LOCUS pFGET19_Ulp1 5979 bp DNA circular 14-OCT-2013 DEFINITION Ulp1 from pHKRS52 into pET-28b(+). ACCESSION urn.local...1381394133664.9 VERSION KEYWORDS . SOURCE null ORGANISM . FEATURES Location/Qualifiers misc_feature 10..15 /Recognition_pattern="A^GATCT" /annotation_group="AGATCT" /label="BglII" /note="Geneious type: restriction site" misc_feature 30..46 /label="T7 promoter" /note="Geneious type: promoter prokaryotic" primer 30..49 /gene="Promoter" /note="PROMOTER T7 RNA polymerase promoter [Cloning vector PinPoint Xa-1, complete sequence (CVU47626)]" /NOTE="T7 RNA polymerase promoter"" complement(938..956)" /gene="T7 rev" /note="primer [pHYRSF53(Sumo)LA]" /label="T7 Terminator primer" prot_bind 49..73 /gene="Protein binding" /note="PROTEIN_BIND" /modified_by="User" /label="lac operator" protein_binding 51..71 /label="lacO" misc_feature 76..81 /Recognition_pattern="T^CTAGA" /annotation_group="TCTAGA" /label="XbaI" /note="Geneious type: restriction site" misc_feature 115..120 /Recognition_pattern="CA^TATG" /annotation_group="CATATG" /label="NdeI" /note="Geneious type: restriction site" misc_feature 116..147 /Original_Bases="CATGGGCAGCAGCCATCATCATCATCATCAC" /label="CATGGGCAGCAGCCATCATCATCATCATCAC" /note="Geneious type: Editing History Replacement" CDS <118..828 /gene="His-Ulp1" /note="Unknown open reading frame [fragm Ulp1]" /codon_start=1 /modified_by="User" /label="His-Ulp1 CDS" misc_feature 163..168 /Recognition_pattern="G^GATCC" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 212..217 /Recognition_pattern="A^AGCTT" /annotation_group="AAGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 223..228 /Recognition_pattern="T^CTAGA" /annotation_group="TCTAGA" /label="XbaI" /note="Geneious type: restriction site" misc_feature 829..834 /Recognition_pattern="G^AATTC" /annotation_group="GAATTC" /label="EcoRI" /note="Geneious type: restriction site" misc_feature 842..847 /Recognition_pattern="G^TCGAC" /annotation_group="GTCGAC" /label="SalI" /note="Geneious type: restriction site" misc_feature 848..853 /Recognition_pattern="A^AGCTT" /annotation_group="AAGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 854..861 /Recognition_pattern="GC^GGCCGC" /annotation_group="GCGGCCGC" /label="NotI" /note="Geneious type: restriction site" misc_feature 863..868 /Recognition_pattern="C^TCGAG" /annotation_group="CTCGAG" /label="XhoI" /note="Geneious type: restriction site" primer complement(939..957) /label="T7 Terminator primer" terminator 953..1000 /gene="Terminator" /note="TERMINATOR [pHYRSF53(Sumo)LA]" /label="T7 Terminator" rep_origin 1037..1492 /gene="Origin of replication" /note="REP_ORIGIN" /label="f1 origin" CDS complement(1588..2400) /gene="CDS" /note="CDS" /label="kan sequence" misc_feature 1852..1857 /Recognition_pattern="ATGCA^T" /annotation_group="ATGCAT" /label="NsiI" /note="Geneious type: restriction site" misc_feature 2118..2123 /Recognition_pattern="ATGCA^T" /annotation_group="ATGCAT" /label="NsiI" /note="Geneious type: restriction site" rep_origin 2522..3110 /gene="ColE ori" /note="[pAc-G2T]" /label="ColE ori rep origin" CDS complement(4543..5622) /gene="CDS" /note="CDS" /label="lac I" misc_feature 4819..4824 /Recognition_pattern="GAT^ATC" /annotation_group="GATATC" /label="EcoRV" /note="Geneious type: restriction site" misc_feature 5267..5272 /Recognition_pattern="A^CGCGT" /annotation_group="ACGCGT" /label="MluI" /note="Geneious type: restriction site" ORIGIN 1 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc 61 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg 121 cggggttctc atcatcatca tcatcatggt ctggttccgc gtggatccct tgttcctgaa 181 ttaaatgaaa aagacgatga ccaagtacaa aaagctttgg catctagaga aaatactcag 241 ttaatgaata gagataatat agagataaca gtacgtgatt ttaagacctt ggcaccacga 301 agatggctaa atgacactat cattgagttt tttatgaaat acattgaaaa atctacccct 361 aatacagtgg cgtttaattc gtttttctat accaatttat cagaaagggg ttatcaaggc 421 gtccggaggt ggatgaagag aaagaagaca caaattgata aacttgataa aatctttaca 481 ccaataaatt tgaaccaatc ccactgggcg ttgggcataa ttgatttaaa aaagaaaact 541 ataggttacg tagattcatt atcgaatggt ccaaatgcta tgagtttcgc tatactgact 601 gacttgcaaa aatatgttat ggaggaaagt aagcatacaa taggagaaga ctttgatttg 661 attcatttag attgtccgca gcaaccaaat ggctacgact gtggaatata tgtttgtatg 721 aatactctct atggaagtgc agatgcgcca ttggattttg attataaaga tgcgattagg 781 atgagaagat ttattgccca tttgatttta accgacgctt taaaataaga attcgagctc 841 cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc 901 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata 961 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc 1021 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 1081 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 1141 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 1201 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 1261 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 1321 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 1381 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 1441 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc 1501 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 1561 atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa 1621 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg 1681 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc 1741 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag 1801 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc 1861 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac 1921 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa 1981 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac 2041 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat 2101 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag 2161 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac 2221 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata 2281 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc 2341 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat 2401 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc 2461 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 2521 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 2581 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 2641 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 2701 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 2761 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 2821 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 2881 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 2941 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 3001 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 3061 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 3121 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 3181 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 3241 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga 3301 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct 3361 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt 3421 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 3481 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 3541 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg 3601 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc 3661 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc 3721 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg 3781 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg 3841 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact 3901 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag 3961 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga 4021 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg 4081 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg 4141 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg 4201 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg 4261 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc 4321 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt 4381 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc 4441 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt 4501 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg 4561 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 4621 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc 4681 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag 4741 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc 4801 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg 4861 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc 4921 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc 4981 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg 5041 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac 5101 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg 5161 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc 5221 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag 5281 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac 5341 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc 5401 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg 5461 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg 5521 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac 5581 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg 5641 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc 5701 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt 5761 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca 5821 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga 5881 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac 5941 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgt //