LOCUS pME-Cas9-T2A-GFP.txt.xdna 7685 bp DNA linear 2/16/2015 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers misc_feature 744..749 /label=SalI site /SerialCloner_Color=&hFF8080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4905..4910 /label=BamHI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 756..4904 /label=Cas9 /SerialCloner_Color=&hFF00FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 569..668 /label=attL1 /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4911..4931 /label=nls2 /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 768..788 /label=nls1 /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4947..5000 /label=T2A /SerialCloner_Color=&h0000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5001..5006 /label=XbaI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5007..5726 /label=GFP /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5727..5734 /label=NotI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5810..5909 /label=attL2 /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 6079..6888 /label=KanR /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT 721 TGGGTACCGG GCCCCCCCTC GAGGTCGACG CCACCATGGC TTCTCCACCT AAGAAGAAGA 781 GAAAGGTGGG AAGCATGGAT AAGAAGTATA GCATCGGCCT GGATATTGGA ACTAACTCCG 841 TGGGTTGGGC AGTGATTACA GACGACTACA AGGTCCCTAG CAAGAAATTT AAGGTGCTGG 901 GTAACACCGA CAGGCACAGC ATCAAGAAAA ATCTGATTGG AGCCCTGCTG TTCGGTTCTG 961 GAGAGACTGC CGAAGCAACA CGCCTGAAAA GAACAGCAAG AAGGCGCTAT ACCAGAAGGA 1021 AGAATAGAAT CTGTTACCTG CAGGAGATTT TCTCTAACGA AATGGCTAAG GTGGACGATT 1081 CATTCTTTCA TAGGCTGGAG GAAAGTTTCC TGGTCGAGGA AGATAAGAAA CACGAGCGCC 1141 ATCCTATCTT TGGAAACATT GTGGACGAGG TCGCCTATCA CGAAAAATAC CCAACCATCT 1201 ATCATCTGCG CAAGAAACTG GCTGACTCTA CTGATAAAGC CGACCTGAGA CTGATCTATC 1261 TGGCTCTGGC CCACATGATT AAGTTCAGGG GTCATTTTCT GATCGAGGGC GATCTGAACC 1321 CCGACAATTC CGATGTGGAC AAGCTGTTCA TCCAGCTGGT CCAGATTTAC AATCAGCTGT 1381 TTGAGGAAAA CCCTATTAAT GCTTCCAGAG TGGACGCAAA AGCTATCCTG TCAGCCAGGC 1441 TGTCCAAGTC ACGCAGACTG GAGAACCTGA TTGCACAGCT GCCCGGAGAA AAGAGGAACG 1501 GTCTGTTTGG AAATCTGATC GCTCTGAGTC TGGGCCTGAC TCCTAACTTC AAAAGCAATT 1561 TTGATCTGGC TGAGGACGCC AAACTGCAGC TGTCAAAGGA CACATATGAC GATGACCTGG 1621 ATAACCTGCT GGCACAGATC GGAGATCAGT ACGCTGACCT GTTCCTGGCT GCCAAAAATC 1681 TGTCCGACGC AATCCTGCTG TCAGATATTC TGAGAGTGAA CAGCGAGATT ACAAAAGCAC 1741 CTCTGAGTGC CAGCATGATC AAGAGATATG ACGAGCACCA TCAGGATCTG ACCCTGCTGA 1801 AGGCTCTGGT CAGGCAGCAG CTGCCAGAGA AGTACAAGGA AATTTTCTTT GATCAGTCCA 1861 AGAACGGCTA CGCCGGTTAT ATCGACGGAG GCGCATCACA GGAGGAATTC TACAAGTTTA 1921 TCAAACCTAT TCTGGAGAAG ATGGACGGAA CTGAGGAACT GCTGGTGAAA CTGAATAGAG 1981 AGGACCTGCT GAGGAAGCAG CGCACATTTG ATAACGGTTC CATCCCACAC CAGATTCATC 2041 TGGGAGAGCT GCACGCTATC CTGAGGCGCC AGGAAGACTT CTACCCCTTT CTGAAAGATA 2101 ACCGCGAGAA GATCGAAAAA ATTCTGACCT TCAGAATCCC TTACTATGTG GGTCCACTGG 2161 CTCGCGGAAA CAGCAGATTT GCCTGGATGA CTCGCAAATC CGAGGAAACC ATTACTCCTT 2221 GGAACTTCGA GGAAGTGGTC GATAAGGGCG CCTCTGCACA GTCCTTCATC GAGAGAATGA 2281 CTAATTTTGA CAAAAACCTG CCCAATGAGA AAGTGCTGCC TAAGCACTCC CTGCTGTACG 2341 AGTATTTCAC TGTCTATAAC GAACTGACAA AGGTGAAATA CGTCACCGAG GGCATGAGAA 2401 AGCCAGCCTT CCTGTCAGGA GAGCAGAAGA AAGCAATCGT GGATCTGCTG TTTAAAACCA 2461 ACAGGAAAGT GACTGTCAAG CAGCTGAAGG AGGACTACTT CAAGAAAATT GAATGCTTCG 2521 ATTCCGTGGA GATCAGCGGA GTCGAAGACA GATTTAACGC AAGCCTGGGC GCTTACCACG 2581 ATCTGCTGAA GATCATTAAG GATAAAGACT TCCTGGACAA CGAGGAAAAT GAGGATATCC 2641 TGGAAGACAT TGTGCTGACA CTGACCCTGT TTGAGGACAG AGGAATGATC GAGGAAAGAC 2701 TGAAAACCTA TGCTCATCTG TTCGATGACA AGGTGATGAA ACAGCTGAAG AGAAGGCGCT 2761 ACACTGGCTG GGGTAGACTG AGCAGGAAGC TGATCAACGG CATTAGGGAT AAACAGTCAG 2821 GAAAGACAAT CCTGGACTTT CTGAAAAGTG ATGGCTTCGC CAACCGCAAT TTTATGCAGC 2881 TGATTCACGA TGACAGTCTG ACCTTCAAAG AGGACATCCA GAAGGCTCAG GTGTCTGGAC 2941 AGGGCCACTC CCTGCATGAG CAGATTGCAA ACCTGGCTGG AAGCCCAGCC ATCAAGAAAG 3001 GCATTCTGCA GACAGTGAAA ATCGTCGATG AGCTGGTGAA AGTCATGGGC CATAAGCCCG 3061 AAAACATCGT GATTGAGATG GCTCGCGAAA ATCAGACAAC CCAGAAGGGT CAGAAGAACA 3121 GTAGAGAGAG GATGAAAAGA ATCGAGGAAG GCATTAAGGA GCTGGGTAGC CAGATCCTGA 3181 AAGAGCACCC AGTGGAAAAC ACACAGCTGC AGAATGAGAA GCTGTATCTG TACTATCTGC 3241 AGAATGGAAG AGATATGTAC GTGGACCAGG AGCTGGATAT TAACAGGCTG TCTGATTACG 3301 ACGTGGATCA TATCGTCCCC CAGAGTTTCA TCAAAGATGA CAGCATTGAC AACAAGGTGC 3361 TGACCAGGTC CGACAAAAAC AGAGGAAAAT CAGATAATGT CCCTAGTGAG GAAGTGGTCA 3421 AGAAAATGAA GAACTACTGG AGACAGCTGC TGAATGCCAA ACTGATCACT CAGAGGAAGT 3481 TTGATAACCT GACAAAAGCA GAGCGCGGTG GACTGTCAGA ACTGGACAAA GCTGGATTCA 3541 TCAAGAGGCA GCTGGTGGAA ACACGCCAGA TCACTAAACA CGTCGCACAG ATTCTGGATA 3601 GTCGCATGAA CACAAAGTAC GATGAGAATG ACAAACTGAT CAGAGAAGTG AAGGTCATTA 3661 CCCTGAAGAG TAAACTGGTC AGCGACTTTA GGAAAGATTT CCAGTTTTAT AAGGTCCGCG 3721 AGATTAACAA TTATCACCAT GCCCATGACG CATACCTGAA CGCCGTGGTC GGTACCGCAC 3781 TGATCAAGAA ATACCCAAAA CTGGAGAGCG AATTCGTGTA CGGAGACTAT AAGGTGTACG 3841 ATGTCAGAAA AATGATCGCC AAGTCCGAGC AGGAAATTGG AAAAGCTACT GCCAAGTATT 3901 TCTTTTACTC AAACATCATG AATTTCTTTA AGACAGAGAT CACCCTGGCC AATGGAGAAA 3961 TCCGCAAAAG GCCCCTGATT GAGACAAACG GAGAGACAGG CGAAATCGTG TGGGACAAAG 4021 GCAGAGATTT TGCAACCGTG AGGAAGGTCC TGAGCATGCC TCAAGTGAAT ATCGTCAAGA 4081 AAACTGAGGT GCAGACAGGC GGTTTCTCAA AAGAAAGTAT TCTGCCAAAA CGCAACTCTG 4141 ATAAGCTGAT CGCTAGAAAG AAAGACTGGG ACCCTAAGAA GTATGGAGGC TTTGACTCTC 4201 CCACTGTGGC ATACTCCGTC CTGGTGGTCG CTAAGGTGGA GAAGGGCAAA AGCAAGAAAC 4261 TGAAATCTGT CAAGGAGCTG CTGGGTATCA CAATTATGGA GAGAAGCTCT TTCGAGAAGA 4321 ACCCAATCGA TTTTCTGGAG GCCAAAGGTT ATAAGGAAGT GAAGAAAGAC CTGATCATTA 4381 AACTGCCCAA GTACAGTCTG TTTGAGCTGG AAAACGGCAG GAAACGCATG CTGGCAAGCG 4441 CTGGAGAGCT GCAGAAAGGC AATGAACTGG CCCTGCCTTC TAAGTACGTG AACTTCCTGT 4501 ATCTGGCAAG CCACTACGAG AAGCTGAAAG GATCTCCAGA GGATAACGAA CAGAAACAGC 4561 TGTTTGTGGA GCAGCACAAG CATTATCTGG ACGAGATCAT TGAACAGATT AGCGAGTTCT 4621 CTAAAAGAGT GATCCTGGCC GACGCAAATC TGGATAAGGT CCTGTCTGCT TACAACAAAC 4681 ACAGAGATAA GCCCATCAGG GAGCAGGCCG AAAATATCAT TCATCTGTTC ACTCTGACAA 4741 ACCTGGGCGC ACCTGCAGCT TTCAAGTACT TCGACACTAC AATCGATAGA AAGAGGTACA 4801 CCTCCACTAA GGAGGTGCTG GACGCTACAC TGATCCATCA GAGTATTACC GGCCTGTACG 4861 AAACAAGGAT TGACCTGTCT CAGCTGGGTG GCGACTCACC GGTGGGATCC CCTAAGAAGA 4921 AGAGAAAGGT GAGGTCTGGT GGCGGAGAGG GCAGAGGCAG TCTGCTGACA TGCGGTGACG 4981 TGGAAGAGAA TCCCGGCCCT TCTAGAATGG TGAGCAAGGG CGAAGAGCTG TTCACCGGGG 5041 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG 5101 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 5161 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT 5221 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG 5281 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG 5341 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA 5401 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT 5461 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA 5521 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG 5581 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC 5641 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC 5701 TCGGCATGGA CGAGCTGTAC AAGTAAGCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT 5761 TCCCTTTAGT GAGGGTTAAT TGCGCGCTTG GCGTAATCAT GGTCATAGCA CCCAGCTTTC 5821 TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC 5881 ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG 5941 TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT 6001 TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC 6061 AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT 6121 TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA 6181 GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT 6241 GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG 6301 GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA 6361 CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA 6421 GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT 6481 TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG 6541 AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA 6601 CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT 6661 GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT 6721 GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC 6781 GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT 6841 GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT 6901 AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA 6961 CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 7021 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 7081 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 7141 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG 7201 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 7261 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 7321 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 7381 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC 7441 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 7501 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 7561 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 7621 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 7681 ATGTT //