LOCUS       p2875_(pTY3-KlURA3-deg)      5473 bp    DNA     circular UNA 
FEATURES             Location/Qualifiers
     Misc._feature   1..14
                     /label="USER cassette"
     Terminator      20..190
                     /vntifkey="43"
                     /label="T CYC1"
                     /label="T CYC1"
     Misc._feature   257..316
                     /vntifkey="21"
                     /label=loxP
                     /note="Unknown feature type:Misc._feature"
     Terminator      complement(317..439)
                     /label="T URA3"
                     /label="T URA3"
     Misc._structure complement(440..490)
                     /vntifkey="88"
                     /label="Degradation tag"
                     /note="Unknown feature type:Misc._structure"
                     /label="Degradation tag"
     CDS             complement(491..1291)
                     /gene="URA3"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="AAG34531.1"
                     /db_xref="GI:11344893"
                     /vntifkey="4"
                     /label="K. lactis URA3"
                     /label="K. lactis URA3syn without STOP codon"
                     /label="K. l. URA3"
     Promoter        complement(1292..1790)
                     /vntifkey="29"
                     /label="K. lactis URA3 promoter"
                     /label="P K.l. URA3"
     Misc._feature   1807..1840
                     /vntifkey="21"
                     /label=loxP
     Misc._feature   1980..2180
                     /vntifkey="21"
                     /label="TY3 3'"
                     /note="Unknown feature type:Misc._feature"
                     /label="TY3 3'"
                     /label="Consensus TY3 3'"
     Replication_ori complement(2961..3604)
                     /vntifkey="33"
                     /label="pUC ori"
                     /note="Unknown feature type:Replication_ori"
                     /label="pUC ori"
     CDS             complement(3707..4565)
                     /vntifkey="4"
                     /label=AmpR
     Misc._feature   5009..5242
                     /vntifkey="21"
                     /label="TY3 5'"
                     /note="Unknown feature type:Misc._feature"
                     /label="TY3 5'"
                     /label="Consensus TY3 5'"
     Terminator      complement(5269..5463)
                     /vntifkey="43"
                     /label="T ADH1"
                     /label="T ADH1"
ORIGIN
        1 CGTGCGATCG CGTGCATTCA TCCGCTCTAA CCGAAAAGGA AGGAGTTAGA CAACCTGAAG
       61 TCTAGGTCCC TATTTATTTT TTTATAGTTA TGTTAGTATT AAGAACGTTA TTTATATTTC
      121 AAATTTTTCT TTTTTTTCTG TACAGACGCG TGTACGCATG TAACATTATA CTGAAAACCT
      181 TGCTTGAGAA GGTTTTGGGA CGCTCGAAGA TCGCGTCAGC TGAAGCTTCG TACGCTGCAG
      241 GTCGACAACC CTTAATGTCG ACAACCCTTA ATATAACTTC GTATAATGTA TGCTATACGA
      301 AGTTATTAGG TCTAGAGATC CCAATACAAC AGATCACGTG ATCTTTTGTA AGATGAAGTT
      361 GAAGTGAGTG TTGCACCGTG CCAATGCAGG TGGCTATTAG ATTAAATATG TGATTTGTTC
      421 TATTAAGTTT CCTGTATAAT TACAAATGAA TAACGAAATG AGACAAAGAA GAGAACCAAU
      481 TTTTACAAGC ATGGGGAGCG CTGATTCTCT TTTGGTACGC TTCCCATCCA GCATTTCTGT
      541 ATCTTTCACC TTCAACCTTA GGATCTCTAC CCTTGGCGAA AAGTCCTCTG CCAACAATGA
      601 TGATATCTGA TCCACCACTT ACAACTTCGT CGACGGTTCT GTACTGCTGA CCCAATGCAT
      661 CGCCTTTGTC GTCTAAACCT ACACCTGGGG TCATGATTAG CCAATCAAAC CCTTCTTCTC
      721 TTCCTCCCAT ATCGTTCTGA GCAATGAACC CAATAACGAA ATCTTTATCA CTCTTTGCAA
      781 TATCAACGGT ACCCTTAGTA TATTCACCGT GTGCTAGAGA ACCCTTGGAA GATAATTCAG
      841 CAAGCATCAA TAATCCCCTT GGTTCTTTGG TGACCTCTTG CGCACCTTGT TTCAAGCCAG
      901 CAACAATACC AGCACCAGTA ACCCCGTGGG CGTTGGTGAT ATCAGACCAT TCTGCGATAC
      961 GGTAAACGCC CGATGTATAT TGTAATTTGA CTGTGTTACC GATATCGGCG AATTTTCTGT
     1021 CCTCAAATAT CAAGAACTTG TATTTCTCTG CCAATGCTTT CAATGGAACG ACAGTACCCT
     1081 CATAACTGAA ATCATCCAAG ATATCAACGT GTGTTTTCAA AAGGCAAATG TATGGACCCA
     1141 ACGTTTCAAC AAGTTTCAAT AGCTCATCAG TCGAACGAAC GTCAAGAGAA GCACACAAAT
     1201 TGGTTTTCTT TTCATCCATT AAACGTAAAA GTTTCGATGC AACCGGACTT GCATGAGTCT
     1261 CAGCTCTACT GGTATATGAT TTTGTGGACA TGGTGCAACT AATTGACGGG AGTGTATTGA
     1321 CGCTGGCGTA CTGGCTTTCA CAAAATGGCC CAATCACAAC CACATCTTAG ATAGTTGAAA
     1381 TGACTTTAGA TAACATCAAT TGAGATGAGC TTAATCATGT CAAAGCTAAA AGTGTCACCA
     1441 TGAACGACAA TTCTTAAGCA AATCACGTGA TATAGATCCA CGAATAACCA CCATTTGATG
     1501 CTCGAGGCAA GTAATGTGTG TAAAAAAATG CGTTACCACC ATCCAATGCA GACCGATCTT
     1561 CTACCCAGAA TCACATATAT TTATGTACCG AGTACCTTTT TTCTATCTTC CAATTGCTTC
     1621 TCCCATATGA TTGTCTCCGT AAGCTCGAAA TTTCTAAGTT GGATTTTAAT CTTCACGCAG
     1681 GATGACAGTT CGATGAGCTT CTGAGGAGTG TTTAGAACAT AATCAGTTTA TCCATGGTCT
     1741 ATCTCTTCTT GTCGCTTTTT CTCCTCGATA GAACCTAAAT AAAACGAGCT CTCGAGAACC
     1801 CTTAATATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTGATAT CAGATCCACT
     1861 AGTGGCCTAT GCACCCAATT CGCCCTATAG TGAGTCGTAT TACGCGCGCT CACTGGCCGT
     1921 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CCTGCAGGAC AGACGTCATT
     1981 AGTGCTGAGG CATTAATTGA TCAGATTCCG CGCTTCCACC ACTTAGTATG ATTCATATTT
     2041 TATATAATAT ATAAGATAAG TAACATTCCG TGAATTAATC TGATAAACTG TTTTGACAAC
     2101 TGGTTACTTC CCTAAGACTG TTTATATTAG GATTGTCAAG ACACTCCGGT ATTACTCGAG
     2161 CCCGTAATAC AACAAGATCT GCGGCCGCAC TCAGACCTGA AGTGAAGTTC CTATACTTTC
     2221 TAGAGAATAG GAACTTCTAT AGTGAGTCGA ATAAGGGCGA CACAAAATTT ATTCTAAATG
     2281 CATAATAAAT ACTGATAACA TCTTATAGTT TGTATTATAT TTTGTATTAT CGTTGACATG
     2341 TATAATTTTG ATATCAAAAA CTGATTTTCC CTTTATTATT TTCGAGATTT ATTTTCTTAA
     2401 TTCTCTTTAA CAAACTAGAA ATATTGTATA TACAAAAAAT CATAAATAAT AGATGAATAG
     2461 TTTAATTATA GGTGTTCATC AATCGAAAAA GCAACGTATC TTATTTAAAG TGCGTTGCTT
     2521 TTTTCTCATT TATAAGGTTA AATAATTCTC ATATATCAAG CAAAGTGACA GGCGCCCTTA
     2581 AATATTCTGA CAAATGCTCT TTCCCTAAAC TCCCCCCATA AAAAAACCCG CCGAAGCGGG
     2641 TTTTTACGTT ATTTGCGGAT TAACGATTAC TCGTTATCAG AACCGCCCAG GGGGCCCGAG
     2701 CTTAAGACTG GCCGTCGTTT TACAACACAG AAAGAGTTTG TAGAAACGCA AAAAGGCCAT
     2761 CCGTCAGGGG CCTTCTGCTT AGTTTGATGC CTGGCAGTTC CCTACTCTCG CCTTCCGCTT
     2821 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     2881 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     2941 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     3001 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     3061 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     3121 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     3181 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     3241 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     3301 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     3361 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG GCTAACTACG
     3421 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     3481 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     3541 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     3601 CTACGGGGTC TGACGCTCAG TGGAACGACG CGCGCGTAAC TCACGTTAAG GGATTTTGGT
     3661 CATGAGCTTG CGCCGTCCCG TCAAGTCAGC GTAATGCTCT GCTTTTACCA ATGCTTAATC
     3721 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     3781 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGCGC TGCGATGATA
     3841 CCGCGAGAAC CACGCTCACC GGCTCCGGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     3901 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     3961 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATCGCT
     4021 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     4081 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     4141 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     4201 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     4261 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     4321 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     4381 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     4441 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     4501 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     4561 CTCATATTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     4621 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCAGTGT TACAACCAAT
     4681 TAACCAATTC TGAACATTAT CGCGAGCCCA TTTATACCTG AATATGGCTC ATAACACCCC
     4741 TTGTTTGCCT GGCGGCAGTA GCGCGGTGGT CCCACCTGAC CCCATGCCGA ACTCAGAAGT
     4801 GAAACGCCGT AGCGCCGATG GTAGTGTGGG GACTCCCCAT GCGAGAGTAG GGAACTGCCA
     4861 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGCCCG GGCTAATTAT
     4921 GGGGTGTCGC CCTTATTCGA CTCTATAGTG AAGTTCCTAT TCTCTAGAAA GTATAGGAAC
     4981 TTCTGAAGTG GGGATTTAAA GTCGGTGTCC GCGCTGAGGG TTTAATGGCG CGCCGCGGCC
     5041 GCCCGCGGTG TTGTATCTCA AAATGAGATA TGTCAGTATG ACAATACGTC ATCCTGAACG
     5101 TTCATAAAAC ACATATGAAA CAACCTTATA ACAAAACGAA CAACATGAGA CAAAACCCGT
     5161 CCTTCCCTAG CTGAACTACC CAAAAGTATA AATGCCTGAA CAATTAGTTT AGATCCGAGC
     5221 TAGCATTAAG TCCTCAGCGA GCACGCTGCT TCATGGAATG CGTGCGATGA GCGACCTCAT
     5281 GCTATACCTG AGAAAGCAAC CTGACCTACA GGAAAGAGTT ACTCAAGAAT AAGAATTTTC
     5341 GTTTTAAAAC CTAAGAGTCA CTTTAAAATT TGTATACACT TATTTTTTTT ATAACTTATT
     5401 TAATAATAAA AATCATAAAT CATAAGAAAT TCGCTTATTT AGAAGTGTCA ACAACGTATC
     5461 TACCAACGGA ATG
//
