LOCUS pDestTol2CG2-U6_gRNA.txt.xdna 8267 bp DNA circular 2/16/2015 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS complement(125..982) /label=ampR /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True rep_origin complement(1130..1803) /label=pUC ori /SerialCloner_Color=&h0080FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True LTR complement(2272..2290) /label=Tol2 5' TIR /SerialCloner_Color=&h0080FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True promoter complement(2795..3693) /label=cmlc2 promoter /SerialCloner_Color=&hFF8080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True CDS complement(3694..4410) /label=EGFP [*] /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature complement(3885..3898) /label=EGFP-like chromophore /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature complement(3709..3714) /label=mutated BseRI restriction site /SerialCloner_Color=&hFF0080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True polyA_signal complement(4652..4849) /label=SV40 late polyA (from pCS2+) /SerialCloner_Color=&hFF80FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4853..4858 /label=ClaI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5330..5335 /label=KpnI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4859..5214 /label=U6 promoter /SerialCloner_Color=&hFF0000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5248..5329 /label=gRNA scaffold /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5360..5484 /label=attR3 /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5593..6252 /label=cmR /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 6594..6899 /label=ccdB /SerialCloner_Color=&h00FFFF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_recomb complement(6939..7063) /label=attR4 /SerialCloner_Color=&h8080FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5222..5227 /label=BseRI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5234..5239 /label=BseRI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 61 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 121 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 181 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 241 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 301 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 361 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 421 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 481 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 541 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 601 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 661 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 721 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 781 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 841 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 901 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 961 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 1021 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 1081 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 1141 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 1201 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 1261 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 1321 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 1381 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 1441 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 1501 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 1561 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 1621 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 1681 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 1741 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 1801 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 1861 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 1921 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 1981 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 2041 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 2101 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA 2161 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG 2221 CTCTAGAACT AGTGGATCTG CTGGGCTTGC TGAAGGTAGG GGGTCAAGAA CCAGAGGTGT 2281 AAAGTACTTG AGTAATTTTA CTTGATTACT GTACTTAAGT ATTATTTTTG GGGATTTTTA 2341 CTTTACTTGA GTACAATTAA AAATCAATAC TTTTACTTTT ACTTAATTAC ATTTTTTTAG 2401 AAAAAAAAGT ACTTTTTACT CCTTACAATT TTATTTACAG TCAAAAAGTA CTTATTTTTT 2461 GGAGATCACT TCATTCTATT TTCCCTTGCT ATTACCAAAC CAATTGAATT GCGCTGATGC 2521 CCAGTTTAAT TTAAATGTTA TTTATTCTGC CTATGAAAAT CGTTTTCACA TTATATGAAA 2581 TTGGTCAGAC ATGTTCATTG GTCCTTTGGA AGTGACGTCA TGTCACATCT ATTACCACAA 2641 TGCACAGCAC CTTGACCTGG AAATTAGGGA AATTATAACA GTCAATCAGT GGAAGAAAAT 2701 GGAGGAAGTA TGTGATTCAT CAGCAGCTGC GAGCAGCACA GTCCAAAATC AGCCACAGGA 2761 TCAAGAGCAC CCGTGGCCGT ATCTTCGCAG ATCTAAAGCT TAAATCAGTT GTGTTAAATA 2821 AGAGACATTC AAAATAAATG TAAATGAGCT CTCCAAATCA GCAGACTTAA CATTCTTTAA 2881 AATGATTGAT TCAATAGTGA TAAAAATCAG GCATAGCCAG TTGTAACTTT AGATAAATTA 2941 CAGAAAATGT CAAATACAGA GAACCGATTC TTTTTTATGA TACATCCAAG CACACATTTA 3001 ACACAATCCA GGCAAACCCC GAATTTCACA GTCACAAGCA CTGTTTGTAC AAGAGCTTTG 3061 CCTAAGGACA CACAGTCTCT ATAAGTCCAG GTCGTTGGTT TCACTCTTAT TTTAAACATG 3121 TGACATTTTT CCTGCCATCC TGTCTTAGGC TGCTGTTTGC TTCATTCCAT GTCACATTAA 3181 ATTCCTCAGT AGCACCTTTT ACACACACAG CCAATCTTTT CCAGAAAATT CAATTGCTTT 3241 GAAGAGATAA TGTGTGAACA AATCCATTTA GAAAAGGAAA ATTAAGAATT TGTAAAATCA 3301 TCTGTAAATT GTTGGCATTC TTCTGTATAT GAACATCACA TCATTTACAG GTAAAGGTCT 3361 GGTCATTAAT TATATGACAA TTTACTGGTA TTATTTTGTG AAAGGGGCTA TTTTCAATGC 3421 GTTCATCCAT CCTTTTCATC CCTCAAATCT CTCATTCACG TCCCCCTCCC CATCTGCACA 3481 CTTTATCTCA TTTTCCACCC TGCTGGAATC TGAGCACTTG TGCAGTTATC AGGGCTCCTG 3541 TATTTAGGAG GCTCTGGGTG TCCATGTAGG GGACGAACAG AAACACTGCA GACCTTTATA 3601 GAAGAACAAT TGATAAGAGT CCTCATACAT AAAGACTCCA TTAGTAAGCC AGTGACCCAG 3661 GAGCCCAGAC CAACAGCAAA GCAGACAGTG ACCATGGTGA GCAAGGGCGA AGAGCTGTTC 3721 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC 3781 GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC 3841 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG 3901 CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG 3961 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC 4021 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC 4081 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC 4141 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC 4201 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC 4261 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC 4321 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG 4381 ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC CCTCTCGAGC CTCTAGAACT 4441 ATAGTGAGTC GTATTACGTA GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA 4501 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT 4561 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGAGATCTG ATCTAGAGGA TCATAATCAG 4621 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA 4681 CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG 4741 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 4801 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCA TCATCGATGC 4861 GTCTTTTGTT CTGGTCATCA AGGAGGGGGG AATGTTCTGC GCATGCCTGT GGGGGAGGGA 4921 GAAGGACACG TCACTGAAAA CGTCCCTGCA TCACACCGAG ACACCCAATC ACTCAAGCCG 4981 AGACCAGATA ATTTTGCATA TGCTTTACAG TTTGAAAAAT ACCACGGTAA ACCCTCACAC 5041 AAACTCTGGA TTTGAGATCT TTCAGGTTTT ATCAGTTTGC AGGTTTATGT CACCATGATA 5101 TAGGGTCAGA CTTGATCTAA GGGAGCTGAA TAAGTGGTTT AGTCACTCAC CACCTCCCAA 5161 AAACATACCC AGAAGTCCCT GGTATATATA GCTCTCCCTC CAGCTCTTGG TTCGGCTAGC 5221 ACTCCTCAGT GACGAGGAGT CAGTAGCGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG 5281 GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTG GTACCGTAAA 5341 ACGACGGCCA GTGAATTATC AACTATGTAT AATAAAGTTG AACGAGAAAC GTAAAATGAT 5401 ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA 5461 ACACAACATA TCCAGTCACT ATGGCGGCCG CATTAGGCAC CCCCAGGCTT TACACTTTAT 5521 GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT 5581 AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG 5641 CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC 5701 GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT 5761 CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA 5821 ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT 5881 GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT 5941 CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA 6001 GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT 6061 GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT 6121 TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTTTGT 6181 GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG 6241 GGCGGGGCGT AATCTAGAGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG 6301 CGCGCTGATT TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA 6361 AGAGGTATGC TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC 6421 TCAAGGCATA TATGATGTCA ATATCTCCGG TTCGGTAAGC ACAACCATGC AGAATGAAGC 6481 CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC 6541 CCGGTTTATT GAAATGAACG GCTCTTTTGC CGACGAGAAC AGGGGCTGGT GAAATGCAGT 6601 TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG 6661 ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT 6721 CAGATAAAGT CCCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA 6781 TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC 6841 TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA 6901 TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT 6961 GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT 7021 ATATCATTTT ACGTTTCTCG TTCAACTTTT CTATACAAAG TTGATAGCTT GGCGTAATCA 7081 TGGTCATAGC TGTTTCCTGC TCGAGGGCCC ATCTGGCCTG TGTTTCAGAC ACCAGGGAGT 7141 CTCTGCTCAC GTTTCCTGCT ATTTGCAGCC TCTCTATCAA GACTAATACA CCTCTTCCCG 7201 CATCGGCTGC CTGTGAGAGG CTTTTCAGCA CTGCAGGATT GCTTTTCAGC CCCAAAAGAG 7261 CTAGGCTTGA CACTAACAAT TTTGAGAATC AGCTTCTACT GAAGTTAAAT CTGAGGTTTT 7321 ACAACTTTGA GTAGCGTGTA CTGGCATTAG ATTGTCTGTC TTATAGTTTG ATAATTAAAT 7381 ACAAACAGTT CTAAAGCAGG ATAAAACCTT GTATGCATTT CATTTAATGT TTTTTGAGAT 7441 TAAAAGCTTA AACAAGAATC TCTAGTTTTC TTTCTTGCTT TTACTTTTAC TTCCTTAATA 7501 CTCAAGTACA ATTTTAATGG AGTACTTTTT TACTTTTACT CAAGTAAGAT TCTAGCCAGA 7561 TACTTTTACT TTTAATTGAG TAAAATTTTC CCTAAGTACT TGTACTTTCA CTTGAGTAAA 7621 ATTTTTGAGT ACTTTTTACA CCTCTGTCAA GAACTCCTGG ACAAACCTCT GACCTGTGTG 7681 GAACAGAGTG GATATGGGTG TCTGAACAGA TATTCACGTC TTTTGCAGAT CAGAGGGCAT 7741 TTCTGGTGTT GTGCAATGAC CTGGGTCCAA CAGGTAGGCG TACTCGTATC CAAGAGCCTT 7801 GTTGGACAGG AGGTAGTCTC CATTCCTATA AAGAGGAAGG AATGGCACCA TGTAGTAGCC 7861 GTCATTGTGG CCAATTGGTG CATTGGTACG TGGGTAGATG GACCGGGGAG GCTGATGAGT 7921 TCTTAGCCAG CCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG 7981 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG 8041 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 8101 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 8161 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 8221 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAGGTGG //