LOCUS       pGTR        3192 bp    DNA     circular     24-FEB-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|726491724|
COMMENT     VNTDBDATE|726491724|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGTR|
COMMENT     VNTAUTHORNAME|Kabin Xie|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             1515..2375
                     /vntifkey="4"
                     /label=Amp
     misc_feature    complement(61..237)
                     /vntifkey="21"
                     /label=insert
     misc_feature    complement(145..220)
                     /vntifkey="21"
                     /label=gRNA\scaffold
     misc_feature    complement(68..144)
                     /vntifkey="21"
                     /label=tRNA
BASE COUNT      811 a       797 c       804 g       780 t 
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 
       61 tctgccatgc accagccggg aatcgaaccc gggtctgtac cgtggcaggg tactattcta 
      121 ccactagacc actggtgctt tgttgcaccg actcggtgcc actttttcaa gttgataacg 
      181 gactagcctt attttaactt gctatttcta gctctaaaac cgagacctcg gtctccaatc 
      241 actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga 
      301 tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc 
      361 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 
      421 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 
      481 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 
      541 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 
      601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 
      661 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 
      721 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 
      781 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 
      841 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 
      901 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 
      961 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 
     1021 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 
     1081 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 
     1141 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 
     1201 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 
     1261 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 
     1321 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 
     1381 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 
     1441 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 
     1501 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     1561 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     1621 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 
     1681 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     1741 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     1801 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 
     1861 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     1921 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     1981 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     2041 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     2101 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 
     2161 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     2221 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     2281 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     2341 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 
     2401 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     2461 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata 
     2521 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt 
     2581 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 
     2641 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 
     2701 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 
     2761 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 
     2821 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 
     2881 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 
     2941 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 
     3001 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 
     3061 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 
     3121 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata 
     3181 cgactcacta ta 
//
