LOCUS       p2989_(pTY1Cons1-KlURA3-deg)      5472 bp    DNA     circular UNA 
FEATURES             Location/Qualifiers
     Misc._feature   1..14
                     /label="USER cassette"
     Terminator      20..190
                     /vntifkey="43"
                     /label="T CYC1"
                     /label="T CYC1"
     Misc._feature   257..316
                     /vntifkey="21"
                     /label=loxP
                     /note="Unknown feature type:Misc._feature"
     Terminator      complement(317..439)
                     /label="T URA3"
                     /label="T URA3"
     Misc._structure complement(440..490)
                     /vntifkey="88"
                     /label="Degradation tag"
                     /note="Unknown feature type:Misc._structure"
                     /label="Degradation tag"
     CDS             complement(491..1291)
                     /gene="URA3"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="AAG34531.1"
                     /db_xref="GI:11344893"
                     /vntifkey="4"
                     /label="K. lactis URA3"
                     /label="K. lactis URA3syn without STOP codon"
                     /label="K. l. URA3"
     Promoter        complement(1292..1790)
                     /vntifkey="29"
                     /label="K. lactis URA3 promoter"
                     /label="P K. l. URA3"
     Misc._feature   1807..1840
                     /vntifkey="21"
                     /label=loxP
     Misc._feature   1980..2187
                     /vntifkey="21"
                     /label="TY1 C1 3'"
                     /note="Unknown feature type:Misc._feature"
                     /label="TY1 C1 3'"
                     /label="Consensus TY1Cons1 3'"
     Replication_ori complement(2960..3603)
                     /vntifkey="33"
                     /label="pUC ori"
                     /note="Unknown feature type:Replication_ori"
                     /label="pUC ori"
     CDS             complement(3706..4564)
                     /vntifkey="4"
                     /label=AmpR
     Misc._feature   5008..5241
                     /vntifkey="21"
                     /label="TY1 C1 5'"
                     /note="Unknown feature type:Misc._feature"
                     /label="TY1 C1 5'"
                     /label="Consensus TY1Cons1 5'"
     Terminator      complement(5268..5462)
                     /vntifkey="43"
                     /label="T ADH1"
                     /label="T ADH1"
ORIGIN
        1 CGTGCGATCG CGTGCATTCA TCCGCTCTAA CCGAAAAGGA AGGAGTTAGA CAACCTGAAG
       61 TCTAGGTCCC TATTTATTTT TTTATAGTTA TGTTAGTATT AAGAACGTTA TTTATATTTC
      121 AAATTTTTCT TTTTTTTCTG TACAGACGCG TGTACGCATG TAACATTATA CTGAAAACCT
      181 TGCTTGAGAA GGTTTTGGGA CGCTCGAAGA TCGCGTCAGC TGAAGCTTCG TACGCTGCAG
      241 GTCGACAACC CTTAATGTCG ACAACCCTTA ATATAACTTC GTATAATGTA TGCTATACGA
      301 AGTTATTAGG TCTAGAGATC CCAATACAAC AGATCACGTG ATCTTTTGTA AGATGAAGTT
      361 GAAGTGAGTG TTGCACCGTG CCAATGCAGG TGGCTATTAG ATTAAATATG TGATTTGTTC
      421 TATTAAGTTT CCTGTATAAT TACAAATGAA TAACGAAATG AGACAAAGAA GAGAACCAAU
      481 TTTTACAAGC ATGGGGAGCG CTGATTCTCT TTTGGTACGC TTCCCATCCA GCATTTCTGT
      541 ATCTTTCACC TTCAACCTTA GGATCTCTAC CCTTGGCGAA AAGTCCTCTG CCAACAATGA
      601 TGATATCTGA TCCACCACTT ACAACTTCGT CGACGGTTCT GTACTGCTGA CCCAATGCAT
      661 CGCCTTTGTC GTCTAAACCT ACACCTGGGG TCATGATTAG CCAATCAAAC CCTTCTTCTC
      721 TTCCTCCCAT ATCGTTCTGA GCAATGAACC CAATAACGAA ATCTTTATCA CTCTTTGCAA
      781 TATCAACGGT ACCCTTAGTA TATTCACCGT GTGCTAGAGA ACCCTTGGAA GATAATTCAG
      841 CAAGCATCAA TAATCCCCTT GGTTCTTTGG TGACCTCTTG CGCACCTTGT TTCAAGCCAG
      901 CAACAATACC AGCACCAGTA ACCCCGTGGG CGTTGGTGAT ATCAGACCAT TCTGCGATAC
      961 GGTAAACGCC CGATGTATAT TGTAATTTGA CTGTGTTACC GATATCGGCG AATTTTCTGT
     1021 CCTCAAATAT CAAGAACTTG TATTTCTCTG CCAATGCTTT CAATGGAACG ACAGTACCCT
     1081 CATAACTGAA ATCATCCAAG ATATCAACGT GTGTTTTCAA AAGGCAAATG TATGGACCCA
     1141 ACGTTTCAAC AAGTTTCAAT AGCTCATCAG TCGAACGAAC GTCAAGAGAA GCACACAAAT
     1201 TGGTTTTCTT TTCATCCATT AAACGTAAAA GTTTCGATGC AACCGGACTT GCATGAGTCT
     1261 CAGCTCTACT GGTATATGAT TTTGTGGACA TGGTGCAACT AATTGACGGG AGTGTATTGA
     1321 CGCTGGCGTA CTGGCTTTCA CAAAATGGCC CAATCACAAC CACATCTTAG ATAGTTGAAA
     1381 TGACTTTAGA TAACATCAAT TGAGATGAGC TTAATCATGT CAAAGCTAAA AGTGTCACCA
     1441 TGAACGACAA TTCTTAAGCA AATCACGTGA TATAGATCCA CGAATAACCA CCATTTGATG
     1501 CTCGAGGCAA GTAATGTGTG TAAAAAAATG CGTTACCACC ATCCAATGCA GACCGATCTT
     1561 CTACCCAGAA TCACATATAT TTATGTACCG AGTACCTTTT TTCTATCTTC CAATTGCTTC
     1621 TCCCATATGA TTGTCTCCGT AAGCTCGAAA TTTCTAAGTT GGATTTTAAT CTTCACGCAG
     1681 GATGACAGTT CGATGAGCTT CTGAGGAGTG TTTAGAACAT AATCAGTTTA TCCATGGTCT
     1741 ATCTCTTCTT GTCGCTTTTT CTCCTCGATA GAACCTAAAT AAAACGAGCT CTCGAGAACC
     1801 CTTAATATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTGATAT CAGATCCACT
     1861 AGTGGCCTAT GCACCCAATT CGCCCTATAG TGAGTCGTAT TACGCGCGCT CACTGGCCGT
     1921 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CCTGCAGGAC AGACGTCATT
     1981 AGTGCTGAGG CATTAATTGA TCAAAAACGG AATGAGGAAT AATCGTAATA TTAGTATGTA
     2041 GAAATATAGA TTCCATTTTG AGGATTCCTA TATCCTCGAG GAGAACTTCT AGTGTATATT
     2101 CTGTATACCT AATATTATAG CCTTTATCAA CAATGGAATC CCAACAATTA TCTAATTACC
     2161 CACAAATTTC TCAAGATCTG CGGCCGCACT CAGACCTGAA GTGAAGTTCC TATACTTTCT
     2221 AGAGAATAGG AACTTCTATA GTGAGTCGAA TAAGGGCGAC ACAAAATTTA TTCTAAATGC
     2281 ATAATAAATA CTGATAACAT CTTATAGTTT GTATTATATT TTGTATTATC GTTGACATGT
     2341 ATAATTTTGA TATCAAAAAC TGATTTTCCC TTTATTATTT TCGAGATTTA TTTTCTTAAT
     2401 TCTCTTTAAC AAACTAGAAA TATTGTATAT ACAAAAAATC ATAAATAATA GATGAATAGT
     2461 TTAATTATAG GTGTTCATCA ATCGAAAAAG CAACGTATCT TATTTAAAGT GCGTTGCTTT
     2521 TTTCTCATTT ATAAGGTTAA ATAATTCTCA TATATCAAGC AAAGTGACAG GCGCCCTTAA
     2581 ATATTCTGAC AAATGCTCTT TCCCTAAACT CCCCCCATAA AAAAACCCGC CGAAGCGGGT
     2641 TTTTACGTTA TTTGCGGATT AACGATTACT CGTTATCAGA ACCGCCCAGG GGGCCCGAGC
     2701 TTAAGACTGG CCGTCGTTTT ACAACACAGA AAGAGTTTGT AGAAACGCAA AAAGGCCATC
     2761 CGTCAGGGGC CTTCTGCTTA GTTTGATGCC TGGCAGTTCC CTACTCTCGC CTTCCGCTTC
     2821 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
     2881 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
     2941 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
     3001 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     3061 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     3121 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     3181 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     3241 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     3301 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     3361 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGG CTAACTACGG
     3421 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     3481 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     3541 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     3601 TACGGGGTCT GACGCTCAGT GGAACGACGC GCGCGTAACT CACGTTAAGG GATTTTGGTC
     3661 ATGAGCTTGC GCCGTCCCGT CAAGTCAGCG TAATGCTCTG CTTTTACCAA TGCTTAATCA
     3721 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
     3781 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGCGCT GCGATGATAC
     3841 CGCGAGAACC ACGCTCACCG GCTCCGGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
     3901 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
     3961 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATCGCTA
     4021 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
     4081 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
     4141 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
     4201 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
     4261 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
     4321 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
     4381 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
     4441 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
     4501 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
     4561 TCATATTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG
     4621 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTCAGTGTT ACAACCAATT
     4681 AACCAATTCT GAACATTATC GCGAGCCCAT TTATACCTGA ATATGGCTCA TAACACCCCT
     4741 TGTTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG
     4801 AAACGCCGTA GCGCCGATGG TAGTGTGGGG ACTCCCCATG CGAGAGTAGG GAACTGCCAG
     4861 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGCCCGG GCTAATTATG
     4921 GGGTGTCGCC CTTATTCGAC TCTATAGTGA AGTTCCTATT CTCTAGAAAG TATAGGAACT
     4981 TCTGAAGTGG GGATTTAAAG TCGGTGTCCG CGCTGAGGGT TTAATGGCGC GCCGCGGCCG
     5041 CCCGCGGTGT TGGAATAAAA ATCCACTATC GTCTATCAAC TAATAGTTAT ATTATCAATA
     5101 TATTATCATA TACGGTGTTA AGATGATGAC ATAAGTTATG AGAAGCTGTC ATCGAAGTTA
     5161 GAGGAAGCTG AAGTGCAAGG ATTGATAATG TAATAGGATC AATGAATATA AACATATGCT
     5221 AGCATTAAGT CCTCAGCGAG CACGCTGCTT CATGGAATGC GTGCGATGAG CGACCTCATG
     5281 CTATACCTGA GAAAGCAACC TGACCTACAG GAAAGAGTTA CTCAAGAATA AGAATTTTCG
     5341 TTTTAAAACC TAAGAGTCAC TTTAAAATTT GTATACACTT ATTTTTTTTA TAACTTATTT
     5401 AATAATAAAA ATCATAAATC ATAAGAAATT CGCTTATTTA GAAGTGTCAA CAACGTATCT
     5461 ACCAACGGAA TG
//
