LOCUS       px330-U6-gRNA-CIB1-dCas9        8986 bp    DNA     circular     12-JUN-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|700532033|
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                     /label=CMV
     misc_feature    8818..109
                     /vntifkey="21"
                     /label=CAG-Enhancer
     misc_feature    7118..8048
                     /vntifkey="21"
                     /label=AMP
     misc_feature    484..507
                     /vntifkey="21"
                     /label=NLS
     misc_feature    412..477
                     /vntifkey="21"
                     /label=3xFLAG
     misc_feature    1507..5610
                     /vntifkey="21"
                     /label=dCas9
     misc_feature    541..1458
                     /vntifkey="21"
                     /label=CIB1
     CDS             8352..8751
                     /vntifkey="4"
                     /label=px330-U6-gRNA
BASE COUNT     2663 a      1975 c      2169 g      2179 t 
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggactacaaa 
      421 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagatg 
      481 gcccccaaga agaagaggaa ggtgggcatt caccgcgggg tacctggagg ttctggatcc 
      541 aacggcgcga ttggtgggga tttgctgctt aactttcccg acatgtccgt gttggaacgt 
      601 cagcgcgcac atttgaagta tcttaacccc accttcgact ccccgttggc cgggttcttt 
      661 gcggactcat ctatgattac gggaggggaa atggacagct acctctcaac ggccggattg 
      721 aatcttccga tgatgtatgg agaaaccact gtagaaggcg actcgcgact ctcgatttcg 
      781 cctgaaacga cgctgggaac agggaacttc aaggcggcga aattcgacac ggagacaaaa 
      841 gattgcaacg aagcggcgaa gaaaatgacc atgaatcgcg atgatctggt agaggaggga 
      901 gaggaggaaa agtcgaagat tactgaacag aacaatgggt ctaccaaaag tatcaaaaag 
      961 atgaagcaca aagctaagaa agaagagaac aatttcagca atgacagcag taaagtcaca 
     1021 aaagaactgg agaaaacgga ttacattcac gtgagggcgc gacgagggca ggctacagat 
     1081 tcacattcaa ttgcggagag agtacggaga gagaaaatct cagaaaggat gaagttcctc 
     1141 caagaccttg tgccaggttg tgacaagatc acaggcaaag caggaatgct ggatgagatc 
     1201 atcaactacg tccaatcgtt gcaaagacaa attgagtttc tctcgatgaa actggccatc 
     1261 gtgaatccta gaccggattt cgacatggat gacatctttg cgaaagaagt ggcatccact 
     1321 cccatgacgg ttgtgccctc accggagatg gtcttgtctg gttacagcca cgaaatggtg 
     1381 cattcgggtt attcaagcga gatggtcaat tcgggatacc ttcacgtcaa tcccatgcag 
     1441 caggtgaata cttccagtag cggcagcgag actcccggga cctcagagtc cgccacaccc 
     1501 gaaagtgata aaaagtattc tattggttta gctatcggca ctaattccgt tggatgggct 
     1561 gtcataaccg atgaatacaa agtaccttca aagaaattta aggtgttggg gaacacagac 
     1621 cgtcattcga ttaaaaagaa tcttatcggt gccctcctat tcgatagtgg cgaaacggca 
     1681 gaggcgactc gcctgaaacg aaccgctcgg agaaggtata cacgtcgcaa gaaccgaata 
     1741 tgttacttac aagaaatttt tagcaatgag atggccaaag ttgacgattc tttctttcac 
     1801 cgtttggaag agtccttcct tgtcgaagag gacaagaaac atgaacggca ccccatcttt 
     1861 ggaaacatag tagatgaggt ggcatatcat gaaaagtacc caacgattta tcacctcaga 
     1921 aaaaagctag ttgactcaac tgataaagcg gacctgaggt taatctactt ggctcttgcc 
     1981 catatgataa agttccgtgg gcactttctc attgagggtg atctaaatcc ggacaactcg 
     2041 gatgtcgaca aactgttcat ccagttagta caaacctata atcagttgtt tgaagagaac 
     2101 cctataaatg caagtggcgt ggatgcgaag gctattctta gcgcccgcct ctctaaatcc 
     2161 cgacggctag aaaacctgat cgcacaatta cccggagaga agaaaaatgg gttgttcggt 
     2221 aaccttatag cgctctcact aggcctgaca ccaaatttta agtcgaactt cgacttagct 
     2281 gaagatgcca aattgcagct tagtaaggac acgtacgatg acgatctcga caatctactg 
     2341 gcacaaattg gagatcagta tgcggactta tttttggctg ccaaaaacct tagcgatgca 
     2401 atcctcctat ctgacatact gagagttaat actgagatta ccaaggcgcc gttatccgct 
     2461 tcaatgatca aaaggtacga tgaacatcac caagacttga cacttctcaa ggccctagtc 
     2521 cgtcagcaac tgcctgagaa atataaggaa atattctttg atcagtcgaa aaacgggtac 
     2581 gcaggttata ttgacggcgg agcgagtcaa gaggaattct acaagtttat caaacccata 
     2641 ttagagaaga tggatgggac ggaagagttg cttgtaaaac tcaatcgcga agatctactg 
     2701 cgaaagcagc ggactttcga caacggtagc attccacatc aaatccactt aggcgaattg 
     2761 catgctatac ttagaaggca ggaggatttt tatccgttcc tcaaagacaa tcgtgaaaag 
     2821 attgagaaaa tcctaacctt tcgcatacct tactatgtgg gacccctggc ccgagggaac 
     2881 tctcggttcg catggatgac aagaaagtcc gaagaaacga ttactccatg gaattttgag 
     2941 gaagttgtcg ataaaggtgc gtcagctcaa tcgttcatcg agaggatgac caactttgac 
     3001 aagaatttac cgaacgaaaa agtattgcct aagcacagtt tactttacga gtatttcaca 
     3061 gtgtacaatg aactcacgaa agttaagtat gtcactgagg gcatgcgtaa acccgccttt 
     3121 ctaagcggag aacagaagaa agcaatagta gatctgttat tcaagaccaa ccgcaaagtg 
     3181 acagttaagc aattgaaaga ggactacttt aagaaaattg aatgcttcga ttctgtcgag 
     3241 atctccgggg tagaagatcg atttaatgcg tcacttggta cgtatcatga cctcctaaag 
     3301 ataattaaag ataaggactt cctggataac gaagagaatg aagatatctt agaagatata 
     3361 gtgttgactc ttaccctctt tgaagatcgg gaaatgattg aggaaagact aaaaacatac 
     3421 gctcacctgt tcgacgataa ggttatgaaa cagttaaaga ggcgtcgcta tacgggctgg 
     3481 ggacgattgt cgcggaaact tatcaacggg ataagagaca agcaaagtgg taaaactatt 
     3541 ctcgattttc taaagagcga cggcttcgcc aataggaact ttatgcagct gatccatgat 
     3601 gactctttaa ccttcaaaga ggatatacaa aaggcacagg tttccggaca aggggactca 
     3661 ttgcacgaac atattgcgaa tcttgctggt tcgccagcca tcaaaaaggg catactccag 
     3721 acagtcaaag tagtggatga gctagttaag gtcatgggac gtcacaaacc ggaaaacatt 
     3781 gtaatcgaga tggcacgcga aaatcaaacg actcagaagg ggcaaaaaaa cagtcgagag 
     3841 cggatgaaga gaatagaaga gggtattaaa gaactgggca gccagatctt aaaggagcat 
     3901 cctgtggaaa atacccaatt gcagaacgag aaactttacc tctattacct acaaaatgga 
     3961 agggacatgt atgttgatca ggaactggac ataaaccgtt tatctgatta cgacgtcgat 
     4021 gccattgtac cccaatcctt tttgaaggac gattcaatcg acaataaagt gcttacacgc 
     4081 tcggataaga accgagggaa aagtgacaat gttccaagcg aggaagtcgt aaagaaaatg 
     4141 aagaactatt ggcggcagct cctaaatgcg aaactgataa cgcaaagaaa gttcgataac 
     4201 ttaactaaag ctgagagggg tggcttgtct gaacttgaca aggccggatt tattaaacgt 
     4261 cagctcgtgg aaacccgcca aatcacaaag catgttgcac agatactaga ttcccgaatg 
     4321 aatacgaaat acgacgagaa cgataagctg attcgggaag tcaaagtaat cactttaaag 
     4381 tcaaaattgg tgtcggactt cagaaaggat tttcaattct ataaagttag ggagataaat 
     4441 aactaccacc atgcgcacga cgcttatctt aatgccgtcg tagggaccgc actcattaag 
     4501 aaatacccga agctagaaag tgagtttgtg tatggtgatt acaaagttta tgacgtccgt 
     4561 aagatgatcg cgaaaagcga acaggagata ggcaaggcta cagccaaata cttcttttat 
     4621 tctaacatta tgaatttctt taagacggaa atcactctgg caaacggaga gatacgcaaa 
     4681 cgacctttaa ttgaaaccaa tggggagaca ggtgaaatcg tatgggataa gggccgggac 
     4741 ttcgcgacgg tgagaaaagt tttgtccatg ccccaagtca acatagtaaa gaaaactgag 
     4801 gtgcagaccg gagggttttc aaaggaatcg attcttccaa aaaggaatag tgataagctc 
     4861 atcgctcgta aaaaggactg ggacccgaaa aagtacggtg gcttcgatag ccctacagtt 
     4921 gcctattctg tcctagtagt ggcaaaagtt gagaagggaa aatccaagaa actgaagtca 
     4981 gtcaaagaat tattggggat aacgattatg gagcgctcgt cttttgaaaa gaaccccatc 
     5041 gacttccttg aggcgaaagg ttacaaggaa gtaaaaaagg atctcataat taaactacca 
     5101 aagtatagtc tgtttgagtt agaaaatggc cgaaaacgga tgttggctag cgccggagag 
     5161 cttcaaaagg ggaacgaact cgcactaccg tctaaatacg tgaatttcct gtatttagcg 
     5221 tcccattacg agaagttgaa aggttcacct gaagataacg aacagaagca actttttgtt 
     5281 gagcagcaca aacattatct cgacgaaatc atagagcaaa tttcggaatt cagtaagaga 
     5341 gtcatcctag ctgatgccaa tctggacaaa gtattaagcg catacaacaa gcacagggat 
     5401 aaacccatac gtgagcaggc ggaaaatatt atccatttgt ttactcttac caacctcggc 
     5461 gctccagccg cattcaagta ttttgacaca acgatagatc gcaaacgata cacttctacc 
     5521 aaggaggtgc tagacgcgac actgattcac caatccatca cgggattata tgaaactcgg 
     5581 atagatttgt cacagcttgg gggtgactga ccggtcatca tcaccatcac cattgagttt 
     5641 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 
     5701 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 
     5761 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 
     5821 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 
     5881 ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga 
     5941 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     6001 cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct 
     6061 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     6121 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     6181 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     6241 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     6301 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     6361 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     6421 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     6481 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     6541 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     6601 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     6661 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     6721 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     6781 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     6841 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     6901 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     6961 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     7021 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     7081 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     7141 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     7201 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     7261 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     7321 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     7381 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     7441 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     7501 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     7561 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     7621 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     7681 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     7741 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     7801 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     7861 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     7921 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     7981 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     8041 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     8101 tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctaggg tcgactctca 
     8161 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg 
     8221 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca 
     8281 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca 
     8341 gatatacgcg tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 
     8401 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata 
     8461 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 
     8521 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 
     8581 tgtggaaagg acgaaacacc gggtcttcga gaagacctgt tttagagcta gaaatagcaa 
     8641 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt 
     8701 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc gactagttat 
     8761 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 
     8821 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 
     8881 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 
     8941 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatc 
//
