LOCUS       pc'ER-AKAR4.xdna    7492 bp      DNA     circular   7/8/15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1007..1723
                     /label=CER
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5331..5351)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3045..3062)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5719..6347)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5357..5379)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6499..7158)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4200..4991
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7398..7426)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5384..5413)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3981..4058
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3085..3312
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     sig_peptide     920..1000
                     /label=ER-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2246..2980
                     /label=cpV-E172
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="MGGVQLADHYQQNTPIGDGPVLLXDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIE" \ 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCaa gcttCAGTTG 
      901 TCAGGAAGAG AAGGCTCCCA TGGACCCTGT GGTGGTGCTG GGGCTCTGTC TCTCCTGTTT 
      961 GCTTCTCCTT TCACTCTGGA AACAGAGCTA TGGGGGAGGg gatcccatgg tgagcaaggg 
     1021 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 
     1081 ccacaggttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 
     1141 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 
     1201 gacctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt 
     1261 caagtccgcc atgcccgaag gctacgtcca ggagcgtacc atcttcttca aggacgacgg 
     1321 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 
     1381 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 
     1441 cgccatcagt gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccca 
     1501 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 
     1561 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 
     1621 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     1681 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagcgcatgc ataagttttc 
     1741 tcaagaacag atcggcgaaa acattgtgtg cagggtcatt tgtaccacgg gtcaaattcc 
     1801 catccgagat ttgtcagctg atatttcaca agtgcttaag gaaaaacgat ccataaagaa 
     1861 agtttggaca tttggtagaa acccagcctg tgactatcat ttaggaaaca tttcaagact 
     1921 gtcaaataag catttccaaa tactactagg agaagacggt aaccttttat tgaatgacat 
     1981 ttccactaat gggacctggt taaatgggca aaaagtcgag aagaacagca atcagttact 
     2041 gtctcaaggt gatgaaataa ccgttggtgt aggcgtggaa tcagatattt tatctctggt 
     2101 cattttcata aacgacaaat ttaagcagtg cctcgagcag aacaaagttg atcgctctgc 
     2161 aggtaagcca ggcagcggcg agggcagcac caagggcctg cgtcgcgcca ccctggtgga 
     2221 cggcggcacc ggcggcagcg agctcatggg cggcgtgcag ctcgccgacc actaccagca 
     2281 gaacaccccc atcggcgacg gccccgtgct gctgccngac aaccactacc tgagctacca 
     2341 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     2401 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggaggta ccggtggatc 
     2461 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 
     2521 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 
     2581 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac 
     2641 cctcgtgacc accctgggct acggccttca gtgcttcgcc cgctaccccg accacatgaa 
     2701 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 
     2761 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 
     2821 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 
     2881 caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa 
     2941 cggcatcaag gccaacttca agatccgcca caacatcgag taagaattcT GCAGATATCC 
     3001 ATCACACTGG CGGCCGCTCG AGCATGCATC TAGAGGGCCC TATTctatag tgtcacctaa 
     3061 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 
     3121 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 
     3181 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 
     3241 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 
     3301 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 
     3361 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     3421 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     3481 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 
     3541 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     3601 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     3661 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     3721 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     3781 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg 
     3841 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     3901 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     3961 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     4021 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
     4081 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 
     4141 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 
     4201 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 
     4261 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 
     4321 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 
     4381 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 
     4441 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 
     4501 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 
     4561 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 
     4621 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 
     4681 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 
     4741 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 
     4801 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 
     4861 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 
     4921 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 
     4981 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 
     5041 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 
     5101 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 
     5161 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     5221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     5281 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 
     5341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     5401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     5461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     5521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     5581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     5641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     5701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     5761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     5821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     5881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 
     5941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     6001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     6061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     6121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
     6181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     6241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     6301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     6361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     6421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     6481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     6541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     6601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     6661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     6721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     6781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     6841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     6901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     6961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     7021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     7081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     7141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     7201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     7261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     7321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     7381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     7441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//