LOCUS       Exported                5963 bp ds-DNA     circular SYN 01-DEC-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 01, 2015 from SnapGene 2.8.3
            http://www.snapgene.com
COMMENT     413-RFP loxPsym acceptor vector from 1 to 5959
            RFP BsaI Acceptor Vector pRS413 from 1 to 5878
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    199..498
                     /label=promoter of HIS3
                     /note="promoter of HIS3"
     CDS             499..1161
                     /label=HIS3
                     /note="HIS3"
     misc_feature    join(1162..1302,1320..1358)
                     /label=His3 txn term
                     /note="His3 txn term"
     misc_feature    1303..1319
                     /label=His3 txn term(1)
                     /note="His3 txn term(1)"
     misc_feature    1553..1859
                     /label=FI ori
                     /note="FI ori"
     primer_bind     1994..2017
                     /label=M13F(-47)
                     /note="M13F(-47)"
     primer_bind     2000..2017
                     /label=M13F(-41)
                     /note="M13F(-41)"
     primer_bind     2020..2037
                     /label=M13F(-21)
                     /note="M13F(-21)"
     primer_bind     2047..2066
                     /label=T7
                     /note="T7"
     misc_feature    2111..2144
                     /label=loxPsym
                     /note="loxPsym"
     gene            complement(2182..2204)
                     /label=RFP(3)
                     /note="RFP(3)"
     misc_feature    complement(2224..2901)
                     /label=RFP(4)
                     /note="RFP(4)"
     gene            complement(2902..2925)
                     /label=RFP(2)
                     /note="RFP(2)"
     gene            complement(join(2926..3106,2205..2901))
                     /label=RFP
                     /note="RFP"
     gene            complement(3107..3127)
                     /label=RFP(1)
                     /note="RFP(1)"
     misc_feature    3157..3190
                     /label=loxPsym(1)
                     /note="loxPsym(1)"
     primer_bind     complement(3282..3298)
                     /label=M13R
                     /note="M13R"
     CDS             complement(join(4567..4586,4588..4607))
                     /label=AMPr(1)
                     /note="AMPr(1)"
     CDS             complement(join(4608..5303,4443..4566))
                     /label=AMPr
                     /note="AMPr"
     misc_feature    5435..5952
                     /label=CEN/ARS
                     /note="CEN/ARS"
ORIGIN
        1 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt gtcacagctt
       61 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg cgtgttggcg
      121 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
      181 aattcccgtt ttaagagctt ggtgagcgct aggagtcact gccaggtatc gtttgaacac
      241 ggcattagtc agggaagtca taacacagtc ctttcccgca attttctttt tctattactc
      301 ttggcctcct ctagtacact ctatattttt ttatgcctcg gtaatgattt tcattttttt
      361 ttttccacct agcggatgac tctttttttt tcttagcgat tggcattatc acataatgaa
      421 ttatacatta tataaagtaa tgtgatttct tcgaagaata tactaaaaaa tgagcaggca
      481 agataaacga aggcaaagat gacagagcag aaagccctag taaagcgtat tacaaatgaa
      541 accaagattc agattgcgat ctctttaaag ggtggtcccc tagcgataga gcactcgatc
      601 ttcccagaaa aagaggcaga agcagtagca gaacaggcca cacaatcgca agtgattaac
      661 gtccacacag gtatagggtt tctggaccat atgatacatg ctctggccaa gcattccggc
      721 tggtcgctaa tcgttgagtg cattggtgac ttacacatag acgaccatca caccactgaa
      781 gactgcggga ttgctctcgg tcaagctttt aaagaggccc taggggccgt gcgtggagta
      841 aaaaggtttg gatcaggatt tgcgcctttg gatgaggcac tttccagagc ggtggtagat
      901 ctttcgaaca ggccgtacgc agttgtcgaa cttggtttgc aaagggagaa agtaggagat
      961 ctctcttgcg agatgatccc gcattttctt gaaagctttg cagaggctag cagaattacc
     1021 ctccacgttg attgtctgcg aggcaagaat gatcatcacc gtagtgagag tgcgttcaag
     1081 gctcttgcgg ttgccataag agaagccacc tcgcccaatg gtaccaacga tgttccctcc
     1141 accaaaggtg ttcttatgta gtgacaccga ttatttaaag ctgcagcata cgatatatat
     1201 acatgtgtat atatgtatac ctatgaatgt cagtaagtat gtatacgaac agtatgatac
     1261 tgaagatgac aaggtaatgc atcattctat acgtgtcatt ctgaacgagg cgcgctttcc
     1321 ttttttcttt ttgctttttc tttttttttc tcttgaactc gacggatcta tgcggtgtga
     1381 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1441 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1501 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1561 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1621 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1681 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1741 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1801 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1861 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1921 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1981 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     2041 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
     2101 ggcagctggg ataacttcgt ataatgtaca ttatacgaag ttatgggacg tggcgaattc
     2161 ctgcagcccc cagtagagac cgcctggctc tagtagcgat ctacactagc actatcagcg
     2221 ttattaagca ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta
     2281 gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg
     2341 aaccggtttt ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt
     2401 cagtttcaga cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc
     2461 ggtggaagct tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt
     2521 ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt
     2581 aacggtaaca acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg
     2641 gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc
     2701 gtactggaac tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac
     2761 tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc
     2821 gaactcgtga ccgttaacgg aaccttccat acgaactttg aaacgcatga actctttgat
     2881 aacgtcttcg gaggaagcca tctagtattt ctcctctttc tctagtatgt gtgaaattgt
     2941 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3001 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3061 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3121 cgtattgggt ctcattttgg ggatcccgga gcacccataa cttcgtataa tgtacattat
     3181 acgaagttat cccaggtcgc tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     3241 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     3301 tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa
     3361 gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct
     3421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4561 cccagtgctg caatgatacc gcgagatcca cgctcaccgg ctccagattt atcagcaata
     4621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5401 gttccgcgca catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa
     5461 ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa
     5521 gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc
     5581 caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt
     5641 catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg
     5701 ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt
     5761 acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc
     5821 ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac
     5881 agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc
     5941 aagcgatccg tcctaagaaa cca
//
