LOCUS pDestTol2pA2-U6_gRNA.txt.xdna 6354 bp DNA circular 2/16/2015 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers misc_feature 125..982 /label=ampR /SerialCloner_Color=&h8080FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 1130..1803 /label=pUC ori /SerialCloner_Color=&h80FF80 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 2272..2290 /label=Tol2 5' TIR /SerialCloner_Color=&h0080FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 2847..3044 /label=SV40 late PolyA /SerialCloner_Color=&hFF80C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3048..3053 /label=ClaI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3054..3409 /label=U6 promoter /SerialCloner_Color=&hFF0000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3443..3524 /label=gRNA scaffold /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3525..3530 /label=KpnI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3555..3679 /label=attR3 /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3788..4447 /label=cmR /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 4789..5094 /label=ccdB /SerialCloner_Color=&h80FFFF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 5134..5258 /label=attR4 /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3417..3422 /label=BseRI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3429..3434 /label=BseRI site /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 61 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 121 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 181 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 241 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 301 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 361 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 421 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 481 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 541 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 601 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 661 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 721 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 781 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 841 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 901 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 961 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 1021 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 1081 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 1141 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 1201 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 1261 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 1321 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 1381 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 1441 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 1501 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 1561 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 1621 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 1681 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 1741 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 1801 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 1861 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 1921 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 1981 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 2041 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 2101 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA 2161 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG 2221 CTCTAGAACT AGTGGATCTG CTGGGCTTGC TGAAGGTAGG GGGTCAAGAA CCAGAGGTGT 2281 AAAGTACTTG AGTAATTTTA CTTGATTACT GTACTTAAGT ATTATTTTTG GGGATTTTTA 2341 CTTTACTTGA GTACAATTAA AAATCAATAC TTTTACTTTT ACTTAATTAC ATTTTTTTAG 2401 AAAAAAAAGT ACTTTTTACT CCTTACAATT TTATTTACAG TCAAAAAGTA CTTATTTTTT 2461 GGAGATCACT TCATTCTATT TTCCCTTGCT ATTACCAAAC CAATTGAATT GCGCTGATGC 2521 CCAGTTTAAT TTAAATGTTA TTTATTCTGC CTATGAAAAT CGTTTTCACA TTATATGAAA 2581 TTGGTCAGAC ATGTTCATTG GTCCTTTGGA AGTGACGTCA TGTCACATCT ATTACCACAA 2641 TGCACAGCAC CTTGACCTGG AAATTAGGGA AATTATAACA GTCAATCAGT GGAAGAAAAT 2701 GGAGGAAGTA TGTGATTCAT CAGCAGCTGC GAGCAGCACA GTCCAAAATC AGCCACAGGA 2761 TCAAGAGCAC CCGTGGCCGT ATCTTCGCAG ATCTGATCTA GAGGATCATA ATCAGCCATA 2821 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA 2881 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 2941 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 3001 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCATCATC GATGCGTCTT 3061 TTGTTCTGGT CATCAAGGAG GGGGGAATGT TCTGCGCATG CCTGTGGGGG AGGGAGAAGG 3121 ACACGTCACT GAAAACGTCC CTGCATCACA CCGAGACACC CAATCACTCA AGCCGAGACC 3181 AGATAATTTT GCATATGCTT TAAAGTTTGA AAAATACCAC GGTAAACCCT CACACAAACT 3241 CTGGATTTGA GATCTTTCAG GTTTTATCAG TTTGCAGGTT TATGTCACCA TGATATAGGG 3301 TCAGACTTGA TCTAAGGGAG CTGAATAAGT GGTTTAGTCA CTCACCACCT CCCAAAAACA 3361 TACCCAGAAG TCCCTGGTAT ATATAGCTCT CCCTCCAGCT CTTGGTTCGG CTAGCACTCC 3421 TCAGTGACGA GGAGTCAGTA GCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG 3481 TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTGGTACC GTAAAACGAC 3541 GGCCAGTGAA TTATCAACTA TGTATAATAA AGTTGAACGA GAAACGTAAA ATGATATAAA 3601 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA 3661 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCCA GGCTTTACAC TTTATGCTTC 3721 CGGCTCGTAT AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA 3781 AGCTAAAATG GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG 3841 TAAAGAACAT TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA 3901 GCTGGATATT ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC 3961 CTTTATTCAC ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA 4021 AGACGGTGAG CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA 4081 AACTGAAACG TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA 4141 CATATATTCG CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT 4201 TATTGAGAAT ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT 4261 AAACGTGGCC AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGGGCA AATATTATAC 4321 GCAAGGCGAC AAGGTGCTGA TGCCGCTGGC GATTCAGGTT CATCATGCCG TTTGTGATGG 4381 CTTCCATGTC GGCAGAATGC TTAATGAATT ACAACAGTAC TGCGATGAGT GGCAGGGCGG 4441 GGCGTAATCT AGAGGATCCG GCTTACTAAA AGCCAGATAA CAGTATGCGT ATTTGCGCGC 4501 TGATTTTTGC GGTATAAGAA TATATACTGA TATGTATACC CGAAGTATGT CAAAAAGAGG 4561 TATGCTATGA AGCAGCGTAT TACAGTGACA GTTGACAGCG ACAGCTATCA GTTGCTCAAG 4621 GCATATATGA TGTCAATATC TCCGGTTCGG TAAGCACAAC CATGCAGAAT GAAGCCCGTC 4681 GTCTGCGTGC CGAACGCTGG AAAGCGGAAA ATCAGGAAGG GATGGCTGAG GTCGCCCGGT 4741 TTATTGAAAT GAACGGCTCC TTTGCCGACG AGAACAGGGG CTGGTGAAAT GCAGTTTAAG 4801 GTTTACACCT ATAAAAGAGA GAGCCGTTAT CGTCTGTTTG TGGATGTACA GAGTGATATT 4861 ATTGACACGC CCGGGCGACG GATGGTGATC CCCCTGGCCA GTGCACGTCT GCTGTCAGAT 4921 AAAGTCCCCC GTGAACTTTA CCCGGTGGTG CATATCGGGG ATGAAAGCTG GCGCATGATG 4981 ACCACCGATA TGGCCAGTGT GCCGGTCTCC GTTATCGGGG AAGAAGTGGC TGATCTCAGC 5041 CACCGCGAAA ATGACATCAA AAACGCCATT AACCTGATGT TCTGGGGAAT ATAAATGTCA 5101 GGCTCCCTTA TACACAGCCA GTCTGCAGGT CGACCATAGT GACTGGATAT GTTGTGTTTT 5161 ACAGTATTAT GTAGTCTGTT TTTTATGCAA AATCTAATTT AATATATTGA TATTTATATC 5221 ATTTTACGTT TCTCGTTCAA CTTTTCTATA CAAAGTTGAT AGCTTGGCGT AATCATGGTC 5281 ATAGCTGTTT CCTGCTCGAG GGCCCATCTG GCCTGTGTTT CAGACACCAG GGAGTCTCTG 5341 CTCACGTTTC CTGCTATTTG CAGCCTCTCT ATCAAGACTA ATACACCTCT TCCCGCATCG 5401 GCTGCCTGTG AGAGGCTTTT CAGCACTGCA GGATTGCTTT TCAGCCCCAA AAGAGCTAGG 5461 CTTGACACTA ACAATTTTGA GAATCAGCTT CTACTGAAGT TAAATCTGAG GTTTTACAAC 5521 TTTGAGTAGC GTGTACTGGC ATTAGATTGT CTGTCTTATA GTTTGATAAT TAAATACAAA 5581 CAGTTCTAAA GCAGGATAAA ACCTTGTATG CATTTCATTT AATGTTTTTT GAGATTAAAA 5641 GCTTAAACAA GAATCTCTAG TTTTCTTTCT TGCTTTTACT TTTACTTCCT TAATACTCAA 5701 GTACAATTTT AATGGAGTAC TTTTTTACTT TTACTCAAGT AAGATTCTAG CCAGATACTT 5761 TTACTTTTAA TTGAGTAAAA TTTTCCCTAA GTACTTGTAC TTTCACTTGA GTAAAATTTT 5821 TGAGTACTTT TTACACCTCT GTCAAGAACT CCTGGACAAA CCTCTGACCT GTGTGGAACA 5881 GAGTGGATAT GGGTGTCTGA ACAGATATTC ACGTCTTTTG CAGATCAGAG GGCATTTCTG 5941 GTGTTGTGCA ATGACCTGGG TCCAACAGGT AGGCGTACTC GTATCCAAGA GCCTTGTTGG 6001 ACAGGAGGTA GTCTCCATTC CTATAAAGAG GAAGGAATGG CACCATGTAG TAGCCGTCAT 6061 TGTGGCCAAT TGGTGCATTG GTACGTGGGT AGATGGACCG GGGAGGCTGA TGAGTTCTTA 6121 GCCAGCGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 6181 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 6241 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 6301 AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG GTGG //