LOCUS       pc'CaNAR(Cer3-YPet).xdna    7857 bp      DNA     circular   7/8/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     gene            2632..3345
                     /label=YPET
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1009..1725
                     /label=CER3
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1732..2630
                     /label=NFAT 1-297
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5696..5716)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3410..3427)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(6084..6712)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5722..5744)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6864..7523)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4565..5356
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7763..7791)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5749..5778)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4346..4423
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3450..3677
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCcAT GGTGAGCAAG 
     1021 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 
     1081 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 
     1141 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 
     1201 CTGAGCTGGG GCGTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 
     1261 TTCAAGTCCG CCATGCCCGA AGGCTACgTC cAGGAgCGCA CCATCTTCTT CAAGGACGAC 
     1321 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 
     1381 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 
     1441 AACGCCATCC ACGGCAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC 
     1501 AACTTCGGCC TCAACTGCAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG 
     1561 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC 
     1621 CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 
     1681 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGcgcat gcccatgaac 
     1741 gcccccgagc ggcagcccca acccgacggc ggggacgccc caggccacga gcctgggggc 
     1801 agcccccaag acgagcttga cttctccatc ctcttcgact atgagtattt gaatccgaac 
     1861 gaagaagagc cgaatgcaca taaggtcgcc agcccaccct ccggacccgc ataccccgat 
     1921 gatgtaatgg actatggcct caagccatac agcccccttg ctagtctctc tggcgagccc 
     1981 cccggccgat tcggagagcc ggatagggta gggccgcaga agtttctgag cgcggccaag 
     2041 ccagcagggg cctcgggcct gagccctcgg atcgagatca ctccgtccca cgaactgatc 
     2101 caggcagtgg ggcccctccg catgagagac gcgggcctcc tggtggagca gcctcccctg 
     2161 gccggggtgg ccgccagccc gaggttcacc ctgcccgtgc ccggcttcga gggctaccgc 
     2221 gagccgcttt gcttgagccc cgctagcagc ggctcctctg ccagcttcat ttctgacacc 
     2281 ttctccccct acacctcgcc ctgcgtctcg cccaataacg gcgggcccga cgacctgtgt 
     2341 ccgcagtttc aaaacatccc tgctcattat tcccccagaa cctcgccaat aatgtcacct 
     2401 cgaaccagcc tcgccgagga cagctgcctg ggccgccact cgcccgtgcc ccgtccggcc 
     2461 tcccgctcct catcgcctgg tgccaagcgg aggcattcgt gcgccgaggc cttggttgcc 
     2521 ctgccgcccg gagcctcacc ccagcgctcc cggagcccct cgccgcagcc ctcatctcac 
     2581 gtggcacccc aggaccacgg ctccccggct gggtaccccc ctgttgagct cTCTAAAGGT 
     2641 GAAGAATTAT TCACTGGTGT TGTCCCAATT TTGGTTGAAT TAGATGGTGA TGTTAATGGT 
     2701 CACAAATTTT CTGTCTCCGG TGAAGGTGAA GGTGATGCTA CGTACGGTAA ATTGACCTTA 
     2761 AAATTACTCT GTACTACTGG TAAATTGCCA GTTCCATGGC CAACCTTAGT CACTACTTTA 
     2821 GGTTATGGTG TTCAATGTTT TGCTAGATAC CCAGATCATA TGAAACAACA TGACTTTTTC 
     2881 AAGTCTGCCA TGCCAGAAGG TTATGTTCAA GAAAGAACTA TTTTTTTCAA AGATGACGGT 
     2941 AACTACAAGA CCAGAGCTGA AGTCAAGTTT GAAGGTGATA CCTTAGTTAA TAGAATCGAA 
     3001 TTAAAAGGTA TTGATTTTAA AGAAGATGGT AACATTTTAG GTCACAAATT GGAATACAAC 
     3061 TATAACTCTC ACAATGTTTA CATCACTGCT GACAAACAAA AGAATGGTAT CAAAGCTAAC 
     3121 TTCAAAATTA GACACAACAT TGAAGATGGT GGTGTTCAAT TAGCTGACCA TTATCAACAA 
     3181 AATACTCCAA TTGGTGATGG TCCAGTCTTG TTACCAGACA ACCATTACTT ATCCTATCAA 
     3241 TCTGCCTTAT TCAAAGATCC AAACGAAAAG AGAGACCACA TGGTCTTGTT AGAATTTTTG 
     3301 ACTGCTGCTG GTATTACCGA GGGTATGAAT GAATTGTACA AATAGTAAGA ATTCTGCAGA 
     3361 TATCCATCAC ACTGGCGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTc tatagtgtca 
     3421 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 
     3481 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 
     3541 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 
     3601 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 
     3661 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 
     3721 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     3781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     3841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt 
     3901 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     3961 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     4021 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     4081 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     4141 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 
     4201 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     4261 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     4321 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     4381 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     4441 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     4501 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 
     4561 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     4621 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     4681 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     4741 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     4801 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     4861 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     4921 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     4981 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     5041 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 
     5101 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     5161 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     5221 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     5281 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     5341 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     5401 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     5461 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     5521 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     5581 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     5641 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 
     5701 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     5761 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     5821 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     5881 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     5941 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     6001 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     6061 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     6121 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     6181 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     6241 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 
     6301 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     6361 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     6421 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     6481 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     6541 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     6601 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     6661 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     6721 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     6781 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     6841 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     6901 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     6961 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     7021 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     7081 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     7141 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     7201 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     7261 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     7321 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     7381 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     7441 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     7501 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     7561 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     7621 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     7681 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     7741 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     7801 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//