LOCUS       pSP-G1       7351 bp dsDNA    Circular
COMMENT     This file is created by Vector NTI 4.0 from InforMax, Inc., USA:
COMMENT     (800)357-3114 or http://www.informaxinc.com/
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FEATURES               Location/Qualifiers
     CDS             417..1217
                     /label=URA3
     rep_origin      5943..7098
                     /label=2µ\ori
     CDS             complement(4952..5809)
                     /label=ampR\
     rep_origin      1483..1789
                     /label=f1\ori
     terminator      1885..2049
                     /label=T\ADH1\
     rep_origin      4134..4801
                     /label=pUC\ori
     terminator      3758..3947
                     /label=T\CYC1\
     promoter        2672..3655
                     /label=P\PGK1
     promoter        complement(2243..2662)
                     /label=P\TEF1
     misc_feature    complement(2198..2221)
                     /label=FLAG\epitope
     misc_feature    3699..3728
                     /label=myc\epitope
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     1801 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 
     1861 ttcgctatta cgccagctga attggagcga cctcatgcta tacctgagaa agcaacctga 
     1921 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt 
     1981 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata 
     2041 agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca 
     2101 tcttttcgta aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca 
     2161 aacctctggc gaagaattgt taattaagag ctcagatctt atcgtcgtca tccttgtaat 
     2221 ccatcgatac tagtgcggcc gcttgtaatt aaaacttaga ttagattgct atgctttctt 
     2281 tctaatgagc aagaagtaaa aaaagttgta atagaacaag aaaaatgaaa ctgaaacttg 
     2341 agaaattgaa gaccgtttat taacttaaat atcaatggga ggtcatcgaa agagaaaaaa 
     2401 atcaaaaaaa aaaattttca agaaaaagaa acgtgataaa aatttttatt gcctttttcg 
     2461 acgaagaaaa agaaacgagg cggtctcttt tttcttttcc aaacctttag tacgggtaat 
     2521 taacgacacc ctagaggaag aaagagggga aatttagtat gctgtgcttg ggtgttttga 
     2581 agtggtacgg cgatgcgcgg agtccgagaa aatctggaag agtaaaaaag gagtagaaac 
     2641 attttgaagc tatggtgtgt gcggccggcc tggaagtacc ttcaaagaat ggggtcttat 
     2701 cttgttttgc aagtaccact gagcaggata ataatagaaa tgataatata ctatagtaga 
     2761 gataacgtcg atgacttccc atactgtaat tgcttttagt tgtgtatttt tagtgtgcaa 
     2821 gtttctgtaa atcgattaat ttttttttct ttcctctttt tattaacctt aatttttatt 
     2881 ttagattcct gacttcaact caagacgcac agatattata acatctgcat aataggcatt 
     2941 tgcaagaatt actcgtgagt aaggaaagag tgaggaacta tcgcatacct gcatttaaag 
     3001 atgccgattt gggcgcgaat cctttatttt ggcttcaccc tcatactatt atcagggcca 
     3061 gaaaaaggaa gtgtttccct ccttcttgaa ttgatgttac cctcataaag cacgtggcct 
     3121 cttatcgaga aagaaattac cgtcgctcgt gatttgtttg caaaaagaac aaaactgaaa 
     3181 aaacccagac acgctcgact tcctgtcttc ctattgattg cagcttccaa tttcgtcaca 
     3241 caacaaggtc ctagcgacgg ctcacaggtt ttgtaacaag caatcgaagg ttctggaatg 
     3301 gcgggaaagg gtttagtacc acatgctatg atgcccactg tgatctccag agcaaagttc 
     3361 gttcgatcgt actgttactc tctctctttc aaacagaatt gtccgaatcg tgtgacaaca 
     3421 acagcctgtt ctcacacact cttttcttct aaccaagggg gtggtttagt ttagtagaac 
     3481 ctcgtgaaac ttacatttac atatatataa acttgcataa attggtcaat gcaagaaata 
     3541 catatttggt cttttctaat tcgtagtttt tcaagttctt agatgctttc tttttctctt 
     3601 ttttacagat catcaaggaa gtaattatct actttttaca acaaatataa aacaaggatc 
     3661 cgtaatacga ctcactatag ggcccgggcg tcgacatgga acagaagttg atttccgaag 
     3721 aagacctcga gtaagcttgg taccgcggct agctaagatc cgctctaacc gaaaaggaag 
     3781 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa 
     3841 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta 
     3901 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaagatc cagctgcatt 
     3961 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     4021 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     4081 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     4141 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     4201 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     4261 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     4321 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     4381 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     4441 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     4501 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     4561 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     4621 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     4681 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     4741 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     4801 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     4861 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     4921 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     4981 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     5041 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     5101 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     5161 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     5221 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     5281 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     5341 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     5401 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     5461 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     5521 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     5581 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     5641 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     5701 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     5761 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     5821 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     5881 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     5941 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt 
     6001 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt 
     6061 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa 
     6121 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc 
     6181 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc 
     6241 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg 
     6301 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg 
     6361 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta 
     6421 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat 
     6481 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag 
     6541 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca 
     6601 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa 
     6661 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg 
     6721 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga 
     6781 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc 
     6841 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc 
     6901 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt 
     6961 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac 
     7021 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg 
     7081 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct 
     7141 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta 
     7201 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct 
     7261 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat 
     7321 aaaaataggc gtatcacgag gccctttcgt c 
//
