LOCUS       pcDNA3-Mito-CaNAR2.gb.xdna    7854 bp      DNA     circular   7/8/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1006..1722
                     /label=CER3
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1729..2627
                     /label=NFAT 1-297
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(5693..5713)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(2693..2710)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(6081..6709)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(5719..5741)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(6861..7520)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             4562..5353
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(7760..7788)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(5746..5775)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4343..4420
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      2733..2960
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     sig_peptide     907..996
                     /label=DAKAP-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     gene            2629..3342
                     /label=YPET
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3407..3424)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3447..3674
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG CCATCCAGTT GCGTTCGCTC TTCCCCTTGG CGTTGCCCGG AATGCTGGCC 
      961 CTCCTTGGCT GGTGGTGGTT TTTCTCTCGT AAAAAAGCGG ATCCcATGGT GAGCAAGGGC 
     1021 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC 
     1081 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG 
     1141 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG 
     1201 AGCTGGGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC 
     1261 AAGTCCGCCA TGCCCGAAGG CTACgTCcAG GAgCGCACCA TCTTCTTCAA GGACGACGGC 
     1321 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG 
     1381 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC 
     1441 GCCATCCACG GCAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC 
     1501 TTCGGCCTCA ACTGCAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG 
     1561 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG 
     1621 TCCAAGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG 
     1681 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGcgcatgcc catgaacgcc 
     1741 cccgagcggc agccccaacc cgacggcggg gacgccccag gccacgagcc tgggggcagc 
     1801 ccccaagacg agcttgactt ctccatcctc ttcgactatg agtatttgaa tccgaacgaa 
     1861 gaagagccga atgcacataa ggtcgccagc ccaccctccg gacccgcata ccccgatgat 
     1921 gtaatggact atggcctcaa gccatacagc ccccttgcta gtctctctgg cgagcccccc 
     1981 ggccgattcg gagagccgga tagggtaggg ccgcagaagt ttctgagcgc ggccaagcca 
     2041 gcaggggcct cgggcctgag ccctcggatc gagatcactc cgtcccacga actgatccag 
     2101 gcagtggggc ccctccgcat gagagacgcg ggcctcctgg tggagcagcc tcccctggcc 
     2161 ggggtggccg ccagcccgag gttcaccctg cccgtgcccg gcttcgaggg ctaccgcgag 
     2221 ccgctttgct tgagccccgc tagcagcggc tcctctgcca gcttcatttc tgacaccttc 
     2281 tccccctaca cctcgccctg cgtctcgccc aataacggcg ggcccgacga cctgtgtccg 
     2341 cagtttcaaa acatccctgc tcattattcc cccagaacct cgccaataat gtcacctcga 
     2401 accagcctcg ccgaggacag ctgcctgggc cgccactcgc ccgtgccccg tccggcctcc 
     2461 cgctcctcat cgcctggtgc caagcggagg cattcgtgcg ccgaggcctt ggttgccctg 
     2521 ccgcccggag cctcacccca gcgctcccgg agcccctcgc cgcagccctc atctcacgtg 
     2581 gcaccccagg accacggctc cccggctggg tacccccctg ttgagctcTC TAAAGGTGAA 
     2641 GAATTATTCA CTGGTGTTGT CCCAATTTTG GTTGAATTAG ATGGTGATGT TAATGGTCAC 
     2701 AAATTTTCTG TCTCCGGTGA AGGTGAAGGT GATGCTACGT ACGGTAAATT GACCTTAAAA 
     2761 TTACTCTGTA CTACTGGTAA ATTGCCAGTT CCATGGCCAA CCTTAGTCAC TACTTTAGGT 
     2821 TATGGTGTTC AATGTTTTGC TAGATACCCA GATCATATGA AACAACATGA CTTTTTCAAG 
     2881 TCTGCCATGC CAGAAGGTTA TGTTCAAGAA AGAACTATTT TTTTCAAAGA TGACGGTAAC 
     2941 TACAAGACCA GAGCTGAAGT CAAGTTTGAA GGTGATACCT TAGTTAATAG AATCGAATTA 
     3001 AAAGGTATTG ATTTTAAAGA AGATGGTAAC ATTTTAGGTC ACAAATTGGA ATACAACTAT 
     3061 AACTCTCACA ATGTTTACAT CACTGCTGAC AAACAAAAGA ATGGTATCAA AGCTAACTTC 
     3121 AAAATTAGAC ACAACATTGA AGATGGTGGT GTTCAATTAG CTGACCATTA TCAACAAAAT 
     3181 ACTCCAATTG GTGATGGTCC AGTCTTGTTA CCAGACAACC ATTACTTATC CTATCAATCT 
     3241 GCCTTATTCA AAGATCCAAA CGAAAAGAGA GACCACATGG TCTTGTTAGA ATTTTTGACT 
     3301 GCTGCTGGTA TTACCGAGGG TATGAATGAA TTGTACAAAT AGtaaGAATT CTGCAGATAT 
     3361 CCATCACACT GGCGGCCGCT CGAGCATGCA TCTAGAGGGC CCTATTctat agtgtcacct 
     3421 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 
     3481 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 
     3541 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 
     3601 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 
     3661 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 
     3721 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
     3781 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     3841 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag 
     3901 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     3961 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     4021 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     4081 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     4141 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
     4201 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     4261 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     4321 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     4381 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     4441 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     4501 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
     4561 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     4621 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     4681 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     4741 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     4801 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     4861 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     4921 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     4981 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     5041 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     5101 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     5161 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     5221 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     5281 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     5341 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 
     5401 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 
     5461 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 
     5521 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     5581 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     5641 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 
     5701 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 
     5761 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 
     5821 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
     5881 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     5941 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     6001 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     6061 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     6121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     6181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     6241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 
     6301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     6361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     6421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     6481 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 
     6541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     6601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     6661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     6721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     6781 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     6841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     6901 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     6961 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     7021 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     7081 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     7141 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     7201 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     7261 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     7321 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     7381 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     7441 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     7501 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     7561 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     7621 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     7681 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     7741 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     7801 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//