LOCUS       pDestTol2CG2-U6_gRNA.txt.xdna    8267 bp      DNA     circular   2/16/2015
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             complement(125..982)
                     /label=ampR
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(1130..1803)
                     /label=pUC ori
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     LTR             complement(2272..2290)
                     /label=Tol2 5' TIR
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     promoter        complement(2795..3693)
                     /label=cmlc2 promoter
                     /SerialCloner_Color=&hFF8080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(3694..4410)
                     /label=EGFP [*]
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(3885..3898)
                     /label=EGFP-like chromophore
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(3709..3714)
                     /label=mutated BseRI restriction site
                     /SerialCloner_Color=&hFF0080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     polyA_signal    complement(4652..4849)
                     /label=SV40 late polyA (from pCS2+)
                     /SerialCloner_Color=&hFF80FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4853..4858
                     /label=ClaI site
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5330..5335
                     /label=KpnI site
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4859..5214
                     /label=U6 promoter
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5248..5329
                     /label=gRNA scaffold
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5360..5484
                     /label=attR3
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5593..6252
                     /label=cmR
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6594..6899
                     /label=ccdB
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_recomb     complement(6939..7063)
                     /label=attR4
                     /SerialCloner_Color=&h8080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5222..5227
                     /label=BseRI site
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5234..5239
                     /label=BseRI site
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 
       61 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 
      121 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 
      181 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 
      241 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 
      301 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 
      361 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 
      421 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 
      481 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 
      541 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 
      601 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 
      661 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 
      721 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 
      781 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 
      841 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 
      901 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 
      961 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 
     1021 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 
     1081 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 
     1141 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 
     1201 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 
     1261 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 
     1321 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 
     1381 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 
     1441 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 
     1501 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 
     1561 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 
     1621 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 
     1681 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 
     1741 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 
     1801 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 
     1861 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 
     1921 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 
     1981 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 
     2041 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 
     2101 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA 
     2161 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG 
     2221 CTCTAGAACT AGTGGATCTG CTGGGCTTGC TGAAGGTAGG GGGTCAAGAA CCAGAGGTGT 
     2281 AAAGTACTTG AGTAATTTTA CTTGATTACT GTACTTAAGT ATTATTTTTG GGGATTTTTA 
     2341 CTTTACTTGA GTACAATTAA AAATCAATAC TTTTACTTTT ACTTAATTAC ATTTTTTTAG 
     2401 AAAAAAAAGT ACTTTTTACT CCTTACAATT TTATTTACAG TCAAAAAGTA CTTATTTTTT 
     2461 GGAGATCACT TCATTCTATT TTCCCTTGCT ATTACCAAAC CAATTGAATT GCGCTGATGC 
     2521 CCAGTTTAAT TTAAATGTTA TTTATTCTGC CTATGAAAAT CGTTTTCACA TTATATGAAA 
     2581 TTGGTCAGAC ATGTTCATTG GTCCTTTGGA AGTGACGTCA TGTCACATCT ATTACCACAA 
     2641 TGCACAGCAC CTTGACCTGG AAATTAGGGA AATTATAACA GTCAATCAGT GGAAGAAAAT 
     2701 GGAGGAAGTA TGTGATTCAT CAGCAGCTGC GAGCAGCACA GTCCAAAATC AGCCACAGGA 
     2761 TCAAGAGCAC CCGTGGCCGT ATCTTCGCAG ATCTAAAGCT TAAATCAGTT GTGTTAAATA 
     2821 AGAGACATTC AAAATAAATG TAAATGAGCT CTCCAAATCA GCAGACTTAA CATTCTTTAA 
     2881 AATGATTGAT TCAATAGTGA TAAAAATCAG GCATAGCCAG TTGTAACTTT AGATAAATTA 
     2941 CAGAAAATGT CAAATACAGA GAACCGATTC TTTTTTATGA TACATCCAAG CACACATTTA 
     3001 ACACAATCCA GGCAAACCCC GAATTTCACA GTCACAAGCA CTGTTTGTAC AAGAGCTTTG 
     3061 CCTAAGGACA CACAGTCTCT ATAAGTCCAG GTCGTTGGTT TCACTCTTAT TTTAAACATG 
     3121 TGACATTTTT CCTGCCATCC TGTCTTAGGC TGCTGTTTGC TTCATTCCAT GTCACATTAA 
     3181 ATTCCTCAGT AGCACCTTTT ACACACACAG CCAATCTTTT CCAGAAAATT CAATTGCTTT 
     3241 GAAGAGATAA TGTGTGAACA AATCCATTTA GAAAAGGAAA ATTAAGAATT TGTAAAATCA 
     3301 TCTGTAAATT GTTGGCATTC TTCTGTATAT GAACATCACA TCATTTACAG GTAAAGGTCT 
     3361 GGTCATTAAT TATATGACAA TTTACTGGTA TTATTTTGTG AAAGGGGCTA TTTTCAATGC 
     3421 GTTCATCCAT CCTTTTCATC CCTCAAATCT CTCATTCACG TCCCCCTCCC CATCTGCACA 
     3481 CTTTATCTCA TTTTCCACCC TGCTGGAATC TGAGCACTTG TGCAGTTATC AGGGCTCCTG 
     3541 TATTTAGGAG GCTCTGGGTG TCCATGTAGG GGACGAACAG AAACACTGCA GACCTTTATA 
     3601 GAAGAACAAT TGATAAGAGT CCTCATACAT AAAGACTCCA TTAGTAAGCC AGTGACCCAG 
     3661 GAGCCCAGAC CAACAGCAAA GCAGACAGTG ACCATGGTGA GCAAGGGCGA AGAGCTGTTC 
     3721 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC 
     3781 GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC 
     3841 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG 
     3901 CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG 
     3961 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC 
     4021 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC 
     4081 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC 
     4141 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC 
     4201 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC 
     4261 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC 
     4321 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG 
     4381 ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC CCTCTCGAGC CTCTAGAACT 
     4441 ATAGTGAGTC GTATTACGTA GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA 
     4501 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT 
     4561 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGAGATCTG ATCTAGAGGA TCATAATCAG 
     4621 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA 
     4681 CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG 
     4741 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 
     4801 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCA TCATCGATGC 
     4861 GTCTTTTGTT CTGGTCATCA AGGAGGGGGG AATGTTCTGC GCATGCCTGT GGGGGAGGGA 
     4921 GAAGGACACG TCACTGAAAA CGTCCCTGCA TCACACCGAG ACACCCAATC ACTCAAGCCG 
     4981 AGACCAGATA ATTTTGCATA TGCTTTACAG TTTGAAAAAT ACCACGGTAA ACCCTCACAC 
     5041 AAACTCTGGA TTTGAGATCT TTCAGGTTTT ATCAGTTTGC AGGTTTATGT CACCATGATA 
     5101 TAGGGTCAGA CTTGATCTAA GGGAGCTGAA TAAGTGGTTT AGTCACTCAC CACCTCCCAA 
     5161 AAACATACCC AGAAGTCCCT GGTATATATA GCTCTCCCTC CAGCTCTTGG TTCGGCTAGC 
     5221 ACTCCTCAGT GACGAGGAGT CAGTAGCGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG 
     5281 GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTG GTACCGTAAA 
     5341 ACGACGGCCA GTGAATTATC AACTATGTAT AATAAAGTTG AACGAGAAAC GTAAAATGAT 
     5401 ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA 
     5461 ACACAACATA TCCAGTCACT ATGGCGGCCG CATTAGGCAC CCCCAGGCTT TACACTTTAT 
     5521 GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT 
     5581 AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG 
     5641 CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC 
     5701 GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT 
     5761 CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA 
     5821 ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT 
     5881 GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT 
     5941 CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA 
     6001 GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT 
     6061 GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT 
     6121 TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTTTGT 
     6181 GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG 
     6241 GGCGGGGCGT AATCTAGAGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG 
     6301 CGCGCTGATT TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA 
     6361 AGAGGTATGC TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC 
     6421 TCAAGGCATA TATGATGTCA ATATCTCCGG TTCGGTAAGC ACAACCATGC AGAATGAAGC 
     6481 CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC 
     6541 CCGGTTTATT GAAATGAACG GCTCTTTTGC CGACGAGAAC AGGGGCTGGT GAAATGCAGT 
     6601 TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG 
     6661 ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT 
     6721 CAGATAAAGT CCCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA 
     6781 TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC 
     6841 TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA 
     6901 TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT 
     6961 GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT 
     7021 ATATCATTTT ACGTTTCTCG TTCAACTTTT CTATACAAAG TTGATAGCTT GGCGTAATCA 
     7081 TGGTCATAGC TGTTTCCTGC TCGAGGGCCC ATCTGGCCTG TGTTTCAGAC ACCAGGGAGT 
     7141 CTCTGCTCAC GTTTCCTGCT ATTTGCAGCC TCTCTATCAA GACTAATACA CCTCTTCCCG 
     7201 CATCGGCTGC CTGTGAGAGG CTTTTCAGCA CTGCAGGATT GCTTTTCAGC CCCAAAAGAG 
     7261 CTAGGCTTGA CACTAACAAT TTTGAGAATC AGCTTCTACT GAAGTTAAAT CTGAGGTTTT 
     7321 ACAACTTTGA GTAGCGTGTA CTGGCATTAG ATTGTCTGTC TTATAGTTTG ATAATTAAAT 
     7381 ACAAACAGTT CTAAAGCAGG ATAAAACCTT GTATGCATTT CATTTAATGT TTTTTGAGAT 
     7441 TAAAAGCTTA AACAAGAATC TCTAGTTTTC TTTCTTGCTT TTACTTTTAC TTCCTTAATA 
     7501 CTCAAGTACA ATTTTAATGG AGTACTTTTT TACTTTTACT CAAGTAAGAT TCTAGCCAGA 
     7561 TACTTTTACT TTTAATTGAG TAAAATTTTC CCTAAGTACT TGTACTTTCA CTTGAGTAAA 
     7621 ATTTTTGAGT ACTTTTTACA CCTCTGTCAA GAACTCCTGG ACAAACCTCT GACCTGTGTG 
     7681 GAACAGAGTG GATATGGGTG TCTGAACAGA TATTCACGTC TTTTGCAGAT CAGAGGGCAT 
     7741 TTCTGGTGTT GTGCAATGAC CTGGGTCCAA CAGGTAGGCG TACTCGTATC CAAGAGCCTT 
     7801 GTTGGACAGG AGGTAGTCTC CATTCCTATA AAGAGGAAGG AATGGCACCA TGTAGTAGCC 
     7861 GTCATTGTGG CCAATTGGTG CATTGGTACG TGGGTAGATG GACCGGGGAG GCTGATGAGT 
     7921 TCTTAGCCAG CCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG 
     7981 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG 
     8041 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 
     8101 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 
     8161 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 
     8221 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAGGTGG
//