LOCUS       pSUC2-GFPantisen        7177 bp    DNA     circular UNA 04-MAR-2015
DEFINITION  Digestion of yEGFP3-CLN2PEST CDS (reversed) - Fragment 2 inserted
            into pSUC2-CYC1t-413.
ACCESSION   urn.local...ba-46ipvpv
VERSION     urn.local...ba-46ipvpv
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             504..1160
                     /gene="HIS3"
     misc_feature    1555..1861
                     /gene="fi ori"
     primer_bind     2023..2039
                     /Hairpin="4.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="187788797612700ea393d95-9d77-480a-9ec2-
                     d898e4b80f5f"
                     /%GC="52.9"
                     /Tm="54.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GTAAAACGACGGCCAGT"
                     /label="M13_forward20_primer"
     misc_feature    2049..2067
                     /gene="T7 promoter"
     misc_feature    order(<4386..4401,<4061..>4082,<2790..>2810,2068..>2086)
                     /gene="MCS"
     misc_feature    <2083..2087
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        2083..2086
                     /label="Ligation"
     misc_feature    <2083..>2087
                     /gene="MCS"
     promoter        2087..2789
                     /Source="referenceToDocument: urn:local:.:57-3k520fp"
                     /modified_by="User"
                     /label="SUC2 promoter"
     misc_feature    2788..>2791
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    <2788..>2791
                     /gene="MCS"
     ligation        2790..2791
                     /label="Ligation"
     misc_feature    <2807..2811
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        2807..2810
                     /label="Ligation"
     misc_RNA        2811..4060
                     /Source="referenceToDocument: urn:local:.:av-46ipura"
                     /modified_by="User"
                     /label="yEGFP3-CLN2PEST antisense"
     primer_bind     2812..2833
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="152452710252645486bd92-1bf5-4c75-ad2e-e
                     b91a1d80d46"
                     /%GC="40.9"
                     /Tm="53.8"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="CTATATTACTTGGGTATTGCCC"
                     /label="GFP1F"
     primer_bind     2861..2884
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1523044064594631e67648-e9b3-45cd-a759-d
                     96905393f08"
                     /%GC="37.5"
                     /Tm="55.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="CTGCTAGATTTACCGCTATTTATG"
                     /label="GFP2F"
     primer_bind     2911..2932
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15148197703984e4d8dfcf-3778-47a8-8b51-6
                     a2a55871c5f"
                     /%GC="36.4"
                     /Tm="55.0"
                     /Hairpin_Tm="34.6"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="ATGGTTAATGAAGCTTGTCTTG"
                     /label="GFP3F"
     primer_bind     2961..2980
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15138694466490a41e15a4-913e-41b7-a8ce-d
                     600f4b0a1d6"
                     /%GC="45.0"
                     /Tm="57.5"
                     /Hairpin_Tm="41.1"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTGCGGGATTTTGGTTTTCC"
                     /label="GFP4F"
     primer_bind     3011..3032
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1512755079804955a24cc8-0e4e-411f-9be3-e
                     b8f5df3bb9e"
                     /%GC="27.3"
                     /Tm="51.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="ATATAATCATTGGTTGCGTTAT"
                     /label="GFP5F"
     primer_bind     3061..3084
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15114312560911019b8b15-f051-4723-a7a7-2
                     b758fb77b13"
                     /%GC="25.0"
                     /Tm="52.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GATCCTTTGTTTGTTATTTGTATT"
                     /label="GFP6F"
     primer_bind     3111..3132
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="150951760943869435fffd-1ff7-4cc7-8f9a-4
                     2d868afed6a"
                     /%GC="36.4"
                     /Tm="53.3"
                     /Hairpin_Tm="35.3"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TACCACATATACTGTTTGACTG"
                     /label="GFP7F"
     primer_bind     3161..3181
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="150841482170362d4f5e03-16e6-41ac-9d28-7
                     b1025abf332"
                     /%GC="42.9"
                     /Tm="55.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GTGATTGGTGATGATTGTTCC"
                     /label="GFP8F"
     primer_bind     3211..3232
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15073259569245df9091a2-51dd-477d-8c35-0
                     aa6658c8f91"
                     /%GC="40.9"
                     /Tm="56.4"
                     /Hairpin_Tm="41.3"
                     /Self_Dimer_Tm="20.6"
                     /Pair_Dimer_Tm="17.4"
                     /Sequence="GAGTGAGCTCATGTTTCTCATT"
                     /label="GFP9F"
     primer_bind     3261..3280
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="150627423869819324223a-7d60-4672-99d4-8
                     e6fee3eb656"
                     /%GC="50.0"
                     /Tm="58.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GCGAAGGAATGGATGTGCTA"
                     /label="GFP10F"
     primer_bind     3311..3332
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15049665385827e5b2a835-b886-47e2-986e-8
                     b98c87d2ae2"
                     /%GC="36.4"
                     /Tm="54.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.4"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GATATGGTAAGCTTTCTCGAAA"
                     /label="GFP11F"
     primer_bind     3361..3381
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14731284736814cbad3bbc-96ca-4007-b503-c
                     784ff876411"
                     /%GC="42.9"
                     /Tm="54.2"
                     /Hairpin_Tm="50.4"
                     /Self_Dimer_Tm="5.4"
                     /Pair_Dimer_Tm="10.6"
                     /Sequence="ATCCATACCATGGGTAATACC"
                     /label="GFP12F"
     primer_bind     3411..3431
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15011161889449bcb62a84-a45e-4802-830e-c
                     a3d77478edf"
                     /%GC="42.9"
                     /Tm="56.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TGTGGTCTCTCTTTTCGTTTG"
                     /label="GFP13F"
     misc_feature    complement(3439..3460)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15011161889449bcb62a84-a45e-4802-830e-c
                     a3d77478edf"
                     /%GC="45.5"
                     /Tm="57.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="ATCCACTCAATCTGCCTTATCC"
                     /label="GFP13R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3491..3510)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14731284736814cbad3bbc-96ca-4007-b503-c
                     784ff876411"
                     /%GC="40.0"
                     /Tm="52.9"
                     /Hairpin_Tm="36.3"
                     /Self_Dimer_Tm="19.3"
                     /Pair_Dimer_Tm="10.6"
                     /Sequence="AATACTCCAATTGGTGATGG"
                     /label="GFP12R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3541..3560)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15049665385827e5b2a835-b886-47e2-986e-8
                     b98c87d2ae2"
                     /%GC="40.0"
                     /Tm="53.8"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GACACAACATTGAAGATGGT"
                     /label="GFP11R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3589..3610)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="150627423869819324223a-7d60-4672-99d4-8
                     e6fee3eb656"
                     /%GC="40.9"
                     /Tm="56.8"
                     /Hairpin_Tm="33.2"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="CATCATGGCTGACAAACAAAAG"
                     /label="GFP10R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3639..3660)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15073259569245df9091a2-51dd-477d-8c35-0
                     aa6658c8f91"
                     /%GC="31.8"
                     /Tm="53.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="17.4"
                     /Sequence="AACATTTTAGGTCACAAATTGG"
                     /label="GFP9R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3684..3710)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="150841482170362d4f5e03-16e6-41ac-9d28-7
                     b1025abf332"
                     /%GC="25.9"
                     /Tm="53.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="7.7"
                     /Pair_Dimer_Tm="None"
                     /Sequence="CCTTAGTTAATAGAATCGAATTAAAAG"
                     /label="GFP8R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3741..3760)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="150951760943869435fffd-1ff7-4cc7-8f9a-4
                     2d868afed6a"
                     /%GC="40.0"
                     /Tm="53.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="AGATGACGGTAACTACAAGA"
                     /label="GFP7R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3792..3810)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15114312560911019b8b15-f051-4723-a7a7-2
                     b758fb77b13"
                     /%GC="52.6"
                     /Tm="57.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="AAGTCTGCCATGCCAGAAG"
                     /label="GFP6R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3840..3860)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1512755079804955a24cc8-0e4e-411f-9be3-e
                     b8f5df3bb9e"
                     /%GC="38.1"
                     /Tm="54.9"
                     /Hairpin_Tm="32.6"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTCAATGTTTTGCGAGATACC"
                     /label="GFP5R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3892..3910)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15138694466490a41e15a4-913e-41b7-a8ce-d
                     600f4b0a1d6"
                     /%GC="42.1"
                     /Tm="53.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.0"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TAAATTGCCAGTTCCATGG"
                     /label="GFP4R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3941..3960)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="15148197703984e4d8dfcf-3778-47a8-8b51-6
                     a2a55871c5f"
                     /%GC="45.0"
                     /Tm="54.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GGTGATGCTACTTACGGTAA"
                     /label="GFP3R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3990..4010)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1523044064594631e67648-e9b3-45cd-a759-d
                     96905393f08"
                     /%GC="38.1"
                     /Tm="52.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TAGATGGTGATGTTAATGGTC"
                     /label="GFP2R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4037..4059)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="152452710252645486bd92-1bf5-4c75-ad2e-e
                     b91a1d80d46"
                     /%GC="30.4"
                     /Tm="52.2"
                     /Hairpin_Tm="52.0"
                     /Self_Dimer_Tm="29.4"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TCTAAAGGTGAAGAATTATTCAC"
                     /label="GFP1R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    4060..>4064
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        4061..4064
                     /label="Ligation"
     misc_feature    <4079..4083
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        4079..4082
                     /label="Ligation"
     terminator      4083..4385
                     /Source="referenceToDocument:
                     urn:local:.:1346893798854.130"
                     /modified_by="User"
                     /label="CYC1t terminator"
     misc_feature    complement(4364..4383)
                     /created_by="primer3"
                     /%GC="45.0"
                     /Tm="49.85"
                     /Hairpin="6.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="ACGATGAGAGTGTAAACTGC"
                     /Mismatches=0
                     /Pair_Hairpin="3.0"
                     /Pair_Primer-Dimer="0.0"
                     /Pair_Tm_Diff="3.87"
                     /Product_Size="302.0"
                     /annotation_group="PrimerPlugin
                     Group5e9c3d6f-ce6c-4414-bbdb-fd24df2569aa_urn:local:.:1339
                     649544705.8"
                     /label="CYC1GR"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    4385..>4389
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        4386..4389
                     /label="Ligation"
     misc_feature    complement(4402..4421)
                     /gene="T3 promoter"
     misc_feature    complement(4438..4456)
                     /Hairpin="4.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /Pair_Hairpin="3.0"
                     /Pair_Primer-Dimer="1.0"
                     /Pair_Tm_Diff="1.39"
                     /Product_Size="227.0"
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="187788797612700ea393d95-9d77-480a-9ec2-
                     d898e4b80f5f"
                     /%GC="47.4"
                     /Tm="53.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GGAAACAGCTATGACCATG"
                     /label="M13_reverse_primer"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    4829..5448
                     /gene="pBR322 ori"
     misc_feature    5603..6463
                     /gene="AmpR"
     misc_feature    6505..6533
                     /gene="AmpR promoter"
     misc_feature    6595..7113
                     /gene="CEN6/ARS4"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccaca tactaagaca
     2101 tttaccgtat gggagttgtt gtcctagcgt agttctcgct cccccagcaa agctcaaaaa
     2161 agtacgtcat ttagaatagt ttgtgagcaa attaccagtc ggtatgctac gttagaaagg
     2221 cccacagtat tcttctacca aaggcgtgcc tttgttgaac tcgatccatt atgagggctt
     2281 ccattattcc ccgcattttt attactctga acaggaataa aaagaaaaaa cccagtttag
     2341 gaaattatcc gggggcgaag aaatacgcgt agcgttaatc gaccccacgt ccagggtttt
     2401 tccatggagg tttctggaaa aactgacgag gaatgtgatt ataaatccct ttatgtgatg
     2461 tctaagactt ttaaggtacg cccgatgttt gcctattacc atcatagaga cgtttctttt
     2521 cgaggaatgc ttaaacgact ttgtttgaca aaaatgttgc ctaagggctc tatagtaaac
     2581 catttggaag aaagatttga cgactttttt tttttggatt tcgatcctat aatccttcct
     2641 cctgaaaaga aacatataaa tagatatgta ttattcttca aaacattctc ttgttcttgt
     2701 gctttttttt taccatatat cttacttttt tttttctctc agagaaacaa gcaaaacaaa
     2761 aagcttttct tttcactaac gtatatgatc gataagcttg atatcgaatt cctatattac
     2821 ttgggtattg cccataccaa aagaaattaa agatgaggca ctgctagatt taccgctatt
     2881 tatggtccca gttggcgagg ggaacattcc atggttaatg aagcttgtct tgggtgggcc
     2941 cccattgagg taatgcgccg ttgcgggatt ttggttttcc ttgttagact catttaggtt
     3001 tagcaaatca atataatcat tggttgcgtt attgctgtta ggacccgtga tttgttcgta
     3061 gatcctttgt ttgttatttg tattcaccag actattcaca ctaacggtgc taccacatat
     3121 actgtttgac tgctgctgac caaattggta catacttgga gtgattggtg atgattgttc
     3181 catattccgg ctgaaaacgc tgttatcaga gagtgagctc atgtttctca ttggagtgtg
     3241 gctttgagat gaggaagcgg gcgaaggaat ggatgtgcta tttgaatttt cgaaagagca
     3301 tgatggggtt gatatggtaa gctttctcga aatgttcaag ttggatgctt tgtacaattc
     3361 atccatacca tgggtaatac cagcagcagt aacaaattct aacaagacca tgtggtctct
     3421 cttttcgttt ggatctttgg ataaggcaga ttgagtggat aagtaatggt tgtctggtaa
     3481 caagactgga ccatcaccaa ttggagtatt ttgttgataa tggtcagcta attgaacaga
     3541 accatcttca atgttgtgtc taattttgaa gttaactttg ataccattct tttgtttgtc
     3601 agccatgatg taaacattgt gagagttata gttgtattcc aatttgtgac ctaaaatgtt
     3661 accatcttct ttaaaatcaa taccttttaa ttcgattcta ttaactaagg tatcaccttc
     3721 aaacttgact tcagctctgg tcttgtagtt accgtcatct ttgaaaaaaa tagttctttc
     3781 ttgaacataa ccttctggca tggcagactt gaaaaagtca tgttgtttca tatgatctgg
     3841 gtatctcgca aaacattgaa caccataacc gaaagtagtg actaaggttg gccatggaac
     3901 tggcaattta ccagtagtac aaataaattt taaggtcaat ttaccgtaag tagcatcacc
     3961 ttcaccttca ccggagacag aaaatttgtg accattaaca tcaccatcta attcaaccaa
     4021 aattgggaca acaccagtga ataattcttc acctttagac ccgggggatc cactagttct
     4081 agaaggcccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct tacattcacg
     4141 ccctcctccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     4201 ccctatttat tttttttaat agttatgtta gtattaagaa cgttatttat atttcaaatt
     4261 tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
     4321 gagaaggttt tgggacgctc gaaggcttta atttgcaagc ttcgcagttt acactctcat
     4381 cgtcgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     4441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag
     4501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg
     4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5281 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcct
     6601 tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt
     6661 aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta
     6721 aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag
     6781 gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg
     6841 attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct
     6901 aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt
     6961 ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa
     7021 acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga
     7081 ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa ccattattat catgacatta
     7141 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
