LOCUS       pLJSRSF7_(MBP_Sm        5653 bp    DNA     circular UNA 07-AUG-2013
DEFINITION  RSF vector with His-MBP-Smt3.
ACCESSION   urn.local...1369663700983.38
VERSION     urn.local...1369663700983.38
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /note="Geneious type: promoter prokaryotic"
                     /label="T7 Promoter"
     misc_feature    22..42
                     /note="Geneious type: protein_binding"
                     /label="lacO"
     RBS             75..80
                     /label="RBS-1"
     misc_feature    100..117
                     /note="Geneious type: motif"
                     /label="6xHis"
     misc_feature    118..1233
                     /note="Geneious type: Editing History Replacement"
                     /label="AGC"
     misc_feature    118..1234
                     /note="Geneious type: motif"
                     /label="MBP"
     CDS             1237..1527
                     /label="Smt3"
     CDS             1708..2014
                     /label="NpuDnaE-N102"
     misc_feature    2026..2178
                     /label="CBD"
     misc_feature    2207..2251
                     /note="Geneious type: protein_binding"
                     /label="S-Tag"
     misc_feature    complement(2289..2307)
                     /note="Geneious type: primer"
                     /label="T7 Terminator primer"
     terminator      2303..2350
                     /label="T7 Terminator"
     CDS             complement(2580..3395)
                     /label="Kan"
     RBS             complement(3401..3405)
                     /label="RBS"
     -10_signal      complement(3437..3443)
                     /label="-10 Ap"
     -35_signal      complement(3460..3465)
                     /label="-35 Ap"
     rep_origin      3503..4252
                     /label="RSF ori - RSF1030 Homology"
     misc_RNA        complement(3503..4201)
                     /label="RNA II"
     -35_signal      4056..4061
                     /label="-35 RNA I"
     -10_signal      4080..4085
                     /label="-10 RNA I"
     misc_RNA        4092..4198
                     /label="RNA I"
     CDS             complement(4448..5530)
                     /label="lacI"
ORIGIN      
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taataaggag atataccatg ggcagcagcc atcatcatca tcatcacggc
      121 aaaatcgaag aaggtaaacg taaactggta atctggatta acggcgataa aggctataac
      181 ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag
      241 catccggata aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac
      301 attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa
      361 atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt
      421 tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac
      481 aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa
      541 ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg
      601 ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt
      661 aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg
      721 attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat
      781 aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc
      841 aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc
      901 gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcaaaagag
      961 ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa agacaaaccg
     1021 ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc acgtattgcc
     1081 gccactatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca gatgtccgct
     1141 ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat
     1201 gaagccctga aagacgcgca gactaattcg ggtactagtg actcagaagt caatcaagaa
     1261 gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt aaaggtgtcc
     1321 gatggatctt cagaaatctt ctttaagatc aaaaagacca ctcctttaag aaggctgatg
     1381 gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt gtacgacggt
     1441 attagaattc aagctgatca gacccctgaa gatttggaca tggaggataa cgatattatt
     1501 gagactcaca gagaacagat tggtggatcc catatgcagt acaaacttat cctgaacggt
     1561 aaaaccctga aaggtgaaac caccaccgaa gctgttgacg ctgctaccgc ggaaaaagtt
     1621 ttcaaacagt acgctaacga caacggtgtt gacggtgaat ggacctacga cgacgctacc
     1681 aaaacctaca ccgtaacgga aggatcctgt ttaagctatg aaacggaaat attgacagta
     1741 gaatatggat tattaccgat tggtaaaatt gtagaaaagc gcatcgaatg tactgtttat
     1801 agcgttgata ataatggaaa tatttataca caacctgtag cacaatggca cgatcgcgga
     1861 gaacaagagg tgtttgagta ttgtttggaa gatggttcat tgattcgggc aacaaaagac
     1921 cataagttta tgactgttga tggtcaaatg ttgccaattg atgaaatatt tgaacgtgaa
     1981 ttggatttga tgcgggttga taatttgccg aacgaattcg gtaccacaaa tcctggtgta
     2041 tccgcttggc aggtcaacac agcttatact gcgggacaat tggtcacata taacggcaag
     2101 acgtataaat gtttgcagcc ccacacctcc ttggcaggat gggaaccatc caacgttcct
     2161 gccttgtggc agcttcaata agcttgcggc cgcactcgag tctggtaaag aaaccgctgc
     2221 tgcgaaattt gaacgccagc acatggactc gtctactagc gcagcttaat taacctaggc
     2281 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     2341 gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt
     2401 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
     2461 gacgacaagc tgacgaccgg gtctccgcaa gtggcacttt tcggggaaat gtgcgcggaa
     2521 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct
     2581 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     2641 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     2701 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     2761 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     2821 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     2881 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     2941 gcctgagcga gacgaaatac gcggtcgctg ttaaaaggac aattacaaac aggaatcgaa
     3001 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     3061 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     3121 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     3181 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     3241 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     3301 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     3361 ctagagcaag acgtttcccg ttgaatatgg ctcatactct tcctttttca atattattga
     3421 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3481 aaacaaatag gcatgcagcg ctcttccgct tcctcgctca ctgactcgct acgctcggtc
     3541 gttcgactgc ggcgagcggt gtcagctcac tcaaaagcgg taatacggtt atccacagaa
     3601 tcaggggata aagccggaaa gaacatgtga gcaaaaagca aagcaccgga agaagccaac
     3661 gccgcaggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3721 aagccagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt tggtatctca gttcggtgta
     3901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4021 agcagccatt ggtaactgat ttagaggact ttgtcttgaa gttatgcacc tgttaaggct
     4081 aaactgaaag aacagatttt ggtgagtgcg gtcctccaac ccacttacct tggttcaaag
     4141 agttggtagc tcagcgaacc ttgagaaaac caccgttggt agcggtggtt tttctttatt
     4201 tatgagatga tgaatcaatc ggtctatcaa gtcaacgaac agctattccg ttactctaga
     4261 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     4321 tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     4381 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     4441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     4561 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     4621 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     4681 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagatg tccgcaccaa
     4741 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     4801 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     4861 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     4921 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     4981 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     5041 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     5101 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     5161 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     5221 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     5281 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     5341 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     5401 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     5461 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     5521 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     5581 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     5641 tgcattagga aat
//
