LOCUS       pRGE32                 10092 bp    DNA     circular     21-FEB-2014
DEFINITION  attB1-attB2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|686024535|
COMMENT     VNTDBDATE|690300455|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRGE32|
COMMENT     VNTAUTHORNAME|Xin H|
FEATURES             Location/Qualifiers
     misc_feature    5717..5737
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(10086..14)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    26..49
                     /vntifkey="21"
                     /label=Misc_Feature_3
                     /note="FLAG"
     terminator      55..331
                     /vntifkey="43"
                     /label=Terminator_1
                     /note="NOS terminator"
     gene            1403..2263
                     /gene="bla"
                     /vntifkey="60"
     CDS             1403..2263
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta lactamase"
                     /protein_id="BAK19544.1"
                     /db_xref="GI:332144745"
                     /vntifkey="4"
                     /label=bla
     rep_origin      complement(2524..3024)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ColE1 Ori"
     promoter        3443..3823
                     /vntifkey="29"
                     /label=Promoter\U3
     prim_transcript 3823..3823
                     /vntifkey="91"
                     /label=start\site
     misc_feature    3840..3915
                     /vntifkey="21"
                     /label=gRNA\scaffold
     misc_marker     3975..3991
                     /vntifkey="22"
                     /label=adaptor
     promoter        3992..5707
                     /vntifkey="29"
                     /label=pUBI10
     exon            5706..5707
                     /vntifkey="61"
                     /label=exon2\start
     primer          3408..3424
                     /vntifkey="27"
                     /label=M13R
     primer          complement(333..348)
                     /vntifkey="27"
                     /label=M13F(-20)
     misc_feature    5902..10002
                     /vntifkey="21"
                     /label=Misc_Feature_2
                     /note="hSpCsn1"
     misc_feature    10003..10050
                     /vntifkey="21"
                     /label=Misc_Feature_4
                     /note="NLS"
     misc_feature    5782..5850
                     /vntifkey="21"
                     /label=Misc_Feature_7
                     /note="3xFLAG"
     misc_feature    5851..5901
                     /vntifkey="21"
                     /label=Misc_Feature_8
                     /note="NLS"
     misc_feature    2119..2120
                     /vntifkey="21"
                     /label=Mut\Bsa\I
BASE COUNT     2732 a      2500 c      2582 g      2278 t 
ORIGIN
        1 cttgtacaaa gtggttgata acagcgacta caaggatgac gatgacaagg cttagagctc 
       61 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc 
      121 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa 
      181 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata 
      241 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc 
      301 ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa cgtcgtgact 
      361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 
      421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 
      481 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 
      541 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
      601 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
      661 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
      721 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg 
      781 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
      841 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
      901 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
      961 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg 
     1021 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 
     1081 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 
     1141 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 
     1201 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 
     1261 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     1321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     1381 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     1441 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     1501 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     1561 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     1621 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     1681 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     1741 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     1801 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     1861 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     1921 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     1981 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     2041 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     2101 tggagccggt gagcgtggca ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     2161 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     2221 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     2281 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     2341 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     2401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     2581 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     2821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     3061 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     3121 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
     3181 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     3241 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     3301 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
     3361 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
     3421 tgaccatgat tacgccaagc ttaaggaatc tttaaacata cgaacagatc acttaaagtt 
     3481 cttctgaagc aacttaaagt tatcaggcat gcatggatct tggaggaatc agatgtgcag 
     3541 tcagggacca tagcacaaga caggcgtctt ctactggtgc taccagcaaa tgctggaagc 
     3601 cgggaacact gggtacgttg gaaaccacgt gatgtgaaga agtaagataa actgtaggag 
     3661 aaaagcattt cgtagtgggc catgaagcct ttcaggacat gtattgcagt atgggccggc 
     3721 ccattacgca attggacgac aacaaagact agtattagta ccacctcggc tatccacata 
     3781 gatcaaagct gatttaaaag agttgtgcag atgatccgtg gcaggagacc gaggtctcgg 
     3841 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg 
     3901 gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt 
     3961 ccgtttttag cgcgtgcatg cctgcaggtc cacaaattcg ggtcaaggcg gaagccagcg 
     4021 cgccacccca cgtcagcaaa tacggaggcg cggggttgac ggcgtcaccc ggtcctaacg 
     4081 gcgaccaaca aaccagccag aagaaattac agtaaaaaaa aagtaaattg cactttgatc 
     4141 caccttttat tacctaagtc tcaatttgga tcacccttaa acctatcttt tcaatttggg 
     4201 ccgggttgtg gtttggacta ccatgaacaa cttttcgtca tgtctaactt ccctttcagc 
     4261 aaacatatga accatatata gaggagatcg gccgtatact agagctgatg tgtttaaggt 
     4321 cgttgattgc acgagaaaaa aaaatccaaa tcgcaacaat agcaaattta tctggttcaa 
     4381 agtgaaaaga tatgtttaaa ggtagtccaa agtaaaactt atagataata aaatgtggtc 
     4441 caaagcgtaa ttcactcaaa aaaaatcaac gagacgtgta ccaaacggag acaaacggca 
     4501 tcttctcgaa atttcccaac cgctcgctcg cccgcctcgt cttcccggaa accgcggtgg 
     4561 tttcagcgtg gcggattctc caagcagacg gagacgtcac ggcacgggac tcctcccacc 
     4621 acccaaccgc cataaatacc agccccctca tctcctctcc tcgcatcagc tccacccccg 
     4681 aaaaatttct ccccaatctc gcgaggctct cgtcgtcgaa tcgaatcctc tcgcgtcctc 
     4741 aaggtacgct gcttctcctc tcctcgcttc gtttcgattc gatttcggac gggtgaggtt 
     4801 gttttgttgc tagatccgat tggtggttag ggttgtcgat gtgattatcg tgagatgttt 
     4861 aggggttgta gatctgatgg ttgtgatttg ggcacggttg gttcgatagg tggaatcgtg 
     4921 gttaggtttt gggattggat gttggttctg atgattgggg ggaattttta cggttagatg 
     4981 aattgttgga tgattcgatt ggggaaatcg gtgtagatct gttggggaat tgtggaacta 
     5041 gtcatgcctg agtgattggt gcgatttgta gcgtgttcca tcttgtaggc cttgttgcga 
     5101 gcatgttcag atctactgtt ccgctcttga ttgagttatt ggtgccatgg gttggtgcaa 
     5161 acacaggctt taatatgtta tatctgtttt gtgtttgatg tagatctgta gggtagttct 
     5221 tcttagacat ggttcaatta tgtagcttgt gcgtttcgat ttgatttcat atgttcacag 
     5281 attagataat gatgaactct tttaattaat tgtcaatggt aaataggaag tcttgtcgct 
     5341 atatctgtca taatgatctc atgttactat ctgccagtaa tttatgctaa gaactatatt 
     5401 agaatatcat gttacaatct gtagtaatat catgttacaa tctgtagttc atctatataa 
     5461 tctattgtgg taatttcttt ttactatctg tgtgaagatt attgccacta gttcattcta 
     5521 cttatttctg aagttcagga tacgtgtgct gttactacct atctgaatac atgtgtgatg 
     5581 tgcctgttac tatctttttg aatacatgta tgttctgttg gaatatgttt gctgtttgat 
     5641 ccgttgttgt gtccttaatc ttgtgctagt tcttacccta tctgtttggt gattatttct 
     5701 tgcagatagt tatcaacaag tttgtacaaa aaagcaggct tcgaaggaga tagaaccaat 
     5761 tctctaagga aatacttaac catggactat aaggaccacg acggagacta caaggatcat 
     5821 gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg gaaggtcggt 
     5881 atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat cggcaccaac 
     5941 tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg 
     6001 ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac 
     6061 agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga 
     6121 cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac 
     6181 gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag 
     6241 cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc 
     6301 atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc 
     6361 tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg 
     6421 aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag 
     6481 ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc 
     6541 agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag 
     6601 aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc 
     6661 aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac 
     6721 ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag 
     6781 aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag 
     6841 gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg 
     6901 ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag 
     6961 agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag 
     7021 ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac 
     7081 agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc 
     7141 cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag 
     7201 gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct 
     7261 ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc 
     7321 ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg 
     7381 atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg 
     7441 tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg 
     7501 agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag 
     7561 accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc 
     7621 ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac 
     7681 cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac 
     7741 attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa 
     7801 cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg 
     7861 agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag 
     7921 tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg 
     7981 cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc 
     8041 ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag 
     8101 aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac 
     8161 aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag 
     8221 aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag 
     8281 atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac 
     8341 tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc 
     8401 gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc catcgacaac 
     8461 aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag 
     8521 gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag 
     8581 agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc 
     8641 ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc 
     8701 ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa 
     8761 gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa 
     8821 gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga 
     8881 accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag 
     8941 gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc 
     9001 aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac 
     9061 ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg 
     9121 gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc 
     9181 gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg 
     9241 aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc 
     9301 gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc 
     9361 aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc 
     9421 gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg 
     9481 atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg 
     9541 gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac 
     9601 ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag 
     9661 aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc 
     9721 gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac 
     9781 aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc 
     9841 ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag 
     9901 aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc 
     9961 ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg 
    10021 aaaaaggccg gccaggcaaa aaagaaaaag taagaattcg cggccgcact cgagatatct 
    10081 agacccagct tt 
//
