
     misc_feature    1059..1066
                     /note="FSE1"
     misc_feature    1168..1173
                     /note="KAS1"
     misc_feature    1163..1165
                     /note="G"
     misc_feature    1167..1169
                     /note="A"
     misc_feature    1138..1140
                     /note="R"
     misc_feature    1109..1111
                     /note="G"
     misc_feature    1073..1075
                     /note="G"
     misc_feature    1157..1166
                     /note="KOZAC RnnrnnACGG"
     source          1..4688
                     /dnas_title="FSE1 GLY99x FMRP eGFP"
ORIGIN      
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      181 tatcacgagg ccctttcgtc ttcaagaatt cagatctTCA ATATTGGCCA TTAGCCATAT
      241 TATTCATTGG TTATATAGCA TAAATCAATA TTGGCTATTG GCCATTGCAT ACGTTGTATC
      301 TATATCATAA TATGTACATT TATATTGGCT CATGTCCAAT ATGACCGCCA TGTTGGCATT
      361 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
      421 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
      481 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
      541 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
      601 ATCATATGCC AAGTCCGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
      661 ATGCCCAGTA CATGACCTTA CGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
      721 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACACCAA TGGGCGTGGA TAGCGGTTTG
      781 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
      841 AAAATCAACG GGACTTTCCA AAATGTCGTA AcAACtCCGC CCCaTTGACG CAAATGGGCG
      901 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCt ctggctaact agagaaccca
      961 ctgcttactg gcttatcgaa atTAATACGA CTCACTATAG GGagacccaa gctgGCTAGC
     1021 gtttaaactt AAGCTTcGTC GACaGGGCCC aCCGCGGaGG CCGGCCTCAG TCAGGCGCTC
     1081 AGCTCCGTTT CGGTTTCACT TCCGGTGGAG GGCCGCCTCT GAGCGGGCGG CGGGCCGACG
     1141 GCGAGCGCGG GCGGCGGCGG TGACGGAGGC GCCGCTGCCA GGGGGCGTGC GGCAGCGCGG
     1201 CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGCGGA GGCGGCGGCG GCGGCGGCGG
     1261 CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG
     1321 CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG
     1381 CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG
     1441 CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCTGGGC
     1501 CTcgagcGCC CGCAGCCCAC CTCTCGGGGG CGGGCTCCCG GCGCTAGCAG GGCTGAAGAG
     1561 AAGATGGAGG AGCTGGTGGT GGAAGTGCGG GGCTCCAATG GCGCTTTCTA CAAGGCACCT
     1621 AggaGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
     1681 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
     1741 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
     1801 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
     1861 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
     1921 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
     1981 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
     2041 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
     2101 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
     2161 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
     2221 CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
     2281 CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGta
     2341 acTCTAGAgc ggccgcttcg agCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC
     2401 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
     2461 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
     2521 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
     2581 GGTAaCAGCT GCACGTGGGA TCCctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2641 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgagcagaca
     2701 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     2761 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     2821 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     2881 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2941 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3001 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3061 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3241 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3481 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3541 ccagttacct tcggaaanag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaanaaaagg atctcaagaa
     3661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagTTA CCAATGCTTA
     3841 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     3901 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     3961 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     4021 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     4081 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     4141 GCTGCAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     4201 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     4261 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     4321 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     4381 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     4441 TCAACACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     4501 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     4561 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     4621 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     4681 ATACTCAT
//

