LOCUS px330-U6-gRNA-SID4X-dCas9 8443 bp DNA circular 12-JUN-2014 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|698943477| COMMENT VNTDBDATE|700532256| COMMENT LSOWNER| COMMENT VNTNAME|px330-U6-gRNA-SID4X-dCas9| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|Dong Kong| COMMENT VNTOAUTHOREML|dkong.hms@gmail.com| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 99627112 COMMENT (CStringList) 66638152 66638328 (CObList))) FEATURES Location/Qualifiers promoter 8209..329 /vntifkey="29" /label=CMV misc_feature 8275..109 /vntifkey="21" /label=CAG-Enhancer misc_feature 6575..7505 /vntifkey="21" /label=AMP misc_feature 484..507 /vntifkey="21" /label=NLS misc_feature 412..477 /vntifkey="21" /label=3xFLAG misc_feature 964..5067 /vntifkey="21" /label=dCas9 misc_feature 541..915 /vntifkey="21" /label=SID4X misc_feature 916..963 /vntifkey="21" /label=XTEN-Linker CDS 7809..8208 /vntifkey="4" /label=px330-U6-gRNA BASE COUNT 2479 a 1867 c 2045 g 2052 t ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggactacaaa 421 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagatg 481 gcccccaaga agaagaggaa ggtgggcatt caccgcgggg tacctggagg ttctggatcc 541 atgaacatcc agatgctgct ggaggccgct gactacctgg aacggagaga gcgcgaagcc 601 gagcacggat atgcttcaat gctgcccgga agcggcatga atattcagat gctgctggag 661 gctgctgatt acctggaaag gcgcgaacgg gaggccgaac atggctatgc ttccatgctg 721 cctgggtctg gaatgaatat ccaaatgctg ctggaggcag ccgattacct ggaacggaga 781 gaaagagaag ccgagcacgg atacgccagc atgctgccag gcagcgggat gaacatacaa 841 atgctgctgg aggctgccga ttacctggag aggcgcgaga gagaagctga acatggctat 901 gcctctatgc tgcccagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa 961 agtgataaaa agtattctat tggtttagct atcggcacta attccgttgg atgggctgtc 1021 ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa cacagaccgt 1081 cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga aacggcagag 1141 gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt 1201 tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt ctttcaccgt 1261 ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc catctttgga 1321 aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca cctcagaaaa 1381 aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc tcttgcccat 1441 atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga caactcggat 1501 gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga agagaaccct 1561 ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc taaatcccga 1621 cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt gttcggtaac 1681 cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga cttagctgaa 1741 gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa tctactggca 1801 caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag cgatgcaatc 1861 ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt atccgcttca 1921 atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc cctagtccgt 1981 cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa cgggtacgca 2041 ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa acccatatta 2101 gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga tctactgcga 2161 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat 2221 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt 2281 gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct 2341 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa 2401 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag 2461 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg 2521 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta 2581 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca 2641 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc 2701 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata 2761 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg 2821 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct 2881 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga 2941 cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc 3001 gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat ccatgatgac 3061 tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg 3121 cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat actccagaca 3181 gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga aaacattgta 3241 atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg 3301 atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa ggagcatcct 3361 gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca aaatggaagg 3421 gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga cgtcgatgcc 3481 attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct tacacgctcg 3541 gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa gaaaatgaag 3601 aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt cgataactta 3661 actaaagctg agaggggtgg cttgtctgaa cttgacaagg ccggatttat taaacgtcag 3721 ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc ccgaatgaat 3781 acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac tttaaagtca 3841 aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga gataaataac 3901 taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact cattaagaaa 3961 tacccgaagc tagaaagtga gtttgtgtat ggtgattaca aagtttatga cgtccgtaag 4021 atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt cttttattct 4081 aacattatga atttctttaa gacggaaatc actctggcaa acggagagat acgcaaacga 4141 cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg ccgggacttc 4201 gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa aactgaggtg 4261 cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga taagctcatc 4321 gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc tacagttgcc 4381 tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact gaagtcagtc 4441 aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa ccccatcgac 4501 ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa actaccaaag 4561 tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc cggagagctt 4621 caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta tttagcgtcc 4681 cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact ttttgttgag 4741 cagcacaaac attatctcga cgaaatcata gagcaaattt cggaattcag taagagagtc 4801 atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca cagggataaa 4861 cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa cctcggcgct 4921 ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac ttctaccaag 4981 gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga aactcggata 5041 gatttgtcac agcttggggg tgactgaccg gtcatcatca ccatcaccat tgagtttaaa 5101 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 5161 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 5221 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 5281 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 5341 tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct 5401 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 5461 acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac 5521 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 5581 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 5641 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 5701 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 5761 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 5821 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 5881 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 5941 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 6001 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 6061 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 6181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 6241 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 6301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 6361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 6421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 6481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 6541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 6601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 6721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 6781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 6841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 6901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 6961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 7021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 7381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 7441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 7501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 7561 cacctgacgt cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta 7621 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 7681 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt 7741 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat 7801 atacgcgtga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg 7861 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt 7921 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg 7981 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt 8041 ggaaaggacg aaacaccggg tcttcgagaa gacctgtttt agagctagaa atagcaagtt 8101 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt 8161 ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgac tagttattaa 8221 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 8281 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 8341 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggac 8401 tatttacggt aaactgccca cttggcagta catcaagtgt atc //