LOCUS       pET-28a_TEE-iLOVf      5731 bp    DNA     circular UNA 2-MAY-2002
COMMENT     http://www.informaxinc.com
FEATURES             Location/Qualifiers
     Vector          complement(join(594..5731,1..230))
                     /origin="pET-28a(+) (5,368bp circular)Target vector:
                     plasmid opened by NcoI cut at 296^297Orientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
     promoter        141..157
                     /vntifkey="30"
                     /label="T7 promoter"
     CDS             160..184
                     /vntifkey="4"
                     /label="lac operator"
     primer_bind     <227..263
                     /note="Primer: tcatgaaTcacaaagtgATGATCGGTACCATTGAGAAG"
                     /note="Number of mismatches: 16"
                     /note="Number of other matches on dna20_yhaO_LOV_77: 0"
                     /note="Primer binding region: 2875..2912"
                     /label="iLOVinpET28_5p (mismatches: 16)"
     CDS             228..242
                     /label=TEE
     CDS             228..767
                     /label=TEE-iLOV-His-Thr-
     misc_feature    complement(231..593)
                     /feature_type=Insertion
                     /origin="BspHI-TEE-iLOV-NcoI (369bp linear)Fragment: from
                     BspHI cut at 1^2 to NcoI cut at 364^365,
                     363bpOrientation: reverse complementedPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label=Insert
     CDS             246..>589
                     /label=iLOV
     primer_bind     complement(567..>593)
                     /note="Primer: ccatggaATGTACGTGGTCAGAACCATC"
                     /note="Number of mismatches: 7"
                     /note="Number of other hits on dna20_yhaO_LOV_77: 0"
                     /note="Primer binding region: complement(3216..3243)"
                     /label="iLOVinpET28_3p (mismatches: 7)"
     protein_bind    603..620
                     /vntifkey="31"
                     /label="His tag"
     protein_bind    630..647
                     /vntifkey="31"
                     /label=thrombin
     protein_bind    651..683
                     /vntifkey="31"
                     /label="T7 tag"
     protein_bind    733..750
                     /vntifkey="31"
                     /label="His tag"
     terminator      818..864
                     /vntifkey="43"
                     /label="T7 terminator"
     rep_origin      900..1355
                     /vntifkey="33"
                     /label="f1 origin"
     CDS             complement(1451..2263)
                     /vntifkey="4"
                     /label="kan sequence"
     rep_origin      2972
                     /vntifkey="33"
                     /label="ColE1 pBR322 origin"
     CDS             complement(4406..5485)
                     /vntifkey="4"
                     /label="lac I"
ORIGIN
        1 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC
       61 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG
      121 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA
      181 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG AATCACAAAG
      241 TGATGATCGG TACCATTGAG AAGAATTTCG TAATCACTGA TCCACGTCTG CCGGACAACC
      301 CGATCATCTT CGCTTCCGAC GGCTTCCTGG AGCTGACCGA GTATTCCCGT GAAGAGATCC
      361 TGGGCCGTAA CGCCCGTTTC CTGCAGGGCC CGGAGACTGA TCAGGCGACG GTTCAGAAAA
      421 TTCGCGATGC TATCCGTGAC CAGCGTGAAA CTACGGTGCA ACTGATTAAC TACACGAAAA
      481 GCGGTAAGAA ATTCTGGAAC CTGCTGCATC TGCAACCAGT CCGCGATCAG AAAGGCGAGC
      541 TGCAGTATTT CATCGGCGTT CAACTGGATG GTTCTGACCA CGTACATTCC ATGGGCAGCA
      601 GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGCA
      661 TGACTGGTGG ACAGCAAATG GGTCGCGGAT CCGAATTCGA GCTCCGTCGA CAAGCTTGCG
      721 GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG
      781 GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
      841 AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATT GGCGAATGGG
      901 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
      961 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
     1021 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA
     1081 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC
     1141 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG
     1201 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT
     1261 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA
     1321 ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT
     1381 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAA
     1441 TTAATTCTTA GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT
     1501 TATCAATACC ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC
     1561 AGTTCCATAG GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA
     1621 TACAACCTAT TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG
     1681 TGACGACTGA ATCCGGTGAG AATGGCAAAA GTTTATGCAT TTCTTTCCAG ACTTGTTCAA
     1741 CAGGCCAGCC ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC
     1801 GTGATTGCGC CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG
     1861 GAATCGAATG CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT
     1921 CAGGATATTC TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG GTGAGTAACC
     1981 ATGCATCATC AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA
     2041 GCCAGTTTAG TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT
     2101 TCAGAAACAA CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT
     2161 GCCCGACATT ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA
     2221 ATCGCGGCCT AGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC
     2281 TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
     2341 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
     2401 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
     2461 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
     2521 CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
     2581 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
     2641 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
     2701 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
     2761 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
     2821 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
     2881 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
     2941 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
     3001 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
     3061 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC
     3121 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT
     3181 ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG
     3241 ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC
     3301 GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC
     3361 CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC
     3421 ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC
     3481 ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT
     3541 CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC
     3601 CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT
     3661 GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA
     3721 ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG
     3781 CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT
     3841 CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA
     3901 ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA
     3961 CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG
     4021 CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGGGCCGCCA TGCCGGCGAT
     4081 AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG
     4141 GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA
     4201 GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT
     4261 AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT
     4321 GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGAGATCCC GGTGCCTAAT GAGTGAGCTA
     4381 ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
     4441 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCCAGGG
     4501 TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC GCCTGGCCCT
     4561 GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA TCCTGTTTGA
     4621 TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT CCCACTACCG
     4681 AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG CCCAGCGCCA
     4741 TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC ATTTGCATGG
     4801 TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC GGCTGAATTT
     4861 GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG ACAGAACTTA
     4921 ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC TCCACGCCCA
     4981 GTCGCGTACC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG TCAGAGACAT
     5041 CAAGAAATAA CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA TCCTGGTCAT
     5101 CCAGCGGATA GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG TGCACCGCCG
     5161 CTTTACAGGC TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG GCACCCAGTT
     5221 GATCGGCGCG AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG GCCAGACTGG
     5281 AGGTGGCAAC GCCAATCAGC AACGACTGTT TGCCCGCCAG TTGTTGTGCC ACGCGGTTGG
     5341 GAATGTAATT CAGCTCCGCC ATCGCCGCTT CCACTTTTTC CCGCGTTTTC GCAGAAACGT
     5401 GGCTGGCCTG GTTCACCACG CGGGAAACGG TCTGATAAGA GACACCGGCA TACTCTGCGA
     5461 CATCGTATAA CGTTACTGGT TTCACATTCA CCACCCTGAA TTGACTCTCT TCCGGGCGCT
     5521 ATCATGCCAT ACCGCGAAAG GTTTTGCGCC ATTCGATGGT GTCCGGGATC TCGACGCTCT
     5581 CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC
     5641 GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG
     5701 CCTGCCACCA TACCCACGCC GAAACAAGCG C
//
LOCUS       pNIC28-Bsa4_TEE-iLOV      7645 bp    DNA     circular SYN 01-NOV-2011
DEFINITION  Expression vector pNIC28-Bsa4, complete sequence.
ACCESSION   EF198106
VERSION     EF198106.1  GI:124015065
KEYWORDS    .
SOURCE      Expression vector pNIC28-Bsa4 (unknown)
  ORGANISM  Expression vector pNIC28-Bsa4
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Stols,L., Gu,M., Dieckman,L., Raffen,R., Collart,F.R. and
            Donnelly,M.I.
  TITLE     A new vector for high-throughput, ligation-independent cloning
            encoding a tobacco etch virus protease cleavage site
  JOURNAL   Protein Expr. Purif. 25 (1), 8-15 (2002)
   PUBMED   12071693
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Keates,T., Cooper,C.D., Savitsky,P., Allerston,C.K., Phillips,C.,
            Hammarstrom,M., Daga,N., Berridge,G., Mahajan,P.,
            Burgess-Brown,N.A., Muller,S., Graslund,S. and Gileadi,O.
  TITLE     Expressing the human proteome for affinity proteomics: optimising
            expression of soluble protein domains and in vivo biotinylation
  JOURNAL   N Biotechnol (2011) In press
   PUBMED   22027370
   REMARK   Publication Status: Available-Online prior to print
REFERENCE   3  (bases 1 to 7284)
  AUTHORS   Gileadi,O., Burgess-Brown,N. and Loppnau,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-DEC-2006) Structural Genomics Consortium, University
            of Oxford, Botnar Research Centre, Oxford OX3 7LD, UK
FEATURES             Location/Qualifiers
     source          join(28..114,476..7645,1..27)
                     /organism="Expression vector pNIC28-Bsa4"
                     /mol_type="other DNA"
                     /db_xref="taxon:420517"
                     /note="derived from pET28a (Novagen)"
     Vector          join(476..7645,1..114)
                     /origin="pNIC28-Bsa4 (7,284bp circular)Target vector:
                     plasmid opened by NdeI cut at 114^115Orientation:
                     originalPlus strand 5':   no changePlus strand 3':   no
                     changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
     promoter        28..44
                     /note="T7"
     misc_feature    47..71
                     /note="lac operator"
     primer_bind     <115..152
                     /note="Primer: AttaatgaaTcacaaagtgATGATCGGTACCATTGAGAAG"
                     /note="Number of mismatches: 17"
                     /note="Number of other matches on dna20_yhaO_LOV_77: 0"
                     /note="Primer binding region: 2873..2912"
                     /label="iLOVinpNIC28_5p (mismatches: 17)"
     CDS             117..131
                     /label=TEE
     CDS             117..542
                     /label=TEE-iLOV-His-TEV-
     CDS             135..>475
                     /label=iLOV
     primer_bind     complement(456..>477)
                     /note="Primer: CATATGTACGTGGTCAGAACCATC"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on dna20_yhaO_LOV_77: 0"
                     /note="Primer binding region: complement(3216..3239)"
                     /label="iLOVinpNIC28_3p (mismatches: 0)"
     CDS             477..>542
                     /note="N-tag"
                     /codon_start=1
                     /transl_table=11
                     /product="N-terminal tag"
                     /protein_id="ABM88724.1"
                     /db_xref="GI:124015069"
                     /translation="MHHHHHHSSGVDLGTENLYFQS"
     misc_feature    480..497
                     /note="hexahistidine tag"
     misc_feature    522..542
                     /note="TEV protease cleavage site (cut after Q)"
     misc_feature    531..545
                     /note="5'-LIC sequence; add to upstream PCR primer"
     Site            join(540^541,546..551)
                     /site_type="restriction site"
                     /note="Name: BsaI"
                     /note="Pattern: ggtctc"
                     /note="cut_0_on_positive_strand: 152^153"
                     /note="cut_0_on_negative_strand: 156^157"
                     /note="inhibited_by: 5': 5-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BsaI
     Site            join(541..546,541^542)
                     /site_type="restriction site"
                     /note="Name: NcoI"
                     /note="Pattern: ccatgg"
                     /note="cut_0_on_positive_strand: 153^154"
                     /note="cut_0_on_negative_strand: 157^158"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NcoI
     gene            1004..2425
                     /gene="sacB"
     CDS             1004..2425
                     /gene="sacB"
                     /note="negative selection gene from Bacillus subtilis;
                     converts sucrose to a toxic product; allows selection of
                     recombinant plasmids on plates containing 5% sucrose"
                     /codon_start=1
                     /transl_table=11
                     /product="levansucrase"
                     /protein_id="ABM88723.1"
                     /db_xref="GI:124015068"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS
                     HITRHDMLQIPEQQKNEKYKVPEFDSSTIKNISSAKGLDVWDSWPLQNTDGTVANYHG
                     YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT
                     QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF
                     DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES
                     LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS
                     NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN
                     KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN
                     IKGKKTSVVKDSILEQGQLTVNK"
     Site            join(2465..2470,2471^2472)
                     /site_type="restriction site"
                     /note="Name: BsaI"
                     /note="Pattern: ggtctc"
                     /note="cut_0_on_positive_strand: 2083^2084"
                     /note="cut_0_on_negative_strand: 2087^2088"
                     /note="inhibited_by: 5': 5-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BsaI
     misc_feature    complement(2471..2485)
                     /note="3'-LIC sequence; add complement to downstream PCR
                     primer"
     gene            complement(3249..4064)
                     /gene="aph"
     CDS             complement(3249..4064)
                     /gene="aph"
                     /note="kanamycin resistance protein from Corynebacterium
                     diphtheriae"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="ABM88721.1"
                     /db_xref="GI:124015066"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4773
                     /note="colE1"
     gene            complement(6204..7286)
                     /gene="lacI"
     CDS             complement(6204..7286)
                     /gene="lacI"
                     /note="from Escherichia coli"
                     /codon_start=1
                     /transl_table=11
                     /product="lac repressor"
                     /protein_id="ABM88722.1"
                     /db_xref="GI:124015067"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
ORIGIN
        1 AGGATCGAGA TCTCGATCCC GCGAAATTAA TACGACTCAC TATAGGGGAA TTGTGAGCGG
       61 ATAACAATTC CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACATAATGA
      121 ATCACAAAGT GATGATCGGT ACCATTGAGA AGAATTTCGT AATCACTGAT CCACGTCTGC
      181 CGGACAACCC GATCATCTTC GCTTCCGACG GCTTCCTGGA GCTGACCGAG TATTCCCGTG
      241 AAGAGATCCT GGGCCGTAAC GCCCGTTTCC TGCAGGGCCC GGAGACTGAT CAGGCGACGG
      301 TTCAGAAAAT TCGCGATGCT ATCCGTGACC AGCGTGAAAC TACGGTGCAA CTGATTAACT
      361 ACACGAAAAG CGGTAAGAAA TTCTGGAACC TGCTGCATCT GCAACCAGTC CGCGATCAGA
      421 AAGGCGAGCT GCAGTATTTC ATCGGCGTTC AACTGGATGG TTCTGACCAC GTACATATGC
      481 ACCATCATCA TCATCATTCT TCTGGTGTAG ATCTGGGTAC CGAGAACCTG TACTTCCAAT
      541 CCATGGAGAC CGACGTCCAC ATATACCTGC CGTTCACTAT TATTTAGTGA AATGAGATAT
      601 TATGATATTT TCTGAATTGT GATTAAAAAG GCAACTTTAT GCCCATGCAA CAGAAACTAT
      661 AAAAAATACA GAGAATGAAA AGAAACAGAT AGATTTTTTA GTTCTTTAGG CCCGTAGTCT
      721 GCAAATCCTT TTATGATTTT CTATCAAACA AAAGAGGAAA ATAGACCAGT TGCAATCCAA
      781 ACGAGAGTCT AATAGAATGA GGTCGAAAAG TAAATCGCGC GGGTTTGTTA CTGATAAAGC
      841 AGGCAAGACC TAAAATGTGT AAAGGGCAAA GTGTATACTT TGGCGTCACC CCTTACATAT
      901 TTTAGGTCTT TTTTTATTGT GCGTAACTAA CTTGCCATCT TCAAACAGGA GGGCTGGAAG
      961 AAGCAGACCG CTAACACAGT ACATAAAAAA GGAGACATGA ACGATGAACA TCAAAAAGTT
     1021 TGCAAAACAA GCAACAGTAT TAACCTTTAC TACCGCACTG CTGGCAGGAG GCGCAACTCA
     1081 AGCGTTTGCG AAAGAAACGA ACCAAAAGCC ATATAAGGAA ACATACGGCA TTTCCCATAT
     1141 TACACGCCAT GATATGCTGC AAATCCCTGA ACAGCAAAAA AATGAAAAAT ATAAAGTTCC
     1201 TGAGTTCGAT TCGTCCACAA TTAAAAATAT CTCTTCTGCA AAAGGCCTGG ACGTTTGGGA
     1261 CAGCTGGCCA TTACAAAACA CTGACGGCAC TGTCGCAAAC TATCACGGCT ACCACATCGT
     1321 CTTTGCATTA GCCGGAGATC CTAAAAATGC GGATGACACA TCGATTTACA TGTTCTATCA
     1381 AAAAGTCGGC GAAACTTCTA TTGACAGCTG GAAAAACGCT GGCCGCGTCT TTAAAGACAG
     1441 CGACAAATTC GATGCAAATG ATTCTATCCT AAAAGACCAA ACACAAGAAT GGTCAGGTTC
     1501 AGCCACATTT ACATCTGACG GAAAAATCCG TTTATTCTAC ACTGATTTCT CCGGTAAACA
     1561 TTACGGCAAA CAAACACTGA CAACTGCACA AGTTAACGTA TCAGCATCAG ACAGCTCTTT
     1621 GAACATCAAC GGTGTAGAGG ATTATAAATC AATCTTTGAC GGTGACGGAA AAACGTATCA
     1681 AAATGTACAG CAGTTCATCG ATGAAGGCAA CTACAGCTCA GGCGACAACC ATACGCTGAG
     1741 AGATCCTCAC TACGTAGAAG ATAAAGGCCA CAAATACTTA GTATTTGAAG CAAACACTGG
     1801 AACTGAAGAT GGCTACCAAG GCGAAGAATC TTTATTTAAC AAAGCATACT ATGGCAAAAG
     1861 CACATCATTC TTCCGTCAAG AAAGTCAAAA ACTTCTGCAA AGCGATAAAA AACGCACGGC
     1921 TGAGTTAGCA AACGGCGCTC TCGGTATGAT TGAGCTAAAC GATGATTACA CACTGAAAAA
     1981 AGTGATGAAA CCGCTGATTG CATCTAACAC AGTAACAGAT GAAATTGAAC GCGCGAACGT
     2041 CTTTAAAATG AACGGCAAAT GGTACCTGTT CACTGACTCC CGCGGATCAA AAATGACGAT
     2101 TGACGGCATT ACGTCTAACG ATATTTACAT GCTTGGTTAT GTTTCTAATT CTTTAACTGG
     2161 CCCATACAAG CCGCTGAACA AAACTGGCCT TGTGTTAAAA ATGGATCTTG ATCCTAACGA
     2221 TGTAACCTTT ACTTACTCAC ACTTCGCTGT ACCTCAAGCG AAAGGAAACA ATGTCGTGAT
     2281 TACAAGCTAT ATGACAAACA GAGGATTCTA CGCAGACAAA CAATCAACGT TTGCGCCTAG
     2341 CTTCCTGCTG AACATCAAAG GCAAGAAAAC ATCTGTTGTC AAAGACAGCA TCCTTGAACA
     2401 AGGACAATTA ACAGTTAACA AATAAAAACG CAAAAGAAAA TGCCGATATC CTATTGGCAT
     2461 TGACGGTCTC CAGTAAAGGT GGATACGGAT CCGAATTCGA GCTCCGTCGA CAAGCTTGCG
     2521 GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG
     2581 GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     2641 AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATT GGCGAATGGG
     2701 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
     2761 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
     2821 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA
     2881 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC
     2941 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG
     3001 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT
     3061 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA
     3121 ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCAGG TGGCACTTTT CGGGGAAATG
     3181 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
     3241 ATTAATTCTT AGAAAAACTC ATCGAGCATC AAATGAAACT GCAATTTATT CATATCAGGA
     3301 TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG AAGGAGAAAA CTCACCGAGG
     3361 CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA TTCCGACTCG TCCAACATCA
     3421 ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT CAAGTGAGAA ATCACCATGA
     3481 GTGACGACTG AATCCGGTGA GAATGGCAAA AGTTTATGCA TTTCTTTCCA GACTTGTTCA
     3541 ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT CAACCAAACC GTTATTCATT
     3601 CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT TAAAAGGACA ATTACAAACA
     3661 GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT CAACAATATT TTCACCTGAA
     3721 TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCGG GGATCGCAGT GGTGAGTAAC
     3781 CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG GAAGAGGCAT AAATTCCGTC
     3841 AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACATCATTGG CAACGCTACC TTTGCCATGT
     3901 TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC GATAGATTGT CGCACCTGAT
     3961 TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT
     4021 AATCGCGGCC TAGAGCAAGA CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA
     4081 CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
     4141 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
     4201 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
     4261 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
     4321 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
     4381 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
     4441 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
     4501 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
     4561 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
     4621 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
     4681 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
     4741 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
     4801 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
     4861 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC
     4921 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT
     4981 TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT
     5041 GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG
     5101 CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT
     5161 CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT
     5221 CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT
     5281 CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG
     5341 TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG
     5401 CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA
     5461 TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA
     5521 AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC
     5581 GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA
     5641 TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA
     5701 AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC
     5761 ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA
     5821 GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGGGCCGCC ATGCCGGCGA
     5881 TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA
     5941 GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA
     6001 AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA
     6061 TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC
     6121 TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGAGATCC CGGTGCCTAA TGAGTGAGCT
     6181 AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC
     6241 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG
     6301 GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC
     6361 TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG
     6421 ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC
     6481 GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC
     6541 ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG
     6601 GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT
     6661 TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT
     6721 AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC
     6781 AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA
     6841 TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA
     6901 TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC
     6961 GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT
     7021 TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG
     7081 GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC CACGCGGTTG
     7141 GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT CGCAGAAACG
     7201 TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG AGACACCGGC ATACTCTGCG
     7261 ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTCTC TTCCGGGCGC
     7321 TATCATGCCA TACCGCGAAA GGTTTTGCGC CATTCGATGG TGTCCGGGAT CTCGACGCTC
     7381 TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC
     7441 CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG
     7501 GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC
     7561 TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT
     7621 GCCGGCCACG ATGCGTCCGG CGTAG
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