LOCUS       pcDNA3-ER-CaNAR2.gb.xdna    7855 bp      DNA     circular   7/8/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1007..1723
                     /label=CER3
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1730..2628
                     /label=NFAT 1-297
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(5694..5714)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(2694..2711)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(6082..6710)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(5720..5742)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(6862..7521)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             4563..5354
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(7761..7789)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(5747..5776)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4344..4421
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      2734..2961
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     sig_peptide     920..1000
                     /label=ER-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     gene            2630..3343
                     /label=YPET
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3408..3425)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3448..3675
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTCAGTTG 
      901 TCAGGAAGAG AAGGCTCCCA TGGACCCTGT GGTGGTGCTG GGGCTCTGTC TCTCCTGTTT 
      961 GCTTCTCCTT TCACTCTGGA AACAGAGCTA TGGGGGAGGG GATCCcATGG TGAGCAAGGG 
     1021 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG 
     1081 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT 
     1141 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT 
     1201 GAGCTGGGGC GTGCAGTGCT TCGCCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT 
     1261 CAAGTCCGCC ATGCCCGAAG GCTACgTCcA GGAgCGCACC ATCTTCTTCA AGGACGACGG 
     1321 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA 
     1381 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA 
     1441 CGCCATCCAC GGCAACGTCT ATATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA 
     1501 CTTCGGCCTC AACTGCAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA 
     1561 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA 
     1621 GTCCAAGCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT 
     1681 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGcgcatgc ccatgaacgc 
     1741 ccccgagcgg cagccccaac ccgacggcgg ggacgcccca ggccacgagc ctgggggcag 
     1801 cccccaagac gagcttgact tctccatcct cttcgactat gagtatttga atccgaacga 
     1861 agaagagccg aatgcacata aggtcgccag cccaccctcc ggacccgcat accccgatga 
     1921 tgtaatggac tatggcctca agccatacag cccccttgct agtctctctg gcgagccccc 
     1981 cggccgattc ggagagccgg atagggtagg gccgcagaag tttctgagcg cggccaagcc 
     2041 agcaggggcc tcgggcctga gccctcggat cgagatcact ccgtcccacg aactgatcca 
     2101 ggcagtgggg cccctccgca tgagagacgc gggcctcctg gtggagcagc ctcccctggc 
     2161 cggggtggcc gccagcccga ggttcaccct gcccgtgccc ggcttcgagg gctaccgcga 
     2221 gccgctttgc ttgagccccg ctagcagcgg ctcctctgcc agcttcattt ctgacacctt 
     2281 ctccccctac acctcgccct gcgtctcgcc caataacggc gggcccgacg acctgtgtcc 
     2341 gcagtttcaa aacatccctg ctcattattc ccccagaacc tcgccaataa tgtcacctcg 
     2401 aaccagcctc gccgaggaca gctgcctggg ccgccactcg cccgtgcccc gtccggcctc 
     2461 ccgctcctca tcgcctggtg ccaagcggag gcattcgtgc gccgaggcct tggttgccct 
     2521 gccgcccgga gcctcacccc agcgctcccg gagcccctcg ccgcagccct catctcacgt 
     2581 ggcaccccag gaccacggct ccccggctgg gtacccccct gttgagctcT CTAAAGGTGA 
     2641 AGAATTATTC ACTGGTGTTG TCCCAATTTT GGTTGAATTA GATGGTGATG TTAATGGTCA 
     2701 CAAATTTTCT GTCTCCGGTG AAGGTGAAGG TGATGCTACG TACGGTAAAT TGACCTTAAA 
     2761 ATTACTCTGT ACTACTGGTA AATTGCCAGT TCCATGGCCA ACCTTAGTCA CTACTTTAGG 
     2821 TTATGGTGTT CAATGTTTTG CTAGATACCC AGATCATATG AAACAACATG ACTTTTTCAA 
     2881 GTCTGCCATG CCAGAAGGTT ATGTTCAAGA AAGAACTATT TTTTTCAAAG ATGACGGTAA 
     2941 CTACAAGACC AGAGCTGAAG TCAAGTTTGA AGGTGATACC TTAGTTAATA GAATCGAATT 
     3001 AAAAGGTATT GATTTTAAAG AAGATGGTAA CATTTTAGGT CACAAATTGG AATACAACTA 
     3061 TAACTCTCAC AATGTTTACA TCACTGCTGA CAAACAAAAG AATGGTATCA AAGCTAACTT 
     3121 CAAAATTAGA CACAACATTG AAGATGGTGG TGTTCAATTA GCTGACCATT ATCAACAAAA 
     3181 TACTCCAATT GGTGATGGTC CAGTCTTGTT ACCAGACAAC CATTACTTAT CCTATCAATC 
     3241 TGCCTTATTC AAAGATCCAA ACGAAAAGAG AGACCACATG GTCTTGTTAG AATTTTTGAC 
     3301 TGCTGCTGGT ATTACCGAGG GTATGAATGA ATTGTACAAA TAGtaaGAAT TCTGCAGATA 
     3361 TCCATCACAC TGGCGGCCGC TCGAGCATGC ATCTAGAGGG CCCTATTcta tagtgtcacc 
     3421 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     3481 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     3541 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     3601 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     3661 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     3721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     3781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     3841 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta 
     3901 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     3961 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     4021 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     4081 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     4141 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     4201 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 
     4261 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     4321 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     4381 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 
     4441 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 
     4501 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 
     4561 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 
     4621 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 
     4681 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 
     4741 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 
     4801 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 
     4861 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 
     4921 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 
     4981 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 
     5041 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 
     5101 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 
     5161 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 
     5221 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 
     5281 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 
     5341 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 
     5401 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 
     5461 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 
     5521 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     5581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     5641 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 
     5701 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     5761 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     5821 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     5881 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     5941 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     6001 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     6061 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     6121 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     6181 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     6241 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 
     6301 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     6361 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     6421 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     6481 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     6541 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     6601 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     6661 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     6721 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     6781 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     6841 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     6901 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     6961 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     7021 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     7081 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     7141 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     7201 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     7261 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     7321 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     7381 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     7441 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     7501 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     7561 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     7621 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     7681 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     7741 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     7801 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//