LOCUS       pDestTol2pA2-U6_gRNA.txt.xdna    6354 bp      DNA     circular   2/16/2015
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    125..982
                     /label=ampR
                     /SerialCloner_Color=&h8080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1130..1803
                     /label=pUC ori
                     /SerialCloner_Color=&h80FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2272..2290
                     /label=Tol2 5' TIR
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2847..3044
                     /label=SV40 late PolyA
                     /SerialCloner_Color=&hFF80C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3048..3053
                     /label=ClaI site
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3054..3409
                     /label=U6 promoter
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3443..3524
                     /label=gRNA scaffold
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3525..3530
                     /label=KpnI site
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3555..3679
                     /label=attR3
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3788..4447
                     /label=cmR
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4789..5094
                     /label=ccdB
                     /SerialCloner_Color=&h80FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5134..5258
                     /label=attR4
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3417..3422
                     /label=BseRI site
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3429..3434
                     /label=BseRI site
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 
       61 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 
      121 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 
      181 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 
      241 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 
      301 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 
      361 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 
      421 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 
      481 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 
      541 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 
      601 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 
      661 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 
      721 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 
      781 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 
      841 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 
      901 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 
      961 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 
     1021 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 
     1081 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 
     1141 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 
     1201 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 
     1261 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 
     1321 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 
     1381 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 
     1441 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 
     1501 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 
     1561 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 
     1621 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 
     1681 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 
     1741 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 
     1801 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 
     1861 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 
     1921 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 
     1981 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 
     2041 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 
     2101 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA 
     2161 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG 
     2221 CTCTAGAACT AGTGGATCTG CTGGGCTTGC TGAAGGTAGG GGGTCAAGAA CCAGAGGTGT 
     2281 AAAGTACTTG AGTAATTTTA CTTGATTACT GTACTTAAGT ATTATTTTTG GGGATTTTTA 
     2341 CTTTACTTGA GTACAATTAA AAATCAATAC TTTTACTTTT ACTTAATTAC ATTTTTTTAG 
     2401 AAAAAAAAGT ACTTTTTACT CCTTACAATT TTATTTACAG TCAAAAAGTA CTTATTTTTT 
     2461 GGAGATCACT TCATTCTATT TTCCCTTGCT ATTACCAAAC CAATTGAATT GCGCTGATGC 
     2521 CCAGTTTAAT TTAAATGTTA TTTATTCTGC CTATGAAAAT CGTTTTCACA TTATATGAAA 
     2581 TTGGTCAGAC ATGTTCATTG GTCCTTTGGA AGTGACGTCA TGTCACATCT ATTACCACAA 
     2641 TGCACAGCAC CTTGACCTGG AAATTAGGGA AATTATAACA GTCAATCAGT GGAAGAAAAT 
     2701 GGAGGAAGTA TGTGATTCAT CAGCAGCTGC GAGCAGCACA GTCCAAAATC AGCCACAGGA 
     2761 TCAAGAGCAC CCGTGGCCGT ATCTTCGCAG ATCTGATCTA GAGGATCATA ATCAGCCATA 
     2821 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA 
     2881 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     2941 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     3001 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCATCATC GATGCGTCTT 
     3061 TTGTTCTGGT CATCAAGGAG GGGGGAATGT TCTGCGCATG CCTGTGGGGG AGGGAGAAGG 
     3121 ACACGTCACT GAAAACGTCC CTGCATCACA CCGAGACACC CAATCACTCA AGCCGAGACC 
     3181 AGATAATTTT GCATATGCTT TAAAGTTTGA AAAATACCAC GGTAAACCCT CACACAAACT 
     3241 CTGGATTTGA GATCTTTCAG GTTTTATCAG TTTGCAGGTT TATGTCACCA TGATATAGGG 
     3301 TCAGACTTGA TCTAAGGGAG CTGAATAAGT GGTTTAGTCA CTCACCACCT CCCAAAAACA 
     3361 TACCCAGAAG TCCCTGGTAT ATATAGCTCT CCCTCCAGCT CTTGGTTCGG CTAGCACTCC 
     3421 TCAGTGACGA GGAGTCAGTA GCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG 
     3481 TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTGGTACC GTAAAACGAC 
     3541 GGCCAGTGAA TTATCAACTA TGTATAATAA AGTTGAACGA GAAACGTAAA ATGATATAAA 
     3601 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA 
     3661 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCCA GGCTTTACAC TTTATGCTTC 
     3721 CGGCTCGTAT AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA 
     3781 AGCTAAAATG GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG 
     3841 TAAAGAACAT TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA 
     3901 GCTGGATATT ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC 
     3961 CTTTATTCAC ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA 
     4021 AGACGGTGAG CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA 
     4081 AACTGAAACG TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA 
     4141 CATATATTCG CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT 
     4201 TATTGAGAAT ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT 
     4261 AAACGTGGCC AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGGGCA AATATTATAC 
     4321 GCAAGGCGAC AAGGTGCTGA TGCCGCTGGC GATTCAGGTT CATCATGCCG TTTGTGATGG 
     4381 CTTCCATGTC GGCAGAATGC TTAATGAATT ACAACAGTAC TGCGATGAGT GGCAGGGCGG 
     4441 GGCGTAATCT AGAGGATCCG GCTTACTAAA AGCCAGATAA CAGTATGCGT ATTTGCGCGC 
     4501 TGATTTTTGC GGTATAAGAA TATATACTGA TATGTATACC CGAAGTATGT CAAAAAGAGG 
     4561 TATGCTATGA AGCAGCGTAT TACAGTGACA GTTGACAGCG ACAGCTATCA GTTGCTCAAG 
     4621 GCATATATGA TGTCAATATC TCCGGTTCGG TAAGCACAAC CATGCAGAAT GAAGCCCGTC 
     4681 GTCTGCGTGC CGAACGCTGG AAAGCGGAAA ATCAGGAAGG GATGGCTGAG GTCGCCCGGT 
     4741 TTATTGAAAT GAACGGCTCC TTTGCCGACG AGAACAGGGG CTGGTGAAAT GCAGTTTAAG 
     4801 GTTTACACCT ATAAAAGAGA GAGCCGTTAT CGTCTGTTTG TGGATGTACA GAGTGATATT 
     4861 ATTGACACGC CCGGGCGACG GATGGTGATC CCCCTGGCCA GTGCACGTCT GCTGTCAGAT 
     4921 AAAGTCCCCC GTGAACTTTA CCCGGTGGTG CATATCGGGG ATGAAAGCTG GCGCATGATG 
     4981 ACCACCGATA TGGCCAGTGT GCCGGTCTCC GTTATCGGGG AAGAAGTGGC TGATCTCAGC 
     5041 CACCGCGAAA ATGACATCAA AAACGCCATT AACCTGATGT TCTGGGGAAT ATAAATGTCA 
     5101 GGCTCCCTTA TACACAGCCA GTCTGCAGGT CGACCATAGT GACTGGATAT GTTGTGTTTT 
     5161 ACAGTATTAT GTAGTCTGTT TTTTATGCAA AATCTAATTT AATATATTGA TATTTATATC 
     5221 ATTTTACGTT TCTCGTTCAA CTTTTCTATA CAAAGTTGAT AGCTTGGCGT AATCATGGTC 
     5281 ATAGCTGTTT CCTGCTCGAG GGCCCATCTG GCCTGTGTTT CAGACACCAG GGAGTCTCTG 
     5341 CTCACGTTTC CTGCTATTTG CAGCCTCTCT ATCAAGACTA ATACACCTCT TCCCGCATCG 
     5401 GCTGCCTGTG AGAGGCTTTT CAGCACTGCA GGATTGCTTT TCAGCCCCAA AAGAGCTAGG 
     5461 CTTGACACTA ACAATTTTGA GAATCAGCTT CTACTGAAGT TAAATCTGAG GTTTTACAAC 
     5521 TTTGAGTAGC GTGTACTGGC ATTAGATTGT CTGTCTTATA GTTTGATAAT TAAATACAAA 
     5581 CAGTTCTAAA GCAGGATAAA ACCTTGTATG CATTTCATTT AATGTTTTTT GAGATTAAAA 
     5641 GCTTAAACAA GAATCTCTAG TTTTCTTTCT TGCTTTTACT TTTACTTCCT TAATACTCAA 
     5701 GTACAATTTT AATGGAGTAC TTTTTTACTT TTACTCAAGT AAGATTCTAG CCAGATACTT 
     5761 TTACTTTTAA TTGAGTAAAA TTTTCCCTAA GTACTTGTAC TTTCACTTGA GTAAAATTTT 
     5821 TGAGTACTTT TTACACCTCT GTCAAGAACT CCTGGACAAA CCTCTGACCT GTGTGGAACA 
     5881 GAGTGGATAT GGGTGTCTGA ACAGATATTC ACGTCTTTTG CAGATCAGAG GGCATTTCTG 
     5941 GTGTTGTGCA ATGACCTGGG TCCAACAGGT AGGCGTACTC GTATCCAAGA GCCTTGTTGG 
     6001 ACAGGAGGTA GTCTCCATTC CTATAAAGAG GAAGGAATGG CACCATGTAG TAGCCGTCAT 
     6061 TGTGGCCAAT TGGTGCATTG GTACGTGGGT AGATGGACCG GGGAGGCTGA TGAGTTCTTA 
     6121 GCCAGCGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     6181 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     6241 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     6301 AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG GTGG
//