LOCUS       pKK01                  11960 bp    DNA     circular     23-NOV-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|643472871|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     CDS             complement(2339..3530)
                     /vntifkey="4"
                     /label=pha\A
     promoter        complement(3545..4528)
                     /vntifkey="30"
                     /label=P\PGK1
     promoter        4538..4957
                     /vntifkey="30"
                     /label=P\TEF1
     misc_marker     complement(7004..7861)
                     /vntifkey="22"
                     /label=AmpR
     rep_origin      7995..9150
                     /vntifkey="33"
                     /label=2\micron\origin
     rep_origin      6186..6853
                     /vntifkey="33"
                     /label=pUC\origin
     terminator      complement(5783..5972)
                     /vntifkey="43"
                     /label=T\CYC1
     terminator      2030..2194
                     /vntifkey="43"
                     /label=T\ADH1
     rep_origin      1602..1908
                     /vntifkey="33"
                     /label=f1\origin
     misc_marker     536..1336
                     /vntifkey="22"
                     /label=URA3
     CDS             4964..5714
                     /vntifkey="4"
                     /label=pha\B
     CDS             complement(9609..11388)
                     /vntifkey="4"
                     /label=pha\C
     terminator      9400..9589
                     /vntifkey="43"
                     /label=T\CYC\1
     promoter        complement(11465..11884)
                     /vntifkey="30"
                     /label=P\TEF1
BASE COUNT     3409 a      2605 c      2597 g      3349 t 
ORIGIN
        1 aattggatta gtctcatcct tcaatgctat catttccttt gatattggat catactaaga 
       61 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 
      121 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 
      181 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 
      241 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 
      301 ccataccaca gcttttcaat tcaattcatc attttttttt tattcttttt tttgatttcg 
      361 gtttctttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga 
      421 gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc 
      481 agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc 
      541 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt 
      601 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa 
      661 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt 
      721 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc 
      781 caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa 
      841 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca 
      901 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa 
      961 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg 
     1021 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt 
     1081 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc 
     1141 cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga 
     1201 tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag 
     1261 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag 
     1321 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca 
     1381 aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa ataccgcaca 
     1441 gatgcgtaag gagaaaatac cgcatcagga aattgtaaac gttaatattt tgttaaaatt 
     1501 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 
     1561 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 
     1621 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 
     1681 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 
     1741 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 
     1801 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 
     1861 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 
     1921 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 
     1981 tcgctattac gccagctgga taaaggcgcg ccaaacgacc taggaattgg agcgacctca 
     2041 tgctatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa taagaatttt 
     2101 cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt tataacttat 
     2161 ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat 
     2221 ctaccaacga tttgaccctt ttccatcttt tcgtaaattt ctggcaaggt agacaagccg 
     2281 acaaccttga ttggagactt gaccaaacct ctggcgaaga attgttaatt aagagctctc 
     2341 actttctttc caccgccaat gcaacgccca taccaccccc aatacataaa gaagctaaac 
     2401 cctttttggc atctctacgt ttcatttcat gcaacaatgt taccaaaatc ctacatcccg 
     2461 aagcacctat aggatggcct atagcaatcg ctccaccgtt aacgtttacc ttagaggtat 
     2521 cccaacccat ttgctggtgc accgctagag cctgtgctgc aaaagcttca ttgatttcca 
     2581 tcaagtccaa atcctgggga gtccactccg ctctggacaa agcccttttc gaagctggca 
     2641 caggacccat acccataact tttgggtcaa caccggcgtt ggcatatgat ttaatagttg 
     2701 caagaggggt taaacccaat tccttagcct tggctgcact catgacaacg acagcagctg 
     2761 ctccatcatt tagacctgaa gcgtttgctg ctgtaacggt gcccgcctta tcaaatgcag 
     2821 gctttaatcc gctcattgaa tctaaagtag caccttgtct aacaaactca tctgttttga 
     2881 aagccactgg atcacctttc ctctgtggta ttaacactgg aacaatttct tcgtcaaatt 
     2941 tcccagcctt ttgagcagct tctgccttat tttgggaacc tacggcgaat tcatcttgag 
     3001 cttctctcgt aattccatat tctttagcca cgttctcggc cgtaataccc atatggtact 
     3061 gattgtatac atcccaaaga ccatctacta tcatagtatc cacaagttta gcgtcaccca 
     3121 ttctaaaccc atccctagaa cccggcagga catgaggagc agcagacatg ttttcttggc 
     3181 ctccggcaac cacaatctcg gcatcaccgg ccatgatagc atttgcggct aacattacgg 
     3241 ccttcaaacc tgaaccacac accttattaa tcgtcattgc aggcaccatc gcaggtagtc 
     3301 ctgccttaat tgctgcttgc ctcgcagggt tctggcctga acccgctgtt aggacttgac 
     3361 ccataattac ttcgctcact tgttctggtt ttactcctgc cctttctaaa gctgccttta 
     3421 taacgacagc ccccaattcg ggagctggaa ttttggctaa agaaccacca aacttaccaa 
     3481 ctgctgtcct agctgcagat acaataacca catcggtcat tttgtttgaa actagtgcgg 
     3541 ccgcttgttt tatatttgtt gtaaaaagta gataattact tccttgatga tctgtaaaaa 
     3601 agagaaaaag aaagcatcta agaacttgaa aaactacgaa ttagaaaaga ccaaatatgt 
     3661 atttcttgca ttgaccaatt tatgcaagtt tatatatatg taaatgtaag tttcacgagg 
     3721 ttctactaaa ctaaaccacc cccttggtta gaagaaaaga gtgtgtgaga acaggctgtt 
     3781 gttgtcacac gattcggaca attctgtttg aaagagagag agtaacagta cgatcgaacg 
     3841 aactttgctc tggagatcac agtgggcatc atagcatgtg gtactaaacc ctttcccgcc 
     3901 attccagaac cttcgattgc ttgttacaaa acctgtgagc cgtcgctagg accttgttgt 
     3961 gtgacgaaat tggaagctgc aatcaatagg aagacaggaa gtcgagcgtg tctgggtttt 
     4021 ttcagttttg ttctttttgc aaacaaatca cgagcgacgg taatttcttt ctcgataaga 
     4081 ggccacgtgc tttatgaggg taacatcaat tcaagaagga gggaaacact tcctttttct 
     4141 ggccctgata atagtatgag ggtgaagcca aaataaagga ttcgcgccca aatcggcatc 
     4201 tttaaatgca ggtatgcgat agttcctcac tctttcctta ctcacgagta attcttgcaa 
     4261 atgcctatta tgcagatgtt ataatatctg tgcgtcttga gttgaagtca ggaatctaaa 
     4321 ataaaaatta aggttaataa aaagaggaaa gaaaaaaaaa ttaatcgatt tacagaaact 
     4381 tgcacactaa aaatacacaa ctaaaagcaa ttacagtatg ggaagtcatc gacgttatct 
     4441 ctactatagt atattatcat ttctattatt atcctgctca gtggtacttg caaaacaaga 
     4501 taagacccca ttctttgaag gtacttccag gccggccgca cacaccatag cttcaaaatg 
     4561 tttctactcc ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt 
     4621 caaaacaccc aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa 
     4681 ttacccgtac taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc 
     4741 gaaaaaggca ataaaaattt ttatcacgtt tctttttctt gaaaattttt ttttttgatt 
     4801 tttttctctt tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc 
     4861 aagtttcagt ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa 
     4921 agaaagcata gcaatctaat ctaagtttta attacaagga tccttcaaac aaaatgacac 
     4981 aaagaattgc atatgtaact ggcggaatgg gtggaatcgg tacagcaatt tgccagcgtt 
     5041 tagcaaagga tggtttcaga gtggttgctg gttgcggtcc aaatagtcct cgtagagaga 
     5101 agtggctgga gcaacaaaaa gccttagggt ttgactttat tgccagcgaa ggtaatgtag 
     5161 cagattggga ctctaccaaa acggcttttg acaaagttaa gtcagaagtc ggggaagtag 
     5221 atgttttgat taacaatgcg ggtataactc gtgatgtcgt gtttaggaag atgactagag 
     5281 cagactggga tgctgttata gatacaaatc ttacatcgct atttaatgtt acaaagcaag 
     5341 tgattgatgg catggccgat aggggctggg gaagaatcgt aaacatcagt tcggtcaatg 
     5401 gtcagaaagg tcagttcgga cagaccaatt attctacggc taaggctggg ttgcatggct 
     5461 tcactatggc attggctcaa gaagttgcaa cgaaaggtgt aactgtaaat acagtctccc 
     5521 caggatatat tgcaaccgat atggtgaagg caattaggca agacgttcta gacaagattg 
     5581 tcgccactat accagttaag aggttaggtt tgcctgaaga aatagcttca atttgcgctt 
     5641 ggctttccag tgaggaatcg ggttttagta caggtgcaga tttctcctta aacggtggtc 
     5701 tacacatggg ttaggtcgac atggaacaga agttgatttc cgaagaagac ctcgagtaag 
     5761 cttggtaccg cggctagcta agatccgctc taaccgaaaa ggaaggagtt agacaacctg 
     5821 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat 
     5881 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa 
     5941 ccttgcttga gaaggttttg ggacgctcga agatcctccg gatcgtttcg ccggcgttta 
     6001 tccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     6061 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     6121 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     6181 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     6241 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     6301 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     6361 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     6421 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     6481 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     6541 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     6601 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     6661 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     6721 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     6781 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     6841 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     6901 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     6961 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     7021 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     7081 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     7141 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     7201 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     7261 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     7321 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     7381 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     7441 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     7501 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     7561 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     7621 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     7681 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     7741 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     7801 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     7861 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     7921 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     7981 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga 
     8041 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 
     8101 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg 
     8161 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca 
     8221 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg 
     8281 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg 
     8341 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga 
     8401 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt 
     8461 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata 
     8521 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc 
     8581 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag 
     8641 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt 
     8701 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat 
     8761 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat 
     8821 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag 
     8881 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt 
     8941 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac 
     9001 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 
     9061 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 
     9121 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 
     9181 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 
     9241 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgt cagtgagcga 
     9301 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 
     9361 atgcagctgg ataaacgccg gcgaaacgat ccggaggatc ttcgagcgtc ccaaaacctt 
     9421 ctcaagcaag gttttcagta taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag 
     9481 aaaaatttga aatataaata acgttcttaa tactaacata actataaaaa aataaatagg 
     9541 gacctagact tcaggttgtc taactccttc cttttcggtt agagcggatc ttagctagcc 
     9601 gcggtaccct atgcttttgc cttaacatac ctaccgggtg ccggctctat tgccctatat 
     9661 cttgcattcc cgtaattggc gggtgcagca cgttttgctc cggcttgtcc agctagccag 
     9721 gctgtccagt caggccacca agagccatga tgttctatcg caccggccaa ccattgttgt 
     9781 ggtgactctg gtaaagcatc atttgtccaa tgagaacgtt tgtttttagc aggaggattg 
     9841 attactcctg ctatatgtcc agaggctccc aaaacgaacc ttaacttgtt ggccaaaagt 
     9901 gcggtagaag cgtatgctgc agtccaagga acaatgtgat cttctctgct tccgtaaatg 
     9961 taagtgggaa cgtctatact tgctaaatcg actggaactc cacacactgt caatttaccg 
    10021 ggaaccttta actcattttg caagtatgta tgtcttagat accaacagta ccagggacct 
    10081 ggcaagttgg tagcatcacc gttccagaac aacaagtcga aaggaacagg agtattgcct 
    10141 ttcaagtagt tatcaacgac gtagttccaa actaggtcgt taggccttaa aaagctgaat 
    10201 gtgtttgcta attctaaacc acgaagtagg gcacaaggcg ctcctgcgcc acctcccaaa 
    10261 gtcgcctcac gtagttgaac atgaccctcg tcaacaaaca catcaagtat tccagtatca 
    10321 gcaaaatcta gtagtgtggt taatagtgtt acgcttgctg caggatgttc tccccttgca 
    10381 gcaagaactg ccaacgcggt tgaaacgata gtacccccga cgcaaaaccc taatacattg 
    10441 attttatctt gaccagagat atccctagca acttcgatag ctcttattgc ggcatgttct 
    10501 atataatcat cccatgttga acccgccatc gaagcatcgg gatttctcca actaaccaaa 
    10561 aatactgtgt gaccttgttc tactacatgt ctaacaagtg aggattcagg ctgcaaatca 
    10621 aggatgtaat acttgttgat acaaggagga accatcaaca gaggcctagc gtgtacttta 
    10681 tctgtcaatg gtttgtattg caaaagttga aaatattcat tttcaaaaac gacagctccc 
    10741 tcggtaaccg caacgtttct acccacttca aaagctgatt catcagtttg ggatattttt 
    10801 ccccttgtta aatcctccat catatttctc acacctgcac gtaaactctc gccacctgat 
    10861 tcaattaaca gtctttgtgc ttcaggattt gtagccaaga aatttgcagg actcattgca 
    10921 tctacccatt gagatattgc gaacctgatt ctctgacgcg ttttagcatc cgcttcgact 
    10981 gcatcggcta gctctgtaag ggctcttgca ttcagtaaat agaaggccgc tgcgaatctg 
    11041 tatggcaaat tggtcctcca tgcgtctcca gcaaatctcc tatcatgcag tggacctgta 
    11101 gcttcagcct tcccttcagc catggcctgc caaagtgcgg aaaaatcttt catatatctc 
    11161 tgttggatat cgccaagctg agctggagct attttaacac ctgctaaggc atctaaacct 
    11221 ggaataccgg aggcagctgc atggccatta ccctctgtac cctgccattg cctggaccat 
    11281 tccagccagg tagctggatc aaaagggcca ggtgtaacct taaagggttg tgattttcct 
    11341 tcttgagtag atgcagcagc acctttacca gttgccattt tgtttgaact cgaggtcttc 
    11401 ttcggaaatc aacttctgtt ccatgtcgac gcccgggccc tatagtgagt cgtattacgg 
    11461 atccttgtaa ttaaaactta gattagattg ctatgctttc tttctaatga gcaagaagta 
    11521 aaaaaagttg taatagaaca agaaaaatga aactgaaact tgagaaattg aagaccgttt 
    11581 attaacttaa atatcaatgg gaggtcatcg aaagagaaaa aaatcaaaaa aaaaaatttt 
    11641 caagaaaaag aaacgtgata aaaattttta ttgccttttt cgacgaagaa aaagaaacga 
    11701 ggcggtctct tttttctttt ccaaaccttt agtacgggta attaacgaca ccctagagga 
    11761 agaaagaggg gaaatttagt atgctgtgct tgggtgtttt gaagtggtac ggcgatgcgc 
    11821 ggagtccgag aaaatctgga agagtaaaaa aggagtagaa acattttgaa gctatggtgt 
    11881 gtgcggccgg cctggaagta ccttcaaaga atggggtctt atcttgtttt gcaagtacca 
    11941 ctgagcagga taataatagc 
//
