LOCUS pME-Cas9.txt.xdna 6907 bp DNA linear 2/16/2015 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers misc_feature 744..749 /label=SalI site /SerialCloner_Color=&hFF8080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 756..4934 /label=nls Cas9 nls /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4943..4948 /label=XbaI site /SerialCloner_Color=&hFF8080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 768..788 /label=nls /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4911..4931 /label=nls /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5032..5131 /label=attL2 /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5301..6110 /label=KanR /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 569..668 /label=attL1 /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT 721 TGGGTACCGG GCCCCCCCTC GAGGTCGACG CCACCATGGC TTCTCCACCT AAGAAGAAGA 781 GAAAGGTGGG AAGCATGGAT AAGAAGTATA GCATCGGCCT GGATATTGGA ACTAACTCCG 841 TGGGTTGGGC AGTGATTACA GACGACTACA AGGTCCCTAG CAAGAAATTT AAGGTGCTGG 901 GTAACACCGA CAGGCACAGC ATCAAGAAAA ATCTGATTGG AGCCCTGCTG TTCGGTTCTG 961 GAGAGACTGC CGAAGCAACA CGCCTGAAAA GAACAGCAAG AAGGCGCTAT ACCAGAAGGA 1021 AGAATAGAAT CTGTTACCTG CAGGAGATTT TCTCTAACGA AATGGCTAAG GTGGACGATT 1081 CATTCTTTCA TAGGCTGGAG GAAAGTTTCC TGGTCGAGGA AGATAAGAAA CACGAGCGCC 1141 ATCCTATCTT TGGAAACATT GTGGACGAGG TCGCCTATCA CGAAAAATAC CCAACCATCT 1201 ATCATCTGCG CAAGAAACTG GCTGACTCTA CTGATAAAGC CGACCTGAGA CTGATCTATC 1261 TGGCTCTGGC CCACATGATT AAGTTCAGGG GTCATTTTCT GATCGAGGGC GATCTGAACC 1321 CCGACAATTC CGATGTGGAC AAGCTGTTCA TCCAGCTGGT CCAGATTTAC AATCAGCTGT 1381 TTGAGGAAAA CCCTATTAAT GCTTCCAGAG TGGACGCAAA AGCTATCCTG TCAGCCAGGC 1441 TGTCCAAGTC ACGCAGACTG GAGAACCTGA TTGCACAGCT GCCCGGAGAA AAGAGGAACG 1501 GTCTGTTTGG AAATCTGATC GCTCTGAGTC TGGGCCTGAC TCCTAACTTC AAAAGCAATT 1561 TTGATCTGGC TGAGGACGCC AAACTGCAGC TGTCAAAGGA CACATATGAC GATGACCTGG 1621 ATAACCTGCT GGCACAGATC GGAGATCAGT ACGCTGACCT GTTCCTGGCT GCCAAAAATC 1681 TGTCCGACGC AATCCTGCTG TCAGATATTC TGAGAGTGAA CAGCGAGATT ACAAAAGCAC 1741 CTCTGAGTGC CAGCATGATC AAGAGATATG ACGAGCACCA TCAGGATCTG ACCCTGCTGA 1801 AGGCTCTGGT CAGGCAGCAG CTGCCAGAGA AGTACAAGGA AATTTTCTTT GATCAGTCCA 1861 AGAACGGCTA CGCCGGTTAT ATCGACGGAG GCGCATCACA GGAGGAATTC TACAAGTTTA 1921 TCAAACCTAT TCTGGAGAAG ATGGACGGAA CTGAGGAACT GCTGGTGAAA CTGAATAGAG 1981 AGGACCTGCT GAGGAAGCAG CGCACATTTG ATAACGGTTC CATCCCACAC CAGATTCATC 2041 TGGGAGAGCT GCACGCTATC CTGAGGCGCC AGGAAGACTT CTACCCCTTT CTGAAAGATA 2101 ACCGCGAGAA GATCGAAAAA ATTCTGACCT TCAGAATCCC TTACTATGTG GGTCCACTGG 2161 CTCGCGGAAA CAGCAGATTT GCCTGGATGA CTCGCAAATC CGAGGAAACC ATTACTCCTT 2221 GGAACTTCGA GGAAGTGGTC GATAAGGGCG CCTCTGCACA GTCCTTCATC GAGAGAATGA 2281 CTAATTTTGA CAAAAACCTG CCCAATGAGA AAGTGCTGCC TAAGCACTCC CTGCTGTACG 2341 AGTATTTCAC TGTCTATAAC GAACTGACAA AGGTGAAATA CGTCACCGAG GGCATGAGAA 2401 AGCCAGCCTT CCTGTCAGGA GAGCAGAAGA AAGCAATCGT GGATCTGCTG TTTAAAACCA 2461 ACAGGAAAGT GACTGTCAAG CAGCTGAAGG AGGACTACTT CAAGAAAATT GAATGCTTCG 2521 ATTCCGTGGA GATCAGCGGA GTCGAAGACA GATTTAACGC AAGCCTGGGC GCTTACCACG 2581 ATCTGCTGAA GATCATTAAG GATAAAGACT TCCTGGACAA CGAGGAAAAT GAGGATATCC 2641 TGGAAGACAT TGTGCTGACA CTGACCCTGT TTGAGGACAG AGGAATGATC GAGGAAAGAC 2701 TGAAAACCTA TGCTCATCTG TTCGATGACA AGGTGATGAA ACAGCTGAAG AGAAGGCGCT 2761 ACACTGGCTG GGGTAGACTG AGCAGGAAGC TGATCAACGG CATTAGGGAT AAACAGTCAG 2821 GAAAGACAAT CCTGGACTTT CTGAAAAGTG ATGGCTTCGC CAACCGCAAT TTTATGCAGC 2881 TGATTCACGA TGACAGTCTG ACCTTCAAAG AGGACATCCA GAAGGCTCAG GTGTCTGGAC 2941 AGGGCCACTC CCTGCATGAG CAGATTGCAA ACCTGGCTGG AAGCCCAGCC ATCAAGAAAG 3001 GCATTCTGCA GACAGTGAAA ATCGTCGATG AGCTGGTGAA AGTCATGGGC CATAAGCCCG 3061 AAAACATCGT GATTGAGATG GCTCGCGAAA ATCAGACAAC CCAGAAGGGT CAGAAGAACA 3121 GTAGAGAGAG GATGAAAAGA ATCGAGGAAG GCATTAAGGA GCTGGGTAGC CAGATCCTGA 3181 AAGAGCACCC AGTGGAAAAC ACACAGCTGC AGAATGAGAA GCTGTATCTG TACTATCTGC 3241 AGAATGGAAG AGATATGTAC GTGGACCAGG AGCTGGATAT TAACAGGCTG TCTGATTACG 3301 ACGTGGATCA TATCGTCCCC CAGAGTTTCA TCAAAGATGA CAGCATTGAC AACAAGGTGC 3361 TGACCAGGTC CGACAAAAAC AGAGGAAAAT CAGATAATGT CCCTAGTGAG GAAGTGGTCA 3421 AGAAAATGAA GAACTACTGG AGACAGCTGC TGAATGCCAA ACTGATCACT CAGAGGAAGT 3481 TTGATAACCT GACAAAAGCA GAGCGCGGTG GACTGTCAGA ACTGGACAAA GCTGGATTCA 3541 TCAAGAGGCA GCTGGTGGAA ACACGCCAGA TCACTAAACA CGTCGCACAG ATTCTGGATA 3601 GTCGCATGAA CACAAAGTAC GATGAGAATG ACAAACTGAT CAGAGAAGTG AAGGTCATTA 3661 CCCTGAAGAG TAAACTGGTC AGCGACTTTA GGAAAGATTT CCAGTTTTAT AAGGTCCGCG 3721 AGATTAACAA TTATCACCAT GCCCATGACG CATACCTGAA CGCCGTGGTC GGTACCGCAC 3781 TGATCAAGAA ATACCCAAAA CTGGAGAGCG AATTCGTGTA CGGAGACTAT AAGGTGTACG 3841 ATGTCAGAAA AATGATCGCC AAGTCCGAGC AGGAAATTGG AAAAGCTACT GCCAAGTATT 3901 TCTTTTACTC AAACATCATG AATTTCTTTA AGACAGAGAT CACCCTGGCC AATGGAGAAA 3961 TCCGCAAAAG GCCCCTGATT GAGACAAACG GAGAGACAGG CGAAATCGTG TGGGACAAAG 4021 GCAGAGATTT TGCAACCGTG AGGAAGGTCC TGAGCATGCC TCAAGTGAAT ATCGTCAAGA 4081 AAACTGAGGT GCAGACAGGC GGTTTCTCAA AAGAAAGTAT TCTGCCAAAA CGCAACTCTG 4141 ATAAGCTGAT CGCTAGAAAG AAAGACTGGG ACCCTAAGAA GTATGGAGGC TTTGACTCTC 4201 CCACTGTGGC ATACTCCGTC CTGGTGGTCG CTAAGGTGGA GAAGGGCAAA AGCAAGAAAC 4261 TGAAATCTGT CAAGGAGCTG CTGGGTATCA CAATTATGGA GAGAAGCTCT TTCGAGAAGA 4321 ACCCAATCGA TTTTCTGGAG GCCAAAGGTT ATAAGGAAGT GAAGAAAGAC CTGATCATTA 4381 AACTGCCCAA GTACAGTCTG TTTGAGCTGG AAAACGGCAG GAAACGCATG CTGGCAAGCG 4441 CTGGAGAGCT GCAGAAAGGC AATGAACTGG CCCTGCCTTC TAAGTACGTG AACTTCCTGT 4501 ATCTGGCAAG CCACTACGAG AAGCTGAAAG GATCTCCAGA GGATAACGAA CAGAAACAGC 4561 TGTTTGTGGA GCAGCACAAG CATTATCTGG ACGAGATCAT TGAACAGATT AGCGAGTTCT 4621 CTAAAAGAGT GATCCTGGCC GACGCAAATC TGGATAAGGT CCTGTCTGCT TACAACAAAC 4681 ACAGAGATAA GCCCATCAGG GAGCAGGCCG AAAATATCAT TCATCTGTTC ACTCTGACAA 4741 ACCTGGGCGC ACCTGCAGCT TTCAAGTACT TCGACACTAC AATCGATAGA AAGAGGTACA 4801 CCTCCACTAA GGAGGTGCTG GACGCTACAC TGATCCATCA GAGTATTACC GGCCTGTACG 4861 AAACAAGGAT TGACCTGTCT CAGCTGGGTG GCGACTCACC GGTGAGATCT CCTAAGAAGA 4921 AGAGAAAGGT GTGACCGCGG AATCTAGAGC GGCCGCCACC GCGGTGGAGC TCCAGCTTTT 4981 GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG CACCCAGCTT 5041 TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG 5101 TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT 5161 CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT 5221 GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA 5281 ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA 5341 ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT 5401 CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC 5461 ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA 5521 CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT 5581 TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT 5641 CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG 5701 TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA 5761 TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG 5821 AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC 5881 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG 5941 ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC 6001 TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC 6061 CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG 6121 TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT 6181 GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA 6241 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA 6301 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC 6361 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT 6421 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC 6481 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC 6541 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA 6601 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG 6661 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 6721 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT 6781 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT 6841 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC 6901 ACATGTT //