LOCUS       pQE_WT_N4_R5_YRN        7154 bp    DNA     circular UNA 04-JUN-2015
DEFINITION  .
ACCESSION   urn.local...i-4r1afll
VERSION     urn.local...i-4r1afll
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        8..94
                     /created_by="User"
                     /label="T5 promoter/ Lac operator"
     operator        30..48
                     /created_by="User"
                     /label="lac operator"
     TSS             61
                     /created_by="User"
                     /label="+1 "
     operator        62..81
                     /created_by="User"
                     /label="lac operator"
     RBS             95..107
                     /created_by="User"
                     /label="RBS"
     ORF             115..2799
                     /Original_Bases="GCATGCGAGCTCGGTACCCCGGGTCGACCTGCAGCC"
                     /modified_by="User"
                     /label="T7 WT"
     primer          765..787
                     /created_by="User"
                     /label="AJM.T7.IntrnlSeq.2"
     primer          865..889
                     /created_by="User"
                     /modified_by="User"
                     /label="AJM.T7WTseq2.1.F"
     primer          complement(2132..2175)
                     /created_by="User"
                     /modified_by="User"
                     /label="AJM.Y.WTR"
     primer          2139..2180
                     /created_by="User"
                     /modified_by="User"
                     /label="AJM.Y.WTF"
     misc_feature    2158
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     primer          complement(2386..2445)
                     /created_by="User"
                     /modified_by="User"
                     /label="AJM.N4RNRV.WTR"
     misc_feature    2411
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     primer          2417..2476
                     /created_by="User"
                     /modified_by="User"
                     /label="AJM.N4RNRV.WTF"
     misc_feature    2455
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2462
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2471
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     ORF             2959..3618
                     /created_by="User"
                     /modified_by="User"
                     /label="KanR"
     terminator      3686..3729
                     /label="rrnB_T1_terminator"
     ORF             complement(3862..4953)
                     /created_by="User"
                     /modified_by="User"
                     /label="lacI"
     ori             5525..6144
                     /created_by="User"
                     /label="pBR322"
     primer          complement(5942..6001)
                     /created_by="User"
                     /label="AJM.pBR322.Gib.F"
     primer          6174..6203
                     /created_by="User"
                     /label="AJM.pQE.ISOF.b"
     primer          complement(6174..6203)
                     /created_by="User"
                     /label="AJM.pQE.ISOR.b"
ORIGIN      
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc aacacgatta acatcgctaa gaacgacttc
      181 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt
      241 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc
      301 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag
      361 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa
      421 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg
      481 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat
      541 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc
      601 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac
      661 aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc
      721 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat
      781 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc
      841 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct
      901 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc
      961 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt
     1021 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt
     1081 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac
     1141 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac
     1201 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct
     1261 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc
     1321 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat
     1381 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg
     1441 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca
     1501 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat
     1561 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct
     1621 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc
     1681 cttgcattct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc
     1741 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga
     1801 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac
     1861 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat
     1921 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg
     1981 ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag
     2041 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg
     2101 gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgtat
     2161 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct
     2221 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa
     2281 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat
     2341 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcattga ctgcgtgatc
     2401 ctcggtaccc gtcgcatggc gctgaccatt aacaccaaca aagatagcga gattaatgca
     2461 cgcaaacagg tgtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt
     2521 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac
     2581 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact
     2641 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac
     2701 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac
     2761 ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaaa agcttaatta gctgagcttg
     2821 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     2881 tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagcttggc gagattttca
     2941 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     3001 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     3061 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     3121 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
     3181 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     3241 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     3301 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     3361 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     3421 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     3481 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     3541 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     3601 tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggggta
     3661 atgactctct agcttgaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     3721 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccctctag
     3781 attacgtgca gtcgatgata agctgtcaaa catgagaatt gtgcctaatg agtgagctaa
     3841 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     3961 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     4021 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     4081 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     4141 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     4201 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     4261 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     4321 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     4381 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     4441 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     4501 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     4561 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     4621 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     4681 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     4741 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     4801 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     4861 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     4921 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     4981 tcatgccata ccgcgaaagg ttttgcacca ttcgatggtg tcggaatttc gggcagcgtt
     5041 gggtcctggc cacgggtgcg catgatctag agctgcctcg cgcgtttcgg tgatgacggt
     5101 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     5161 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     5221 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     5281 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     5341 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5401 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5461 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5521 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5581 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5641 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5701 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5761 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5881 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5941 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     6001 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6061 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6121 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6181 cacgttaagg gattttggtc atggcgttaa gggattttgg tcatgaatta attcttagaa
     6241 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     6301 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     6361 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     6421 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     6481 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     6541 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     6601 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     6661 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     6721 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     6781 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     6841 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     6901 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     6961 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     7021 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     7081 agacagtttt attgttcatg accatgacat taacctataa aaataggcgt atcacgaggc
     7141 cctttcgtct tcac
//
